Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 26 for “"Oligodeoxynucleotides"”.
-
Enhancing spinal cord repair with connexin43 antisense oligodeoxynucleotides
… The segments were treated with Cx43 antisense oligodeoxynucleotides (Cx43 AsODN) resulting in viable, organotypic, airliquid interface cultures that were then used for repair studies. Fresh peripheral nerves were grafted into the spinal cord segments with Cx43AsODN applied concomitantly, …
-
CNS delivery of antisense oligodeoxynucleotides using biodegradable microspheres
Antisense oligodeoxynucleotides can selectively inhibit gene expression provided they are delivered to their target site successfully for a sufficient duration. Biodegradable microspheres have previously been developed for the potential systemic delivery of antisense oligodeoxynucleotides and offer …
-
The sustained delivery of antisense oligodeoxynucleotides using biodegradable polymer microspheres
… a wide range of diseases. The efficacy of naked oligodeoxynucleotides (ODNs) is relatively short lived due to rapid degradation in vivo. The entrapment of ODNs within biodegradable sustained-release delivery systems may improve ODN stability and reduce dose required for efficacy. Biodegradable …
-
LOCOREGIONAL DELIVERY OF UNMETHYLATED CPG-OLIGODEOXYNUCLEOTIDES TO CANCER THERAPY: PRECLINICAL STUDIES
Tumor cell growth, even in advanced stages of ovarian cancer, is nearly always restricted to the peritoneal cavity, therefore repeated intraperitoneal injections of CpG-ODN recruiting and activating innate effector cells throughout the abdominal cavity to the tumor site might control tumor cell …
-
Immunostimulatory properties and mechanisms of action of encapsulated methylated cpg oligodeoxynucleotides
Immunostimulatory oligodeoxynucleotides (ODN) containing unmethylated CpG motifs are powerful stimulators of innate as well as adaptive immune responses, exerting their activity through the triggering of the endosomally localized TLR9 by a poorly understood mechanism. The immunopotency and broad …
-
DNA chip designed antisense oligodeoxynucleotides targeting EGFR MRNA for brain tumour therapy
… therapies for future treatment of GBM. Oligodeoxynucleotides (ODNs) are small pieces of DNA that are often modified to increase their stability to nucleases and can be targeted to the aberrant gene in order to inhibit it and thus prevent its transcription into protein. By specifically …
-
Immunostimulatory effects and delivery of oligodeoxynucleotides containing CpG motifs (CpG-ODN) in neonatal broiler chickens
Oligodeoxynucleotides containing CpG motifs (CpG-ODN) have been shown to stimulate the innate immune system against a variety of bacterial, viral, and protozoan infections in a variety of vertebrate species. The objectives of this study were to investigate the immunostimulatory effect of CpG-ODN …
-
Incorporation of CpG Oligodeoxynucleotides into α2-Macroglobulin: Development of a Novel Vaccine Adjuvant Delivery Mechanism
… Following cellular uptake, CpG-containing oligodeoxynucleotides (CpG ODN) are trafficked to the endosome where they bind Toll-like receptor 9 (TLR9) to initiate a signaling cascade that culminates in the release of numerous pro-inflammatory cytokines. Because of their immunostimulatory …
-
Biodegradable polymers for the sustained release of antisense nucleic acids
Antisense oligodeoxynucleotides can selectively inhibit individual gene expression provided they remain stable at the target site for a sufficient period of time. Thus, the efficacy of antisense oligodeoxynucleotides may be improved by employing a sustained release delivery system which would …
-
Esterase 2-oligodeoxynucleotide conjugates as enzyme reporter for electrochemical detection of DNA and identification of bacterial species
… steps: immobilization of thiol-modified capture oligodeoxynucleotides onto electrode, recruiting EST2 to electrode vicinity by means of nucleic acids hybridization, and amperometric determination of p-aminophenol produced by EST2 catalytic hydrolysis of p-aminophenylbutyrate. Generally, EST2 …
-
G Protein and Androgen Signaling in Ovarian Function
… In contrast, depletion of XGPR3 using antisense oligodeoxynucleotides enhanced steroid- and gonadotropin-mediated oocyte maturation. Interestingly, collagenase treatment of Xenopus oocytes cleaved and inactivated cell surface XGPR3, which enhanced steroid-triggered oocyte maturation and …
-
A study of DNA-DNA interactions during bacteriophage lambda integrative recombination
… recombination in bacteriophage lambda. Oligodeoxynucleotides 28 bases long containing either the top strand or the bottom strand sequences of the bacterial attachment site (attB) with or without base substitutions in the core region were chemically synthesized. Annealed …
-
Allergen Sensitization and Immunomodulation By Synthetic Ligands, Pul-042, In An Allergen-Induced Asthma Murine Model
… and may be linked to increased mortality. CpG oligodeoxynucleotides (ODNs) is a synthetic ligand that targets Toll-like Receptor (TLR) 9. It has been evaluated as a therapeutic agent for the treatment of cancer, infectious diseases, and for treating allergy and asthma. PUL-042 is also a …
-
Chemical synthesis of DNA and RNA containing thio-substituted bases and their post-synthetic modifications
… example has been the synthesis and study of oligodeoxynucleotides containing 6-thio-2'-deoxyguanine. 6-Thio-2-deoxyguanosine was first discovered as metabolic compound of 6- mercaptopurine (6-MP). Later, it was applied as drug to cure leukaemia. During the research of its toxicity, a method …
-
Trpv1 Channels Contribute to Behavioral Hypersensitivity and Spontaneous Activity In Nociceptors After Spinal Cord Injury
… was induced by intrathecal delivery of oligodeoxynucleotides antisense to TRPV1, which knocked down TRPV1 protein and reduced capsaicin-evoked currents. TRPV1 knockdown also decreased the incidence of spontaneous activity in dissociated nociceptors after SCI. Limited activation of TRPV1 …
-
Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
… lipid particles; SPLP) and cytosine-guanine oligodeoxynucleotides (CpG-ODN; LN CpG-ODN), and examining their ability to act together as a non-viral DNA vaccine in attempt to address the shortcomings of current DNA vaccine approaches. One focus of this thesis concerns investigating the …
-
DEVELOPING STRATEGIES TO CONTROL NECROTIC ENTERITIS IN BROILER CHICKENS
… of cytosine phosphorothioate guanine oligodeoxynucleotides (CpG-ODN) by the in ovo route at day 18 of incubation, and with or without administration of a booster vaccine was able to protect birds against homologous and heterologous CP associated NE in broiler chickens. Immunoprotection …
-
Novel approaches to the synthesis of selenium modified nucleosides, nucleotides and nucleic acids
… phosphoroselenolate triesters, pure oligodeoxynucleotides were isolated using standard deprotection and purification procedures and subsequently characterised by mass spectrometry and circular dichroism. The CD spectra of both modified and native duplexes derived from …
-
Strategies for the induction of mucosal immunity against hepatitis B virus.
… motifs present in the DNA backbone. Synthetic oligodeoxynucleotides (ODN) containing immunostimulatory CpG motifs (CpG) are potent adjuvants for induction of Th1-like systemic immune responses against parenterally-delivered proteins. Herein, the adjuvanticity of CpG ODN relative to or in …
-
Targeting Primary Cilia Immune Receptor Proteins for the Treatment of Polycystic Kidney Disease Mechanisms
… by adding agonists lipopolysaccharide (LPS) and oligodeoxynucleotides (ODN) and antagonist 4-hydroxy chloroquine (HCQ).</p> <p><strong>Results: </strong>From our results we found that the TLR antagonist HCQ increases ciliary length in treated scrambled control, <em>Pkd2</em>knockout (KO) and …
Page 1 of 2