Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"O-glycosylation"”.
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Synthesis Of Oligosaccharide Mimetics By The Desulfurative Rearrangement Of Allylic Disulfides And Stereoelectronic Influence Of C-O Bonds On C- And O-Glycosylation
… effects of proximal C-O bonds in C- and O-glycosylation reactions.</p> <p>The first chapter provides a brief overview of the important roles that oligosaccharides and glycoconjugates play in biological processes and sets out the major advances that have been made in the past few decades in …
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Mutations of the glucose-pts in sublancin-resistant B. subtilis 168 ΔSPΒ
… peptides in bacteria and of cysteine S-glycosylations in general. S-glycosylation of cysteine has previously been shown to result in a metabolically more stable conjugation than the more common O-glycosylation of serine. This stabilization is supported by the high stability of sublancin …
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Biochemical characterization of Cj 1123 c, a putative acetyltransferase & Cj 1319, a putative C6 dehydratase from Campylobacter jejuni.
… jejuni. The enzymes involved in protein glycosylation could provide new therapeutic targets. Two such proteins are Cjll23c, a putative acetyltransferase and Cj 1319, a putative GDP-mannose dehydratase encoded by the N- and O-linked protein glycosylation loci in C. jejuni, respectively. We …
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The isolation, purification, tissue localization and identification of a glycoprotein found in the crude mucus gel of patients with carcinoma of the stomach
… serine and threonine being potential sites for O-glycosylation. In contrast to the glycosylated region is the 'naked' region rich in cysteine and susceptible to proteolysis. The cysteine residues enable mucin monomers to form polymers by disulphide bridges. Alterations of mucin expression take …
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Crystallisation and crystal forms of carbohydrate derivatives
… synthesis of glucopyranoside derivatives by O-glycosylation reactions and displays crystal structures, including a number of previously unsolved acetate protected and deprotected crystal structures. More complex glycoside derivatives were also researched in an aim to study the resultant …
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Síntesis y análisis conformacional de glicopéptidos que incorporan ß-glucosa y a-N-acetilgalactosamina
… work, we studied the consensus sequence for ??O?glycosylation with glucose. For this purpose, two peptides and one glycopeptide were synthesized. As a first approaching, we calculated the spatial distribution of the hydroxyl group of serine, that later will be glycosylated, and interestingly it …
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Beta-o-glicosilación de péptidos que incorporan aminoácidos no naturales
… achieved. It is worth noting that the beta-O-glycosylation has a remarkable and completely different effect on the backbone of the peptides derived from natural and non-natural amino acids, respectively. To go one step further, the synthesis of a glycopeptide incorporating the consensus …
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Expression of the repressor element-1 silencing transcription factor (REST) is regulated by IGF-I and PKC in human neuroblastoma cells
… of neuroblastoma cells. Finally, the glycosylation pattern of the REST protein was analysed, moving from the observation that the molecular weight calculated on REST sequence is about 116 kDa but using western blotting this transcription factor appears to have distinct apparent …
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Cellular Trafficking and Processing Pathways of the Alzheimer β/A4 Amyloid Precursor Protein
… (BFA) prevented normal APP maturation (N- and O-glycosylation and tyrosine sulfation) and abolished APP secretion. Phorbol ester produced a several-fold increase in APP secretion, indicating that protein phosphorylation regulates intra-β/A4 amyloid cleavage and APP secretion. The lysosomotropic …
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Dietary carbohydrates influence the structure and function of the intestinal alpha-glucosidases
… weight is a consequence of additional N- and O-glycosylation of the enzyme. In comparison to the control, the HMW SI was found to be more associated with lipid rafts at the membrane surface which was related to the higher apical sorting of these species. Thus, maltose sensing of small intestinal …
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Characterization of the Human Adenovirus 19a E3/49K Protein
… mass of 80-100 kDa. 12-13 out of 14 predicted N-glycosylation sites are utilized, and there is also evidence for O-glycosylation. 49K contains intramolecular disulfide bonds and is proteolytically processed. As a result of the cleavage the N-terminal ectodomain is secreted: Ad19a E3/49K is the …
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Cloning and expression of a functionally active truncated N-glycosylated KSHV complement regulatory protein and immunohistochemical studies with the anti-KCP peptide antibody
… g1ycosy1ation since there are potential N-and O-glycosylation sites in the protein's primary sequence. Also, diffused bands were obtained in all the electrophoretic gels and Western blots carried out, which is characteristic of glycoproteins. Furthermore, the antibody recognized several larger …
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A Study of the Therapeutic Potential of AF4 Mimetic Peptides
… modifications shows the absence of O-glycosylation and monoubiqutination. Interestingly, multiple phosphorylation sites and an acetylation site of AF9 were identified using mass spectroscopy.</p><p>Our studies on the AF4 mimetic peptide, suggest that inhibition of the AF4-AF9 …
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REGULATION OF GLYCOSYLATION IN MAMMALIAN CELL LINES: INSIGHTS FROM STRUCTURAL GLYCOMICS, ANALYSIS OF GLYCOSYLATION-RELATED GENES AND FUNCTIONAL RNAI SCREENS
… to elucidate the regulation mechanism of glycosylation in mammalian cell lines. First, N-linked and O-linked glycan structures of five commonly used mammalian cell lines, namely CHO-K1, BHK-21, HEK293, COS-7 and 3T3, were characterized by a combination of mass spectrometric and …
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PROTEIN PURIFICATION FROM GASTROPODS
… different species of snails and to study the glycosylation pattern of their tissues by using lectins as glycoprotein-specific antibodies. Purification work started with the screening of 7 different exoglycosidases (α-fucosidase, β-xylosidase, α-mannosidase, β-mannosidase, α-glucosidase, …
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Investigation of the phenotypic and genotypic determinants of disease susceptibility and progression in Crohn’s Disease
… (GALNT2). This enzyme is involved in O-glycosylation, important in the post translational modification of mucins. A GALNT2 genotype/phenotype analysis on the Edinburgh IBD population was completed, with the Dundee IBD population used as a replication cohort. In the Edinburgh IBD …
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Detailed Characterization of Structure-Activity Relationships in the Human Adiponectin Molecule
… The structural pattern of the threonine O-glycosylation in the NH2-terminal region was further characterized by quantitative protein-level analysis of the distinctive glycosylation patterns of the peptide adiponectin-(19-39) isolated from different sitedirected mutant forms. These studies …