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Showing 1 to 18 of 18 for “"O-GlcNAcylation"”.
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Reductionist animal models to probe protein O-GlcNAcylation
Protein O-GlcNAcylation is a reversible type of glycosylation of serine and threonine residues of nucleocytoplasmic proteins occurring in all animals examined to date. Its installation on protein substrates is carried out by O-GlcNAc transferase (OGT), and its removal by O-GlcNAc hydrolase …
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O-GlcNAcylation: A key regulator of hepatic proliferation and differentiation
O-GlcNAcylation is a posttranslational modification involved in various physiological processes, including development, regeneration, inflammation, proliferation, metabolism, and pathogenesis of various diseases. O-GlcNAcylation is regulated by two enzymes, O-GlcNAc transferase (OGT), which adds a …
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Dissecting the Role of Novel O-GlcNAcylation of NF-κB in Pancreatic Cancer
… WT-p65. We also show that inhibition of NF-κB O-GlcNAcylation may mitigate gemcitabine resistance and enhance its efficacy in PDAC cells. Collectively, our study uncovers a novel aspect of NF-κB regulation, which could aid future therapeutic development by targeting O-GlcNAc transferase (OGT) in …
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The Effects of a 5-Day High-Fat Diet on Skeletal Muscle O-GlcNAcylation
… of a 5-day high-fat diet on skeletal muscle O-GlcNAcylation. In the proposed study, 13 non-obese, sedentary, college-aged males consumed a controlled diet for two weeks followed by a high-fat diet composed of 55% fat, 30% carbohydrate, and 15% protein. Muscle biopsies were taken from the vastus …
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O-linked N-acetylglucosamine in differentiation and gene expression of mouse and human pluripotent stem cells
… to serine or threonine residues (O-GlcNAcylation) of proteins has only recently been characterized and its overall role in cell signalling remains elusive to date. Recent studies suggest an essential role of O-GlcNAcylation on the viability and pluripotency of mouse and human …
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Role of O-GlcNAc in the Vertebrate Secretory Pathway
… approaches to demonstrate that site-specific O-GlcNAcylation of COPII proteins mediates their protein-protein interactions and modulates cargo secretion. We demonstrate that individual O-GlcNAcylation sites of SEC23A are required for its function in human cells and vertebrate development, …
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Signaling pathways regulating skeletal muscle metabolism and growth
… The dynamic post-translational modification O-GlcNAcylation has been shown to serve as a cellular nutrient sensor in a wide range of cells and tissues, yet its role in skeletal muscle and SCs remains unexplored. Here, we ablated skeletal muscle O-GlcNAc transferase (OGT), and thus …
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Investigating the role of UDP-<i>N</i>-acetylhexosamine pyrophosphorylases in regulating <i>O</i>-GlcNAcylation in human disease
… proteins are modified by O-GlcNAc (O-GlcNAcylation). Interestingly, O-GlcNAc homeostasis is regulated by a single pair of enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). O-GlcNAcylation has been found to modulate enzyme catalytic activity, protein stability, protein–protein …
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… 1000) with -N-acetylglucosamine (O-GlcNAc / O-GlcNAcylation) is a highly dynamic and essential process in metazoa. The attachment and removal of the sugar residue is regulated by two antagonistic enzymes. The O-GlcNAc transferase (OGT), which adds the sugar onto protein substrates and the …
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Computational structure analysis and prediction of Ser/Thr modified by O-GlcNAc in human proteins
… modifications of proteins by O-GlcNAcylation with a computational biology approach. The O-GlcNAc transferase (OGT), the enzyme that catalyses the protein O-GlcNAcylation, targets specific Serines and Threonines (S/T) of intracellular proteins. However, while other …
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Functional Analysis of the F-box protein Fbxl17
… binding partners Uap1, an enzyme involved in O-GlcNAcylation of proteins was identified most frequently. We demonstrate that Fbxl17 binds to UAP1 directly and inhibits its phosphorylation, which we propose regulates UAP1 activity. Knockdown of Fbxl17 expression elevated O-GlcNAcylation in breast …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… post-translational modification (O-GlcNAcylation) is the dynamic and reversible attachment of N-acetylglucosamine to serine and threonine residues of target proteins. It is abundant in metazoa, involving hundreds of proteins linked to a plethora of biological functions with …
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Metabolic support in left ventricular hypertrophy
… a significant increase in the level of protein O-GlcNAcylation. Conclusions: In this trial, treatment with GIK was associated with a significant improvement in post-operative haemodynamics. Patients with LVH may benefit from metabolic therapies at the time of surgery
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Regulating the stabilities of NOD1 and NOD2, two innate immune receptors, with sugars and chaperones
… to monitor the stability changes caused by O-GlcNAcylation, and it was found that wild type NOD1 and NOD2 were stabilized when modified by OGT. Lastly, NOD1 and NOD2 each form a complex with OGT and the 70 kDa heat shock protein (HSP70), which both function to stabilize these NLRs. …
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Identification of tumour-associated gycoproteins recognised by the lectin from Helix pomatia in breast cancer
… HnRNPs, Hsp 27 and ENO1 by HPA correlated with O-GlcNAcylation of these proteins. Interestingly, these HPA-binding glycoproteins were either absent or showed decreased levels in the non-metastatic breast cancer cell line BT474 and in ‘normal’ HMT3522. A comprehensive analysis of the breast cells …
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Understanding tetratricopeptide repeats of <i>O</i>-GlcNAc transferase
… as a potential reductionist model for the O-GlcNAcylation system where OGT-substrate interactions can be studied. Structural characterisation of T. terrenum OGT suggested that this enzyme is a catalytically competent OGT orthologue, while treatment of T. terrenum with OGT inhibitors …
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The regulation of TGFβ/BMP signalling by deubiquitylating enzymes
… and the regulation of TGFß/BMP signalling by O-GlcNAcylation has not been investigated. Endogenous SMADs1-3 bound OGT and pulled down potential O-GlcNAc modified proteins. Furthermore, SMAD4 was possibly O-GlcNAcylated, which implies that O-GlcNAc modification could regulate TGFß/BMP signalling. …
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O-GlcNAc-Mediated Protein-Protein Interactions in Cell Signaling
… and cell biological functions of vimentin O-GlcNAcylation, and may have broad implications for our understanding of the regulation of IF proteins in general. </p><p>Despite the extensive number of OGT and OGA substrates identified, including IFs, the substrate specificity of both of these …