Global ETD Search
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Showing 1 to 20 of 22 for “"OPA1"”.
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TAp73 regulates mitochondrial dynamics through an OPA1 axis
… a new molecular mechanism involving TAp73/OPA1 that controls mitochondrial morphology (Buckley et al., 2020). OPA1 drives fusion of the inner mitochondrial membrane and controls cristae remodelling, a process facilitating the execution of apoptosis. I have shown that TAp73 regulates OPA1 …
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The Mitochondrial Fusion Protein, Optic Atrophy 1 (OPA1), Alters Cellular Metabolism to Support Cancer Cell Viability
… The inner mitochondrial membrane GTPase, OPA1, is a cristae-organizing protein that directly organizes formation of the electron transport chain for oxidative phosphorylation and thereby further coordinates with the tricarboxylic acid (TCA) cycle. To study mitochondrial fusion as a target, …
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KUPFFER CELLS¿ MITOCHONDRIAL PLASTICITY AFFECTS SYSTEMIC IMMUNO-METABOLISM
… proteins that regulate their dynamism, OPA1 is the gatekeeper of mitochondrial fusion and thus modulates oxidative phosphorylation. AIM: The immunomodulatory role of mitochondria on the activity of antigen-presenting cells has been proposed and needs further investigation. This is a …
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Mitochondrial Stress Signals Induce a Drug Tolerant Persister Phenotype in Triple Negative Breast Cancer Cell Models
… short isoforms of mitochondrial fusion protein OPA1 and transient knockdown of OPA1 also resulted in a reduction in mtDNA encoded genes. Furthermore, CRISPR-Cas9 mediated knockout of OPA1 in HeLa cells significantly reduced mtDNA abundance, confirming that OPA1 is required to maintain mtDNA …
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Sex Differences in Cancer Cachexia and a Novel Mitochondrial Target for Cancer-Induced Muscle Wasting
… mitochondrial fusion protein, Optic atrophy 1 (OPA1) which plays a significant role in skeletal muscle health, is suppressed in the development of CC. Furthermore, most pre-clinical CC studies were mainly focused on males although there are distinct phenotypical differences in skeletal muscle …
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Role and regulation of mitochondrial permeability transition in cell death
… release of the profusion GTPase Optic Atrophy 1 (OPA1), which controls CJ integrity, and cytochrome c leading to apoptosis. Interference RNA knockdown of Bax blocked OPA1 and cytochrome c release after THG treatment, but did not prevent the MPT showing that Bax was essential for cytochrome c …
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Insights into the Functional Link between MtHsp40:MtHsp70 Network and Mitochondrial Homeostasis
… in fragmented mitochondria. In addition, OPA1 short-isoform highly accumulated in fragmented mitochondria, suggesting that ratio between mtHsp40 and mtHsp70 is a determinant of OPA1 cleavage, which in turn determines mitochondrial morphology. Imbalance between mtHsp40 and mtHsp70 led to …
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Autism Spectrum Disorder and Mitochondrial Dysfunction: The Role of Mitochondrial Dynamics
… of six essential genes (DRP1, FIS1, MFN1, MFN2, OPA1, STOML2) involved in mitochondrial fusion and fission were examined using targeted next-generation bisulfite sequencing (tNGBS) between ASD and control participants in a South African cohort. Significance for all experiments was determined …
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Mitochondrial dysfunction in hereditary optic neuropathies
… linked to mutations in nuclear gene encoding Opa1, a dynamin-related protein localized in the mitochondrial matrix. Although the clinical course is slightly different, the endpoint of ADOA is exactly the same of LHON: optic nerve degeneration with specific involvement of retinal ganglion …
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Disassembly of electron transport chain complexes drives macrophage TLR responses by reprogramming metabolism and translation
… of OxPhos via myeloid-specific knockout of OPA1, which stimulates ETC complex assembly, exacerbates sepsis in mice while inhibition of mTORC1 reverses this effect. These findings reveal that disassembly of ETC complexes underlies macrophage metabolic switch and inflammatory responses and may …
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An investigation into the novel effects of melatonin treatment on differentiated C2C12 myotubes in the absence and presence of pathology
… for future apoptotic events, while decreasing Opa1 protein expression. For paper 3, Pre-treatment with MEL (after excess UA as a pathological stressor) increased routine respiration (to above normal levels), leak control ratio, complex-1 and 2 linked OXPHOS, ETS capacity, the contribution of …
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Regulation and roles of nutrient-dependent mitochondrial fusion
… fusion required the regulators, Optic atrophy 1 (Opa1) and Mitofusin 1 (Mfn1).;Metabolomic analysis revealed both QLR-dependent and fusion-dependent changes in levels of metabolites involved in the urea cycle, Krebs cycle and REDOX balance. Findings in this project suggest putative mechanisms …
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Investigating the effects of Wnt/β-catenin Signalling on Melanoma Cell Metabolism and Mitochondrial Dynamics
… and fission proteins including MFN1, MFN2, OPA1 and DNM1L suggest that activation of Wnt/β-catenin signalling can increase mitochondrial fusion and decrease mitochondrial fission in melanoma cells. Cellular metabolic analysis using the Seahorse Bioscience XFe96 Analyzer suggests that …
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Characterisation of new factors regulating mitochondrial dynamics and cellular metabolism
… accompanied by an increase of the fusion factor OPA1, and a reduction of phosphorylated mitochondrial fission factor (pMFF), a well characterised fission factor. Furthermore, cells lacking MTFR1L harboured reduced AMPK activation and consequently dampened signalling upon electron transport chain …
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Regulation of Mitochondrial Morphology by ERK1/2-Mediated Control of Mfn2 Stability
… Mitofusin 2(Mfn2), Optic atrophy protein 1(Opa1), Dynamin related protein 1(Drp1), Fission protein 1(Fis1) 등의 미토콘드리아 형태조절 단백질(mitochondria-shaping protein)이 관여하는 미토콘드리아 융합-분열 기구(mitochondrial fusion-fission machinery)에 의해 정교하게 조절되고 있다. 이러한 형태조절 단백질들이 어떻게 분자 수준에서 조절 되어 그들의 역할을 수행하는지에 관해서는 아직 …
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A study on the role of oxidative stress and protein kinase signalling in hyperglycaemia induced cardiac remodelling
… fusion regulatory protein, optic atrophy-1 (OPA1), with the inhibition of p38MAPK in high glucose attenuating this effect. Conclusion Hyperglycaemia induced pyknotic-like phenomenon, suppressed the proliferation, and reduced mitochondrial fusion protein machinery of mESC-derived cardiac-like …
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Il sistema dell'Eme Ossigenasi-1 (HO-1) nel Glioblastoma Multiforme
… II, COX IV, Ndufa6, ATP sintetasi) e dinamica (OPA1, MNF2 , MNF1, FIS1). Inoltre, la traslocazione nucleare di Nrf2 è stata valutata mediante analisi immunocitochimica. Nel loro insieme, i nostri dati suggeriscono che HO-1 e il suo sottoprodotto CO mostrano un effetto specifico sulle cellule su …
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Untersuchungen zu den molekularen Ursachen der X-gebundenen juvenilen Retinoschisis - vom Gendefekt zum Mausmodell
… der autosomal dominanten Optikusatrophie (OPA1). Auch hier konnte mit Hilfe von Mutationsanalysen ein Ausschluß des Gens erfolgen. Die X-gebundene juvenile Retinoschisis ist eine häufige Ursache juveniler Makula-degenerationen und betrifft etwa 300.000 junge Männer weltweit. …
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Mitochondrial mechanisms in autism spectrum disorder: characterizing the neurotoxic effects of propionic acid in vitro
… fission (DRP1) and fusion (STOML2, MFN1/2, OPA1). Confocal microscopy demonstrated associated disruptions to mitochondrial network morphology and integrity, characterised by decreased mitochondrial volume, increased mitochondrial fragmentation and perturbations to mitophagic flux. Finally, …
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Genotype and phenotype in mitochondrial disorders
… diagnoses were SPG7, dominant optic atrophy (OPA1) and autosomal recessive POLG. We have linked to Hospital Episode Statistics, death certificates and the cancer registry. We present survival analysis, causes of death and cancer standardised incidence ratios. Treatment <br>Mitochondrial …
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