Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"OB-fold"”.
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Cell cycle-dependent modification of Pot1 and its effects on telomere function
… region of its most amino-terminal-proximal OB fold, suggesting that this regulation of telomere function may be widely conserved. Mutation of Pot1 phosphorylation sites leads to telomerase independent telomere maintenance through constant HR, as well as a dependence of telomere maintenance …
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Mechanism of Mcm10 function during DNA replication
… binds a conserved motif located between the OB-fold and A subdomain of Mcm2. Although buried in the interface between these domains in Mcm2-7 structures, mutations predicted to separate the domains and expose this motif restore growth to conditional-lethal MCM10 mutant cells. In addition to …
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Engineering and characterisation of anti-progesterone OBodies
… industries. More recently, novel non-immunoglobulin binding proteins, have been similarly engineered to produce high affinity binding proteins that can potentially replace the binding function(s) of antibodies or even surpass them. The OB-fold is a high affinity binding protein domain which …
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Repair eXtreme - DNA repair proteins from Antarctic extremophiles
… systems may offer valuable insights into microbial survival in extreme environments or even extra-terrestrial settings. To investigate this, DNA repair enzymes were identified from Antarctic DV microbial metagenomes using in silico analysis and in vivo characterisation of recombinantly …
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Molecular, Biochemical, and Evolutionary Analysis of Single-Stranded Dna-Binding Proteins From the Mesophilic Archaeon Methanosarcina Acetivorans
… and RPAs contain ssDNA-binding domains with a fold reminiscent of an oligonucleotide/oligosaccharide binding (OB) fold. In the Euryarchaeota, one of the subdomains in Archaea, RPAs display an unusual diversity. Single, multiple, and complex form RPAs have been described in Euryarchaeota. …
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The Roles of the Mcm2-7 Tails in Replication Initiation
… subunit contains an AAA+ ATPase domain, a large OB-fold domain, and extensions of varying lengths on each terminus. Importantly, Mcm2, Mcm4, and Mcm6 contain long unstructured N-terminal tails, which are unrelated to each other and whose role in replication initiation is not well understood. In …
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53BP1 and double-strand break repair pathway choice in cancer
… supporting NHEJ activity. We identified three OB-fold domains in SHLD2/FAM35A, that bind single-stranded DNA and are necessary for NHEJ. Loss of SHLD1 or SHLD2 (SHLD1/2) caused a hyper-resection phenotype, and which, in the absence of BRCA1 was associated with rescuing RAD51 loading. …
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Characterization of a single TRAM domain RNA-binding protein from the Antarctic methanogen Methanococcoides burtonii
… proteins with mild urea to partially unfold the protein, eluting the protein across a NaCl gradient, and refolding it by dialysis. Purification of recombinant RNA-free proteins allowed thorough assessment of the structure-function-stability relationship of Ctr3. Ctr3 unfolded reversibly …