Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Oligonucleotide Design"”.
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Oligonucleotide Design for Whole Genome Tiling Arrays
Oligonucleotides are short, single-stranded fragments of DNA or RNA, designed to readily bind with a unique part in the target sequence. They have many important applications including PCR (polymerase chain reaction) amplification, microarrays, or FISH (fluorescence in situ hybridization) probes. …
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Oligonucleotide design and codon optimization for PCR-based gene synthesis
… This thesis introduces a software tool designed to make it simpler and more reliable. DNA is synthesized from overlapping oligonucleotides by ligation or PCR; this thesis focuses on PCR-based methods. Many sets of oligonucleotides can be used to synthesize a given sequence; choosing the …
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Typing and genome comparison of Neisseria meningitidis by DNA-microarrays
… microarrays based on polymorphism-directed oligonucleotide design for typing of N. meningitidis adopting the multilocus sequence typing (MLST) concept. The number of oligonucleotides needed to cover all known polymorphisms was much lower compared to the number needed if a tiling strategy …
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A systematic and extensible approach to DNA primer design for whole gene synthesis
… in the purification of solid-phase manufactured oligonucleotides make it possible to manufacture whole genes by polymerase chain reaction methods. Yet, despite the improvement in laboratory methods, whole gene synthesis is not rapidly progressing because most gene design software takes an …
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Creating Scientific Software, with Application to Phylogenetics and Oligonucleotide Probe Design
The demands placed on scientific software are different from those placed on general purpose software, and as a result, creating software for science and for scientists requires a specialized approach. Much of software engineering practices have developed in situations in which a tool is desired to …
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Towards automatic gene synthesis with bioinformatics software, novel one-step real-time PCR assembly, and lab-chip gene synthesis
… software TmPrime is developed to optimize oligonucleotide design. It is able to design oligonucleotides with homologous melting temperature for both LCR and gapless PCR assembly of very long gene sequences. The potential mis-hybridization, hetero-dimer, homo-dimer and hairpin formations …
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Characterization of Oligonucleotide Microarray Hybridization: Microarray Fabrication by Light-Directed in situ Synthesis – Development of an Automated DNA Microarray Synthesizer, Characterization of Single Base Mismatch Discrimination and the Position-Dependent Influence of Point Defects on Oligonucleotide Duplex Binding Affinities
… target sequences and complementary end-tethered oligonucleotide probes on the surface of DNA microarrays. Hybridization experiments were performed on oligonucleotide microarrays (DNA Chips) which were fabricated with an automated synthesis apparatus (developed in the framework of the present …