Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"O-Glycans"”.
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Analyzing the Role of Mucin O-Glycans in Regulating Microbial Virulence
… by investigating how mucins and their associated glycans influence microbial physiology in the opportunistic fungal pathogen, Candida albicans, and the gram-negative bacterial pathogen, Vibrio cholerae. First, I purify and characterize libraries of mucin-attached glycans and establish their role …
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Glycosylation of Prostate Cancer Cell Lines and Glycosyltransferase Characterization and Inhibition
… of human prostate cell lines. Cell surface glycans were analyzed using lectins and antibodies. Glycans were also released from glycoproteins, purified and analyzed by tandem mass spectrometry. The enzymatic activities and expression levels of glycosyltransferases responsible for the …
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The regulation of bacterial virulence by mucin glycans
… that forms the mucus gel. Upon sensing the O-glycans displayed mucin, opportunistic pathogens can alter their gene expression, and are less likely to engage in virulence behaviors like biofilm formation and toxin production. Therefore, mucin gels can be considered a sophisticated bioactive …
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The biochemical analysis of mucus and mucins in respiratory diseases with a focus on tuberculosis
… analysis showed that the majority of the O-glycans detected were sialylated and that core 3 and 4 O-glycan structures diminished in the presence of HIV.
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Development of coupled enzyme assay and in vitro synthetic biology approach for glycosylation pathway characterization
Glycans play essential roles in living organisms that encompass a wide range of biological functions ranging from energy metabolism all the way through to intricate cell signalling pathways. They carry out these functions either directly by participating in metabolism and binding or indirectly by …
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Characterization of Glycans Derived from Biological Samples Using LC-MS/MS
… types of cancer. Given the essential nature of glycans in crucial biological functions and their association with various health conditions when their expressions are altered, glycomics are becoming leading areas in the development of biomarkers. These biomarkers present unique opportunities to …
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Energetic and dynamic characterization of the IgA1:FcαRI interaction reveals long-range conformational changes in IgA1 upon receptor binding
… terminal GalNAc moieties on IgA1 hinge O-glycans, to the IgA1 hinge in the presence and absence of FcαRI. FcαRI binding to the Fc domain of IgA1 increased the affinity of HAA for the IgA1 hinge, conclusively showing that long-range conformational changes are communicated from Fc domain to …
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Dissection of phloem transport in cucurbitaceae by metabolomic analysis
… results are summarized as below: 1) O-linked glycans in C.maxima were structurally identified as beta-1,3 linked glucose polymers, and the composition of glycans in cucurbits was found to be species-specific. Inter-species grafting experiments proved that these glycans are phloem mobile and …
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IDENTIFICATION OF THE ROLE OF [METHYL]GLUCURONIC ACID ON ARABINOGALACTAN POLYSACCHARIDES IN ARABIDOPSIS THALIANA
Arabinogalactan proteins (AGPs) are proteoglycans heavily substituted by arabinogalactan polysaccharides. These are composed of arabinose and galactose, and minor sugars such as glucuronic acid (GlcA), fucose and xylose. The arabinogalactan polysaccharides do not decorate classical AGPs …