Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"Oil-Red-O-Staining"”.
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Mechanisms of growth hormone inhibition of adipose tissue growth
… tissue weight and adipocyte size were compared between GH-deficient growth hormone releasing hormone receptor (Ghrhr) homozygous mutant mice (i.e., lit/lit mice), lit/+ mice, and lit/lit mice injected with GH. lit/lit mice had less body weight but more subcutaneous fat and larger adipocytes …
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Exogenous Lipoid Pneumonia in Children: A Systematic Review and Case Series from South Africa
… exogenous lipoid pneumonia (ELP) and elucidate oil administration practices. Methods: A retrospective study of children with histologically-confirmed ELP at Red Cross Children’s Hospital, South Africa. Caregivers were interviewed to understand oil administration practices. Results: Twelve …
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Characterisation of the cell-surface localised palmitoyl acyl transferase, DHHC5
… DHHC5 and again the di-cysteine motif was required for this. Therefore, it was concluded that DHHC20 palmitoylates DHHC5 at a di-cysteine motif on its C-tail, and regulates recruitment of PLM to DHHC5.<br/><br/>A peptide-based array covering the entire DHHC5 C-tail was developed and used to …
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Exploring the influence of adipokines on neuronal function in Alzheimer’s disease
… adipocyte-like characteristics (determined by Oil-Red-O staining). To understand the potential rescue effect of conditioned media from adipocyte-like cells on neuronal-like cells, and commercially available adipokines (chemerin, leptin and resistin) on neuronal-like cells and effect of chemerin …
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Investigating the Effects of Sodium Benzoate on Fat Deposition, Pharyngeal Pumping, and Related Genetic Mechanisms in Caenorhabditis elegans
… can promote lipid accumulation though the use of Oil Red O staining methods. Results from the study concluded that sodium benzoate exposure resulted in a significant difference in staining levels of lipid after 72 hours of dosing. The study also tested different target genes of interest dealing …
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Kryokonservierung von hepatischen Sternzellen der Ratte
… cell proliferation assays, apoptosis testing, Oil Red O-staining, SDS-Page and immunoblotting of different marker proteins for fibrogenesis has been passed through. These examinations showed no significant differences between the cryopreserved and untouched cells. Neither the cell morphology …
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The Isolation and Differentiation of Adipose Derived Mesenchymal Stem Cells
… differentiating adipose-derived MSCs into desired cell types. Specifically, I assessed the capacity of a non-enzymatic sonication based method to the collagenase digestion based method of isolating MSCs from human adipose. Sonication yielded fewer total cells than collagenase. However, alizarin …
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Effect of yerba mate tea caffeine and green coffee by-products on in vitro adipogenesis and lipid accumulation and their incorporation into functional beverages
… of lipid accumulation in 3T3-L1 adipocytes compared to synthetic caffeine (SC). The antioxidant capacity (AC) and total polyphenol content (TPC) of these novel caffeine sources were also investigated and compared to SC using the Oxygen Radical Absorbance (ORAC) assay and the Folin-Ciocalteu …
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Fibromodulin and Dystrophin in Atherosclerosis: Novel roles for extracellular matrix in plaque development
… to be thicker and more heterogeneous, compared to fibrils generated in the control mouse. Murine plaques with an inflammatory phenotype, with a fibromodulin-deficient ECM, were also smaller with decreased lipid accumulation, whereas cell proliferation was increased. In addition, we show, for …
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Discovery of new biomarkers of mild cognitive impairment and Alzheimer's disease risk in buccal cells using laser scanning cytometry
… of Aβ and Tau) and/or DAPI, Fast Green and Oil Red O dyes. In an initial study an LSC protocol was developed to identify and measure differences in buccal cell types and nuclear DNA content as well as a significant increase in micronuclei measured in AD (n=10) and Down’s syndrome (n=10) …
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Modelling the early development of cartilage and bone in Mseleni Joint Disease using induced pluripotent stem cells
… OCT3/4, NANOG and SOX2 and immunocytochemical staining for their proteins. In vitro differentiation into the three germ layers was confirmed by ICC. To generate iPSC-derived mesenchymal stem cells (iMSC) from the iPSCs, two differentiation protocols were tested: one with low glucose medium and …
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Modification of Low Density Lipoprotein and the Effects of Modified Low Density Lipoproteins on the Endocytic and Secretory Repertoire of the Macrophage
… by reagent H<sub>2</sub>O<sub>2</sub>, as measured by the thiobarbituric acid (TBA) assay, than LDL prepared from plasma anticoagulated with citrate (CDP-LDL). The LDLs required 1-3 mM H<sub>2</sub>O<sub>2</sub> for maximal oxidation by this assay, and ED<sub>50</sub>s were 1.7 x 10<sup>-3</sup> …
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Mechanism of Rare Variant In Acta2, P.Arg149Cys, Driving Diverse Vascular Disease
… in <em>ACTA2</em> (smooth muscle (SM) α-actin) predispose to thoracic aortic aneurysms and dissections (TAAD) and early-onset coronary artery disease (CAD). The most common <em>ACTA2</em> mutation is a genetic alteration of arginine 149 to a cysteine, <em>ACTA2</em> p.Arg149Cys, which accounts …
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The effects of novel and synthetic estrogens on liver health and microbiome crosstalk
… between estrogens and the gut microbiome is explored. Pathway preferential estrogen 1 (PaPE-1) is a promising novel estrogen receptor ligand that has been shown to favorably target tissues through selective activity. Though its effects on weight gain and fat accumulation have been previously …
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A study of the protective effects of an aspalathin enriched green rooibos extract on experimentally induced hepatic steatosis
… cell viability, while lipid content was measured using oil red O staining normalized for cell number by crystal violet staining. The effect of GRT extract on mRNA relevant to lipogenic genes (PPAR-α, SREBP, FASN and CPT1), inflammation (TNF-α) and glucose metabolism (GCK and ChREBP) were …