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Showing 1 to 20 of 288 for “"Oligonucleotides"”.
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Studies on Anthracene Tagged Oligonucleotides
… characterization and study of a range of oligonucleotides that were modified by the inclusion of a non-nucleosidic anthracene unit. This involved the synthesis of different stereoisomers of a non-nucleosidic backbone attached to DNA via a carbon chain linker. These monomers were then …
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Valence and structure relationships in oligonucleotides
<p>There are many models that can be used to estimate the degree of shielding and predict the valence of a nucleic acid. Here, Counter-ion Condensation theory and Grand Canonical Monte Carlo simulations are compared with an experimental approach. Membrane-Confined Electrophoresis was used to …
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Selective targeting of MYC by antisense oligonucleotides
… goals of this research were to develop antisense oligonucleotides specifically targeted against the MYC mRNA to achieve potent inhibition of MYC translation, and to characterize the activity of these molecules as specific modulators of MYC expression and as prototypical MYC-directed therapeutics. …
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Modified oligonucleotides for the functionalisation of nanoscale materials
… a suitable linker group with which to tether oligonucleotides to nanoparticle substrates. As such, routes to the modification of oligonucleotides by thioctic acid are reported herein. Although the phosphoramidite method was found to be unsuccessful, the H-phosphonate method has been shown to …
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Intermolecular Joining of Dna Oligonucleotides With Rna Ligase
Made available in DSpace on 2014-12-14T04:27:48Z (GMT). No. of bitstreams: 1 8009101.pdf: 6452301 bytes, checksum: 2b7980a57152a1c82231d5c8a4574206 (MD5) Previous issue date: 1979
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Covalent end-immobilization of oligonucleotides onto solid surfaces
… for DNA assays. For this purpose I synthesized oligonucleotides (oligos) stepwise onto derivatized SiO2 surfaces. Then double-stranded (ds)DNA molecules with "dangling end" oligo overhangs were immobilized onto the oligo surface by hybridization. Photolysis of psoralen crosslinkers covalently …
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Conjugation of oligonucleotides for drug delivery via a linker derived from Meldrum’s acid and A capture-release method to synthesize long oligonucleotides
… to simplify the isolation of full-length long oligonucleotides. For this project we aimed for the synthesis of a propargylated glycerol phosphoramidite as a 5’-end label. Incorporation of this alkyne into oligonucleotides by solid-phase synthesis will enable the capture of the full-length …
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Synthesis and bio-pharmacological activity of antisense phosphatidyl-oligonucleotides
Lipid conjugated oligonucleotides are of great interest in the field of antisense oligonucleotides used for functional genomics, gene target validation and therapeutic pourpose. Although various lipid conjugates of oligonucleotides have already been prepared, it was not possible until now to …
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Crystallographic studies of interactions between ligands and DNA oligonucleotides
… with d(CGCAAATTTGCG)2 and d(CGCGAATTCGCG)2 oligonucleotides, and is divided into two sections. Section A includes seven structures with Halogenated Hoechst 33258 analogues that are potential agents in radiotherapy, phototherapy, radioimmunotherapy or photoimmunotherapy, and the structure of …
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Strategic modification of oligonucleotides with carboxylic acids containing small molecules
Small interfering RNA (siRNA) are double-stranded RNA molecules used to manipulate gene regulation, by silencing gene expression. SiRNA molecules have been integrated into the therapeutic field, with several FDA-approved medications targeting liver-specific diseases. While effective, these …
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Improving Cardiac Delivery of Antisense Oligonucleotides with Peptidomimetic Targeting Agents
… Here, we investigate the delivery of antisense oligonucleotides (ASOs) to inhibit the expression of an overexpressed miRNA in CVD, miRNA-21. One of the challenges in delivering ASOs and other gene therapies is achieving delivery to the desired tissue before the therapeutic is trafficked to the …
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Studies on the detection of nucleotides and oligonucleotides by mass spectrometry
… novel methods for the detection of nucleotides, oligonucleotides, and modified nucleotides such as DNA adducts by mass spectrometry. DNA adducts are important because they are formed during chemical carcinogenesis as well as during anti-cancer chemotherapy. However, DNA adducts are not routinely …
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Gold Nanoparticles as a Delivery System of Oligonucleotides into the Brain
… gold glyconanoparticles as a delivery system for oligonucleotides into the brain. Three ligand formulations of gold glyconanoparticles were investigated, covalently coated with glucose, OEG-amine/galactose or OEG-amine/galactose/insulin. The two formulations with OEG-amine showed higher uptake …
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Peptide-mediated delivery of antisense oligonucleotides and chemotherapeutics across biological barriers
… for the peptide-mediated delivery of antisense oligonucleotides (ASOs) and chemotherapeutics. First, we address the issue that the optimal peptide sequence for the delivery of a macromolecular cargo is often context-dependent and specific to that cargo. With one class of ASO, we develop a …
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SPINAL MUSCULAR ATROPHY ORGANOIDS REVEAL DEVELOPMENTAL DEFECTS RESCUED BY ANTISENSE OLIGONUCLEOTIDES TREATMENT
Spinal muscular atrophy (SMA) is a neuromuscular disease caused by mutations in the SMN1 gene. Recent therapies have significantly modified SMA natural course, but treatment efficacy remains variable and the reasons beyond this variability are still largely unexplored. Identifying pre-symptomatic …
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RACE-Seq identifies the Argonaute-2 cleavage products of RNA interference-based oligonucleotides
… in the field. Synthetic RNAi therapeutic oligonucleotides are either first cleaved by Dicer or incorporate directly into the Argonaute-2 RNA-induced silencing complex (AGO2-RISC) and directs the protein complex to homologous RNA. Cleavage of target RNA occurs opposite bases 10-11 when …
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Development of particle-based systems for the delivery of oligonucleotides into primary dendritic cells
… a particulate-based vector for delivery of oligonucleotides (ODN) into dendritic cells using a cationic peptide (O10H6) to provide enhanced delivery. Anchoring of O10H6-ODN condensates was hypothesized to provide increased intracellular ODN accumulation required in decoy therapy. The …
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A technique to randomise consecutive codons in a sequence of DNA using MAX oligonucleotides
… in a DNA strand. The method makes use of “MAX” oligonucleotides coding for each of the 20 amino acids that are ligated to a conserved sequence of DNA using T4 DNA ligase. The “MAX” oligonucleotides were synthesised in such a way, with an MlyI restriction site, that restriction of the …
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Comparison of Catalogues of Purine-Rich Oligonucleotides From Escherichiacoli and Bacillus Subtilis Ribosomal Ribonucleic Acids
Made available in DSpace on 2014-12-09T22:04:23Z (GMT). No. of bitstreams: 1 7013416.pdf: 1718497 bytes, checksum: cfe43de620c8c53f71e165f15ea412e8 (MD5) Previous issue date: 1969
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