Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 72 for “"Oct4"”.
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Oct4 and Sox2 regulate methylation at the Igf2/H19 imprinting control region
… transgene hypomethylation in F9 cells and that Oct4 was present at the endogenous ICR. In addition, each of the octamers is flanked by a sequence that allowed cooperative binding of Oct4 and Sox2 in vitro. To determine at which developmental stage the octamers regulate ICR methylation, we …
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Mechanisms of Oct4 in the entry to, maintenance of, and exit from pluripotency
… However, the transcription factor Oct4 must be maintained within a tight window of expression; depletion results in extraembryonic differentiation, while overexpression also results in exit from pluripotency. Oct4 was identified as a protein expressed in the early embryo and in germ …
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Hyperosmotic Stress Enzyme Signaling Modulates Oct4, Nanog, And Rex1 Expression And Induces Prioritized Differentiation Of Murine Embryonic Stem Cells
<p>HYPEROSMOTIC STRESS ENZYME SIGNALING MODULATES OCT4, NANOG,</p> <p>AND REX1 EXPRESSION AND INDUCES PRIORITIZED DIFFERENTIATION OF</p> <p>MURINE EMBRYONIC STEM CELLS</p> <p>by</p> <p>JILL SLATER</p> <p>MAY 2013</p> <p>Advisor: Daniel Rappolee, Ph.D.</p> <p>Major: Physiology</p> <p>Degree: Doctor …
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Molecular control of embryonic stem cell identity
… by the key pluripotency transcription factors, Oct4 and Nanog. The data from this study have also been compared and integrated with Oct4 and Nanog DNA binding data obtained in a different study using the ChIP-PET technology. In order to gain further insight into the mechanisms by which the …
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
… sorted according to their levels of Oct4-GFP expression displayed distinctly different expression levels of pluripotency and early differentiation markers and differentiated into cardiomyocytes at different efficiencies. However, when allowed to self-renew after sorting, the …
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Germ cell nuclear factor is not required for the down-regulation of pluripotency markers in fetal ovarian germ cells
… retain expression of the pluripotency markers Oct4 and Nanog longer than any other cells in the body. While somatic cells repress these markers during gastrulation, female germ cells continue to express them until around the time of meiotic initiation. It is not yet clear why pluripotency …
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Δnp63 Regulates A Complex Network of Target Genes In Limb and Epidermal Development
… genes involved in maintaining pluripotency, like Oct4, may be upregulated in the absence of ΔNp63. To test this, q-RT PCR was performed for Oct4 mRNA with wild type and ΔNp63-/- 18.5dpc embryo skin. I found that the level of Oct4 was dramatically increased in the absence of ΔNp63-/-. Based on …
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Optimizing alginate-chitosan microcapsules using co-axial air flow method as 3d stem cell microenvironment
… the proliferation and differentiation of mouse OCT4-GFP embryonic stem cells is investigated. Microcapsules are produced by extrusion of alginate into a calcium chloride gelation bath with the aid of a co-axial air flow. It is shown that the size of the spheres is controlled based on needle …
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USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
… four genes were required to induce pluripotency: Oct4, Sox2, Klf4, and c-Myc (OSKM). Since then, pluripotency has been induced with OSKM most effectively by transfection or transduction. However, both are difficult to control and can have undesirable off-target effects, e.g. random mutations in …
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Studying the principles of cell identity transitions using naïve pluripotency induction as a model
… I reconciled route differences, finding precise Oct4 expression as a unifying, essential and sufficient feature. Different routes achieve this required Oct4 expression by diverse logics, highlighting that successful identity change does not simply require activation of the destination network but …
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Primitive Neural Stem Cells in the Mouse Brain
… whether they express the pluripotency gene Oct4 in the adult brain as the do embryonically. Next, I addressed the cell cycle time of pNSCs and whether they are activated to proliferate to repopulate dNSCs after dNSC and downstream progenitor ablation. Finally, I identified cell type specific …
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Μοριακά δίκτυα δυνητικών stem κυττάρων στο κακόηθες μελάνωμα του δέρματος
… Οι μεταγραφικοί παράγοντες EZH2, SOX2 και Oct4 αποτελούν μόρια – κλειδιά στον έλεγχο του ρυθμιστικού δικτύου του stemness των εμβρυϊκών stem κυττάρων (ESCs). Είναι πλέον γνωστό ότι η χρωματίνη στα ESCs περιλαμβάνει περιοχές με «αντιμαχόμενες» τροποποιήσεις ιστονών (bivalent domain), οι …
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Analysis of combinatorial gene regulation with thermodynamic models
… interaction patterns among three regulators: Oct4, Sox2 and Nanog on ten target genes. We further proposed a computational framework, Network-Identifier, utilizing Interaction-Identifier, to reconstruct gene regulatory networks. Applied to five datasets of differentiating embryonic stem cells, …
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Bone morphogenetic protein (BMP-7) και μοριακά δίκτυα δυνητικών stem κυττάρων στον νεφρό
… ρόλο παίζουν οι μεταγραφικοί παράγοντες Oct4 Sox2 και Nanog, οι πρωτεϊνες της ομάδας Polycomb όπου ανήκει ο EZH2, και microRNAs. Η σηματοδοτική οδός της ΒΜΡ συμμετέχει δραστικά κατά τον επαναπρογραμματισμό ωρίμων σωματικών κυττάρων σε κύτταρα με ιδιότητες stem κυττάρων (iPS). Στον νεφρό, …
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In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
… vector encoding the transcription factors Oct4, Sox2, cMyc, and Klf4 to create a line of naive-like porcine induced pluripotent stem cells (iPSCs). This reprogramming vector was verified first in murine cells, the system in which it was developed. Successful production of both murine and …
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Karyopherin alpha subtypes and porcine early stage embryo development
… BRN2. Octamer-binding transcription factor 4 (OCT4) adopts a nuclear localization in all nuclei prior to the 2-cell stage of development, whereas OCT4 is undetectable in nuclei at the 4-cell stage. <em>In vitro</em> binding assays showed that both BRN2 and OCT4 are able to bind with multiple …
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Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
… conditions. The doubling time (t[sub]d) of R1 Oct4-GFP cell line was found to be 13 hours in LIF⁺ culture and 8 hours in culture with LIF addition after 7 days of LIF withdrawal, implying that cell proliferation rate is higher for cells receiving a sudden upregulation of genes controlling cell …
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Evolutionary, structural and functional features of cellular signalling networks
… of the stem cell transcription factor Oct4. The results presented here facilitated characterisation of a novel post-translational modifier of Oct4, the glycosyltransferase Ogt. Chapter 5 highlights my key findings from applying evolutionary and data integration approaches to signalling …
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Conversion of equine umbilical cord matrix mesenchymal stem cells to the trophectoderm lineage using the Yamanaka reprogramming factors
… the use of the Yamanaka transcription factors, OCT4, SOX2, KLF4, and MYC (OSKM). Advanced transgene delivery methods utilizing non-integrating viruses for transduction of target cells has provided new opportunities for regenerative medicine in humans and other species. We sought to use this …
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Reprogramming cellular fate using defined factors
… forced expression of four transcription factors, Oct4, Sox2, c-Myc and Klf4 can induce a pluripotent state in somatic cells, without the need for embryo destruction. The work presented here aims to characterize reprogramming using defined factors and provide insight into the mechanisms governing …
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