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Showing 1 to 20 of 23 for “"Okadaic acid"”.
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Investigation of the genotoxic potential of the marine biotoxins okadaic acid and azaspiracids
… the genotoxic potential of the marine biotoxins okadaic acid (OA) and azaspiracids (AZAs). Harmful algae blooms (HABs) are an increasing global problem with implications for the ecosystem, economy and human health. Most data available on human intoxication are based on acute toxicity. To date, …
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Tyrosine phosphorylation and cytoskeletal tension regulate the release of fibroblast adhesions
… a localization in small, thin focal adhesions. Okadaic acid, a serine, threonine phosphatase inhibitor, enhances the contracted phenotype, even at low concentrations (0.5mM) of ATP. The localization of focal adhesion components is different in okadaic acid treated cells. In highly contracted …
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Angiotensin Type-2 (At2) Receptor Modulation of Nmda-Mediated Current, Signaling and Apoptosis
… (cPARP), while inhibition of PP2A with 10 nM okadaic acid, significantly increased NMDA-mediated cPARP expression. Further, we demonstrate that the AT2 receptor attenuates NMDA-mediated subunit phosphorylation and current to decrease intracellular Ca2+ levels and subsequent downstream …
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Insulin Receptor Substrate-1 Serine Phosphorylation by a Novel Phosphatidylinositol-3'-Kinase-Associated Serine Kinase Regulates Insulin and Interferon Receptor Signaling
… of PI 3-kinase. pp76 is phosphorylated by an okadaic acid sensitive, PI 3-kinase associated serine kinase (PAS kinase) with biochemical properties that distinguish it from the intrinsic serine kinase activity of PI 3-kinase and evidence suggests that PAS kinase may be pp76. PAS kinase …
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Regulation of Protein Kinases (Syk and PKC zeta) in platelets
… of Thr410 can also be prevented by okadaic acid, a Ser/Thr protein phosphatase inhibitor, at concentrations above 100 nM. However, in PP1c gamma null mice, we did not observe any effect on the dephosphorylation, suggesting that other isoforms of PP1 or other classes of the …
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TNFalpha expression in monocytes/macrophages
… as well as posttranscriptional inhibition. Okadaic acid, a protein phosphatase inhibitor, seems to overcome the posttranscriptional inhibition exerted by dexamethasone when TNF expression is induced. The posttranscriptional regulation of TNF was further studied in human monocytic THP-1 …
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Ecotoxicology of marine biotoxins in bivalve shellfish
… in Irish waters. The effects of the DSP toxin okadaic acid (OA) were investigated on three shellfish species: Mytilus edulis, Ruditapes philippinarum and Crassostrea gigas. Histological examination of the gill, mantle and hepatopancreas tissues revealed varying intensity of damage depending …
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Protein Phosphorylation in Archaea
… entire phosphatase gene was isolated. The amino acid sequence of the phosphatase encoded by this gene displayed greater than 30% identity with eucaryal protein-serine/threonine phosphatase type 1. The gene encoding the Methanosarcina phosphatase was expressed in Escherichia coli. The expressed …
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Identification of a Low Molecular Weight Protein Tyrosine Phosphatase and Its Potential Physiological Substrates in Synechocystis sp. PCC 6803
… PCC 6803, SynPTP, possesses significant amino acid sequence similarity with known low molecular weight protein tyrosine phosphatases (PTPs). To determine the gross functional properties of this hypothetical protein, open reading frame slr0328 was cloned, and its predicted protein product was …
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Studies of tumor promoters and drug-metabolic enzymes in hamster buccal pouch mucosa
… effects of two new mouse skin tumor promoters, okadaic acid (OA) and methyl methanesulfonate (MMS), on HBPM. Short term effects of OA were studied. A single application of10 1.1.g of OA in 0.1 ml of acetone produced marked inflammation as well as an increased mitotic rate (p<0.01) as compared to …
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Protein Phosphatase 4 in human spermatozoa: investigations into location, regulatory subunits and role in motility
… was identified as a possible associate of PPP4c. Okadaic Acid was used to explore the effects on motility of the inhibition of PPP4 and PPP2A in human spermatozoa. The incubation of human spermatozoa with OA at concentrations aimed to inhibit PPP4 and PPP2A did not result in a significant increase …
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Characterisation of a protease-dependent gene regulation system for gene therapy applications
… to cellular stress induced by the cytotoxin okadaic acid in vitro. Following adeno-associated viral vector (AAV)-mediated delivery of a green fluorescent protein (GFP) reporter gene under control of the regulatory system to the rat substantia nigra pars compacta, expression of GFP was induced …
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Identification and characterization of the acid phosphatase from Francisella tularensis and its potential role in pathogenesis
… intermediates of the respiratory burst. Purified acid phosphatases (ACP) from Leishmania donovani and Legionella micdadei have been shown to cause the dose dependent suppression of the respiratory burst (Remaley et al., 1984 and Saha et al., 1985). Depression of the respiratory burst by …
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Design, development, and evaluation of novel polymer-drug conjugates and interpolymer complexes for the treatment of neurodegenerative disease.
… (HEXNAP) was conjugated to polyacrylic acid (N5 and N10). These conjugates were thoroughly characterised, and their antioxidant and cholinesterase inhibitory activities were evaluated. Additionally, in silico molecular modelling was performed to determine cholinesterase inhibition …
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The Role of [beta]2-Syntrophin Phosphorylation in Secretory Granule Exocytosis
… underwent Ca2+-dependent and okadaic acid-sensitive dephosphorylation upon stimulation of insulin secretion. Stimulation-dependent dephosphorylation was confirmed by immunoprecipitation of 32P-labeled GFP-b2-syntrophin.Mass spectrometry of immunoprecipitated GFP-b2-syntrophin …
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Regulation of mTORC1 Signalling and Amino Acid Uptake by Iron Availability in Caco-2 Intestinal Epithelial Cells
… of iron depleted cells with the PP2A inhibitor okadaic acid prevented both the induction of REDD1 and the inhibitory effect it has on mTORC1 signalling. Conversely, treating cells with MG132 (a proteasome inhibitor) enhanced REDD1 protein levels in Caco-2 cells and suppressed mTORC1 …
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Optimisation of high value metabolite production from benthic marine dinoflagellate Prorocentrum lima.
Toxins produced by harmful algal blooms (HABs) are known to pose contamination risks to seafood products (e.g. fish and shellfish) consumed by human. In order to control contamination risks, monitoring regimes have to be performed rigorously. The effort to monitor the amount of toxins in consumable …
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TRAF Regulation of Caspase-2-Dependent Apoptosis in Response to DNA Damage
… that two separate inhibitors, calyculin A and okadaic acid (1uM), were able to promote the dissociation of the MCC. This led to the activation of the APC/C, but the cells remained in mitosis as evidenced by high levels of Cdk1 activity and chromosome condensation. This is the first time that …
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Transcriptional regulation of the human alpha 2(I) procollagen gene
… genistein, and the PP2A phosphatase inhibitor, okadaic acid. Staurosporin also had a significant inhibitory effect on α2(1) procollagen promoter activity in CT-1 cells transfected with the human proximal α2(1) procollagen promoter and on steady state collagen mRNA levels. These results indicate …
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