Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"Occludin"”.
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Role of Occludin in the Regulation of Epithelial Tight Junctions
… gastrointestinal, pulmonary and renal diseases. Occludin is the major transmembrane protein of TJ, a deletion of which leads to a complex phenotype including chronic inflammation in several epithelial tissues of occludin deficient mice and poor TJ integrity in epithelial cell lines. Its down …
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Acetaldehyde and Epithelial Junctions: Role of Pp2A and Occludin Phosphorylation
… were used to track the localization of occludin in the cell lines tested, in cells cultured in monolayers or in a three-dimensional model in Matrigel. Results for acute administration of acetaldehyde were confirmed in mouse ileum.</p> <p>Acute administration of acetaldehyde was …
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Role of Protein Kinase C Zeta (PKCζ) in Regulation of Epithelial Tight Junctions
<p>Occludin is hyperphosphorylated on Ser and Thr residues in intact epithelial tight junctions. The dynamics of epithelial tight junctions appear to involve reversible phosphorylation of occludin on Ser and Thr residues. In the present study we determined the role of PKCζ in occludin …
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Effects of fructan source and degree of polymerization on intestinal barrier function and histomorphology characteristics in obese C57BL/6 mice
… in mice fed inulin compared to other treatments. Occludin mRNA abundance was lowest (p<0.05) in fructan sources compared to cellulose. When comparing 5% vs. 10% cellulose treatments using contrasts, 10% cellulose led to greater (p<0.05) intestinal crypt depth in all intestinal regions, except the …
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Role of Extracellular Signal-Regulated Kinase (ERK) in Regulation of Intestinal Epithelial Tight Junctions
… shown that EGF increases Thr-phosphorylation of occludin by a MAP kinase-dependent mechanism. This study aimed at looking at the role of ERK in regulation of tight junctions using pharmacological and molecular techniques.</p> <p><strong>Hypothesis:</strong> ERK protects tight junctions in …
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The effect of oral glutamine supplementation on gut permeability and heat shock protein regulation in runners with a history of gastrointestinal distress
… of the heat shock response resulting in occludin stabilization, and (2) depresses the exercise induced inflammatory response. Methods. Eight human subjects (n=8) participated in baseline (PRE) testing, a glutamine (GLN), and placebo (PLA) supplementation trial in a double blind design. …
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Hemodynamic regulation of blood-brain barrier phenotype: A cooperative effort linking adherens and tight junction complexes
… tight junction (TJ) proteins (e.g. VE-Cadherin, occludin, ZO-1, claudin-5) jointly consolidate the apical surface of adjacent brain microvascular endothelial cells (BBMvEC’s) creating the blood-brain barrier (BBB), a critical regulator of CNS homeostasis. Junctional disassembly and barrier …
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Einfluss des Flavonoids Quercetin auf die epitheliale Barrierefunktion der humanen Kolonkarzinom-Zelllinie Caco-2
… Junction (TJ) insbesondere durch die Proteine Occludin und die Claudine definiert. Ziel dieser Arbeit war es, die Effekte des am häufigsten vorkommenden Flavonoids Quercetin auf die Barrierefunktion der Kolonkarzinom-Zelllinie Caco-2 zu untersuchen. Hierbei zeigte sich, dass Quercetin …
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Effect of Diabetes Mellitus on Ocular Surface Tight Junctions and Glycocalyx
… claudin-2, claudin-3, ZO-1, ZO-2, ZO-3, and occludin was quantified using real time PCR and western blotting. Type I diabetes was induced in mice by streptozotocin injection. Phenol red thread test, fluorescein staining and wheat-germ agglutinin corneal staining and confocal microscopy of …
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Schlussleisten (Zonulae occludentes) und biochemisch verwandte Strukturen: Tight Junction-Proteine und -Funktionen in mehrschichtigen Epithelien, mit besonderer Berücksichtigung der Plattenepithelmetaplasie
… Stellen, an denen typische TJ-Proteine wie z.B. Occludin oder bestimmte Claudine angereichert waren. Diese neuartigen TJ-Protein-Strukturen wurden daher als „Stud Junctions“ (Puncta occludentia) bezeichnet und mit anderen TJ-verwandten, Claudin- bzw. Occludin-haltigen interdesmosomalen Strukturen …
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Caracterização da microbiota intestinal, perfil inflamatório e integridade da barreira gastrointestinal de crianças diagnosticadas com transtorno do espectro autista
… abundance analysis. Zonulin, Claudin, and Occludin concentrations were analyzed from fecal samples. The Children's Eating Behavior Questionnaire (CEBQ) was used to assess eating behavior, while a 24-hour dietary recall (R24h) was employed to evaluate the level of processing and nutritional …
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hiPSC-Derived Cerebral Small Vessel Disease Models
… induced tight junction abnormalities with Occludin discontinuity and Claudin-5 accumulation in hiPSCs-derived Brain Microvascular Endothelial Cells (iBMECs), which resulted in decreased TEER levels and increased permeability. They also induced apoptosis, migration defects, and transcriptome …
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Placental co-culture model reveals Listeria monocytogenes utilization of monocytes for vertical transmission
… monocytes revealed severe disruption of occludin and claudin-1 tight junctions. This study demonstrates Listeria usage of the Trojan horse mechanism to reach and translocate the human placental barrier. Thus, Listeria infection can utilize both extracellular and intracellular forms of the …
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Defining the mechanism(s) of Hepatitis C virus (HCV) entry
… the tight junction (TJ) proteins Claudin-1 and Occludin. Primary HCV strains cannot be efficiently cultured in vitro, making the evaluation of potential antiviral compounds in a biologically relevant context extremely difficult. Despite being suitable for high-throughput screening, most …
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Charakterisierung der Funktion des Tight Junction Proteins hu-CLDN1 und seine Bedeutung bei der Tumorgenese
… Zusammenhang, daß in Brusttumorzellen, die weder Occludin noch ZO-1 exprimieren (MDA-MB-435) die physiologisch korrekte Expression von hu-CLDN1 existiert. Offensichtlich ist die Lokalisation von hu-CLDN1 von beiden Proteinen unabhängig. Die Analyse von unterschiedlichen hu-CLDN1 positiven und …
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The Effects of Blast-Induced Neurotrauma on Blood-Brain Barrier Permeability, Cell-Cell Junction Protein Levels and the Role of Peroxynitrite
… the presence of the tight junction protein, occludin, in cerebral blood vessels, causes deficits in sensorimotor function and spatial learning and working-memory, and increases the formation of potent oxidant, peroxynitrite. A better understanding of the direct and indirect effects of blast …
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Reciprocal communication between cancer cell and tumor microenvironment promotes breast cancer metastasis
… cells and is capable of degrading Claudin and Occludin that are key components of BBB. Knockdown of MMP1 in brain metastatic cells significantly suppressed their ability of brain metastasis in vivo, while ectopic expression of MMP1 significantly increased the brain metastatic ability of breast …
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Molecular Analysis of Hepatitis C Virus Envelope Glycoprotein E2 Binding and Entry
… incorporation of claudin-1 receptor (CLDN-1) and occludin receptor (OCLN) (indirect interaction) has been reported. Then, fusion process is intiated between viral and cellular membranes following acidification of endosomes. All known HCV-receptor interactions are mediated by HCV envelope protein 2 …
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Effect of the Insulin-like Growth Factor (IGF) Axis on the Transport Properties of Endothelial and Epithelial Cells In Vitro
… junction components E-cadherin, b-catenin, and occludin. The differences in parental and IGF-I secreting cells was not due to extracellular stimuli since inclusion of IGF-I, IGFBP-3, or co-culture with SV40-IGF-I cells did not alter the barrier properties of parental cells, suggesting that …
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