Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 123 for “"Open reading frames"”.
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Characterisation of open reading frames m29 and m29.1 in murine cytomegalovirus
… (HCMV). Of the predicted 170 MCMV open reading frames (ORFs) only 78 have significant amino acid identity with genes in HCMV. To better understand the biological mechanisms underlying the differences between the viruses, for example their species specificity and immune evasion …
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The toxigenic element of Clostridium difficile strain 10463 and its transcriptional analysis in strains which differ in toxigenicity
… is 19.6 kb in length and is comprised of five open reading frames, including the toxin A and B genes. One of these open reading frames is previously unidentified and is located upstream of toxin B. Products of Polymerase Chain Reaction (PCR) amplification of three regions in the toxigenic …
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Analysis of a Cotton Gene Cluster for the Antifungal Protein Osmotin
… The two cotton osmotin genes were found to have open reading frames of 729 basepairs without any introns, and would encode presumptive osmotin preproteins of 242 amino acids. The open reading frames of the genes are identical in sequence to two corresponding cDNA clones (GenBank Accessions …
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DNA comparisons of the two orthopoxviruses monkeypox and variola
… Analysis of the insert revealed two overlapping open reading frames present on opposite DNA strands. The DNA and putative polypeptide sequences were compared with known sequences, but no significant homology was detected. The presence or absence of this sequence in other orthopoxviruses is being …
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Genetische Organisation und Transkription eines Virulenz-assoziierten, instabilen Chromosomenabschnitts von Listeria ivanovii
… dem bereits charakterisierten Gen smcL 13 neue Open Reading Frames (ORFs) bzw. Gene (ydeI, rnaH, norA) von L. ivanovii identifiziert werden, die größtenteils in der Deletionsmutante L. ivanovii GD-3 deletiert waren. Für die meisten Open Reading Frames konnten Homologien zu ORFs in den …
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The Role of an Ultraconserved Long Non-coding Rna in B-Cell Lymphomagenesis
… (ncPEPs). These peptides, originating from small open reading frames (smORFs), contribute to diverse cellular functions and are implicated in disease pathogenesis.</p>
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Experimental investigations of mastrevirus molecular biology and evolution
… of interactions between the two virion-sense open reading frames (ORFs) of MSV, their products, and the rest of the genome. Six chimaeric MSV constructs were made by reciprocally exchanging the ORFs encoding movement protein (MP) and coat protein (CP) individually, and in pairs, between …
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The Regulation of NAP4 in Saccharomyces cerevisiae
… regulation via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 …
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Molecular cloning, structural characterisation and functional study of bioactive peptides from amphibian skin secretion
… full length nucleotide sequence that encoded the open reading frames of peptide biosynthetic precursors. Three putative peptides were identified by cloning (Kunitzin-AH, QUB-1617, QUB-1629), and each was confirmed by MALDI-TOF mass spectrometry and RPHPLC, for mass confirmation and purification. …
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Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12
… <i>GlpR</i> and <i>glpD</i> are separated by two open reading frames, designated <i>glpE</i> and <i>glpG</i>, encoding proteins of unknown function. The <i>glpD</i>-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide …
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Development of a ribosome profiling protocol to study translation in the yeast Kluyveromyces marxianus
… of mRNA translation including recoding (e.g. +1 frameshifting), ribosome stalling and differential gene expression between multiple conditions. In addition, as RPFs can be mapped back to the genome, novel translated ORFs may be discovered including novel protein coding genes and regulatory …
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A study of mammalian microRNA-mediated repression of gene expression by ribosome profiling
… we observed widespread translation of upstream open reading frames initiated from canonical and non-canonical start codons. These upstream open reading frames are also translationally repressed by miR-155.
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The capsular polysaccharide of Actinobacillus pleuropneumoniae serotype 5A: role in serum resistance and characterization of the genetic basis for expression
… A. pleuropneumoniae serotypes and contained four open reading frames, cpxDCBA, that were highly homologous at both the nucleotide and amino acid levels to genes involved in the export of the CP of H. influenzae type b (bexDCBA), Neisseria meningitidis group B (ctrABCD), and to a lesser extent …
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Phenotypic and genotypic relationship between tabtoxin production and pathogenicity in Pseudomonas syringae pv. tabaci strain PTBR2.024
… phenotypes. This 3-kb fragment contains two open reading frames (ORF), ORF1 and ORF2, and two truncated ORFs, ORF3 and ORF4. The Tn5 insert in PTBR7.000 was mapped to ORF2, and complementation studies showed that an intact ORF2 was sufficient to restore tabtoxin production and pathogenicity. …
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Temporal Analysis of Bacteriophage Felix O1 Gene Expression
… reaction was performed. All of the 115 putative open reading frames (ORFs) studied were found to be functional. 53.0%, 9.6%, and 18.3% of the ORFs investigated were found to initiate expression early, middle or late in the lytic cycle, respectively. Expression of the remaining 19.1% ORFs was …
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Mixed Strain Identification of Porcine Reproductive and Respiratory Syndrome Virus in Multiplexed Samples using Nanopore Sequencing
… developed a novel primer set designed to span Open Reading Frames 3 through 7 of the PRRSV genome, which has allowed for multiplexing of samples, thus reducing individual cost of testing, while yielding significantly more information than previously available. This novel primer pair and …
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Characterization of the Factors Associated with SCCmec Mobility in Staphylococcus Aureus
… strain and appaers to be prone to recomination. Open reading frames found in this region were homologous to enterotoxins, restriction-modification enzymes, and transposases. Several open reading frames that have not been previously reported in staphylococci were also present in this region. 28 …
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Gene expression profiling in Saccharomyces cerevisiae grown at different specific gravity environments
… microarray analysis of ~ 6200 yeast open reading frames. Genomics analysis showed that 400 genes in S. cerevisiae was differentially expressed by more than 1.5-fold compared with controls at late-logarithmic phase of fermentation, as the yeast adapted to changing nutritional, …
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Identification of genes from the poxvirus of a parasitoid wasp
… doubling the number of know DlEPV genes. DlEPV open reading frames (ORFs) were found to have significant homology to both essential and nonessential poxvirus genes. We also report the presence of a putative NAD+-dependent DNA ligase, only the second example of this gene from a virus. DlEPV …
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