Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"OGT"”.
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Understanding tetratricopeptide repeats of <i>O</i>-GlcNAc transferase
… this modification: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Intriguingly, OGT has also recently been shown to have a secondary function, catalysing the proteolysis of the transcriptional regulator protein host cell factor-1 (HCF-1). OGT contains an N-terminal tetratricopeptide …
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O-GlcNAcylation: A key regulator of hepatic proliferation and differentiation
… regulated by two enzymes, O-GlcNAc transferase (OGT), which adds a glucosamine sugar on serine or threonine residues of proteins, and O-GlcNAcase (OGA), which removes the sugar molecule. The process of O-GlcNAcylation plays a role in regulating the cellular fate of various cell types, including …
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… antagonistic enzymes. The O-GlcNAc transferase (OGT), which adds the sugar onto protein substrates and the O-GlcNAc hydrolase (OGA), which removes it. Mass spectrometric analyses have shown that O-GlcNAc can be found on proteins involved in nearly all biological processes, including trafficking, …
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Gut Microbiota-Generated Trimethylamine N-Oxide and Cardiometabolic Health in Humans
… dilation (FMD) and oral glucose tolerance (OGT) were measured. In study 3, healthy sedentary individuals consumed 4-wks of CHOL and PLC. Fasted blood samples were collected, FMD and OGT were measured. We hypothesized acute and 4-wk choline supplementation would impair FMD and OGT. In study …
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Untersuchungen zur Rolle des HIV-1-Tat-Proteins in der AIDS-assoziierten Vaskulopathie
… wurde O-linked N-Acetylglucosamine-transferase (OGT) als Gen identifiziert, dessen Expression durch HIV-1-Tat unterdrückt wird. Bisher ist bekannt, dass OGT ein Repressor der basalen Transkription und der SP-1-regulierten Transkription ist. Die Expression von OGT wurde sowohl auf mRNA-Ebene als …
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Reductionist animal models to probe protein O-GlcNAcylation
… is carried out by O-GlcNAc transferase (OGT), and its removal by O-GlcNAc hydrolase (O-GlcNAcase or OGA). A range of proteomics studies have suggested that over a thousand intracellular proteins are O-GlcNAc modified. However, little is known of the role of O-GlcNAc on these proteins. In …
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O-GlcNAc-Mediated Protein-Protein Interactions in Cell Signaling
… general. </p><p>Despite the extensive number of OGT and OGA substrates identified, including IFs, the substrate specificity of both of these enzymes remains an open question due to the lack of consensus sequence among O-GlcNAc modified proteins. A prevailing general model is that OGT binds to …
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Signaling pathways regulating skeletal muscle metabolism and growth
… we ablated skeletal muscle O-GlcNAc transferase (OGT), and thus O-GlcNAcylation, and found the knockout mice exhibited enhanced glucose uptake, insulin sensitivity, and resistance to high-fat diet induced obesity. Additionally, mKO mice had a 3-fold increase in circulating levels of interleukin-15 …
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Investigating the role of UDP-<i>N</i>-acetylhexosamine pyrophosphorylases in regulating <i>O</i>-GlcNAcylation in human disease
… a single pair of enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). O-GlcNAcylation has been found to modulate enzyme catalytic activity, protein stability, protein–protein interaction and protein subcellular localisation, and these could lead to regulation of cell proliferation and …
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The regulation of genes of unknown function implicated in nitrosative stress tolerance in Escherichia coli K-12
… \(coli\) K-12 promoters, \(pyeaR\) and \(pogt\), during anaerobic growth. These promoters were identified from transcriptomic studies as being positively regulated by NarL independently of FNR, the master regulator of anaerobic respiration. Biochemical and genetic analyses presented in this …
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Architecture of Escherichia coli promoters that respond to reactive nitrogen species
… examined the regulation of two genes, hcp and ogt, that are reported to be involved in protection from the mutagenic effects of reactive nitrogen species in Escherichia coli. Biochemical techniques were used to investigate promoter activity and the effects of the transcription factors that are …
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Regulating the stabilities of NOD1 and NOD2, two innate immune receptors, with sugars and chaperones
… by the enzyme O-GlcNAc transferase (OGT). A cellular thermal shift assay (CETSA) typically used to detect protein-ligand binding was adapted in order to monitor the stability changes caused by O-GlcNAcylation, and it was found that wild type NOD1 and NOD2 were stabilized when modified …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
… regulated by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAc hydrolase (OGA). While it is elusive how O-GlcNAc alters protein function, altered O-GlcNAc levels are associated with human diseases. To gain insight into the functional consequences of O-GlcNAc-ylation, we reported a method to …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… catalysed by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), that add and remove the sugar moiety, respectively. Ogt gene knock-out is embryonic lethal in a range of animal models, hampering the study of the biological role of O-GlcNAc. O-GlcNAcylation of nuclear and cytoplasmic …
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Small molecule inhibitors against enteric bacterial glycosyltransferases
… O-linked N-acetylglucosaminyltransferase (OGT). As the deletion of gene nleB significantly attenuates C. rodentium virulence, we aimed to find small molecular inhibitors which specifically bind to NleB. Therefore, we performed high-throughput screening (HTS) assays to identify NleB/SseK …
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Computational structure analysis and prediction of Ser/Thr modified by O-GlcNAc in human proteins
… biology approach. The O-GlcNAc transferase (OGT), the enzyme that catalyses the protein O-GlcNAcylation, targets specific Serines and Threonines (S/T) of intracellular proteins. However, while other post-translationally modified residues, including phosphorylated ones, occur within sites …
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The Effects of a 5-Day High-Fat Diet on Skeletal Muscle O-GlcNAcylation
… protein concentrations of O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in whole-homogenates and isolated mitochondria from skeletal muscle. Results were analyzed using independent, two-tailed t-tests and 2-way ANOVA analysis with repeated measures and Bonferroni corrections; a p-value was set …
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FEARCF: Graph-based software library for multidimensional free energy simulations
… including: water, glucose, and the GlcNAc/OGT enzyme-substrate complex.
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Ground Station Mixed-Signal PCB and SFP Ethernet-to-Optical Connector for the Deployable Optical Receiver Aperture (DORA) CubeSat
… direction. The DORA optical ground terminal (OGT) will be stationed on the ground to communicate with the DORA CubeSat in flight. This thesis project will address the ground station analog and digital electronics and data transfer needed to deliver data received from the DORA CubeSat to its …
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Genetic encoding of a stable <i>O</i>-GlcNAc analogue
… by a unique <i>O</i>-GlcNAc transferase (OGT) and removed by an <i>O</i>-GlcNAc hydrolase (OGA). Despite the existence of a single writer/eraser pair, proteomic studies have identified over 4000 O-GlcNAcylated proteins in the nucleus, cytoplasm and mitochondria of various organisms, …
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