Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"O-GlcNAc"”.
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Developing O-GlcNAc transferase inhibitors - insights from substrate recognition
… proteins (> 1000) with -N-acetylglucosamine (O-GlcNAc / O-GlcNAcylation) is a highly dynamic and essential process in metazoa. The attachment and removal of the sugar residue is regulated by two antagonistic enzymes. The O-GlcNAc transferase (OGT), which adds the sugar onto protein substrates …
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Role of O-GlcNAc in the Vertebrate Secretory Pathway
<p>O-linked β-N-acetylglucosamine (O-GlcNAc) exerts myriad effects on protein localization, activation, inhibition, stability, conformational changes, or degradation. However, the biochemical effects of O-GlcNAc on the vast majority of substrates is unknown. Recently, we and others have shown that …
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O-GlcNAc-Mediated Protein-Protein Interactions in Cell Signaling
… by O-linked β-N-acetylglucosamine (O-GlcNAc) is an essential signaling mechanism that affects diverse processes such as cell cycle, metabolism, and death. Aberrant signaling has been implicated in numerous human diseases including cancer and neurodegeneration. However, major aspects of …
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Function and inhibition of the mitochondrial O-GlcNAc transferase isoform
… post-translational modification (O-GlcNAcylation) is the dynamic and reversible attachment of N-acetylglucosamine to serine and threonine residues of target proteins. It is abundant in metazoa, involving hundreds of proteins linked to a plethora of biological functions with …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification that is regulated by two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAc hydrolase (OGA). While it is elusive how O-GlcNAc alters protein function, altered O-GlcNAc levels are associated with human diseases. …
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Computational structure analysis and prediction of Ser/Thr modified by O-GlcNAc in human proteins
… modifications of proteins by O-GlcNAcylation with a computational biology approach. The O-GlcNAc transferase (OGT), the enzyme that catalyses the protein O-GlcNAcylation, targets specific Serines and Threonines (S/T) of intracellular proteins. However, while other …
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The Effects of a 5-Day High-Fat Diet on Skeletal Muscle O-GlcNAcylation
… O-linked B-D-N-acetylglucosamine, known as O-GlcNAc, has recently been identified as an important posttranslational modification that responds to nutrient sensing and cellular stress. Unlike other PTMs, O-GlcNAc has only two cycling enzymes. Because of its novelty, little research has been …
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Reductionist animal models to probe protein O-GlcNAcylation
Protein O-GlcNAcylation is a reversible type of glycosylation of serine and threonine residues of nucleocytoplasmic proteins occurring in all animals examined to date. Its installation on protein substrates is carried out by O-GlcNAc transferase (OGT), and its removal by O-GlcNAc hydrolase …
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Development of a novel liquid chromatography based tool to study post-translational modifications
… chosen to be the basis of this study was the O-GlcNAc modification. Using the C2C12 mouse myoblast cell line, it was determined that the optimal conditions for producing lysates containing increased yields of O-GlcNAc modified proteins was to treat differentiated C2C12 cells with 10nM insulin, …
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Understanding tetratricopeptide repeats of <i>O</i>-GlcNAc transferase
… with O-linked N-acetyl-β-D-glucosamine (O-GlcNAc) was serendipitously discovered in 1984. During the following decades it became clear that intracellular O-GlcNAc levels respond to various stimuli, suggesting that this modification is dynamic and may represent a novel cellular signalling …
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O-linked N-acetylglucosamine in differentiation and gene expression of mouse and human pluripotent stem cells
… to serine or threonine residues (O-GlcNAcylation) of proteins has only recently been characterized and its overall role in cell signalling remains elusive to date. Recent studies suggest an essential role of O-GlcNAcylation on the viability and pluripotency of mouse and human …
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Beta-o-glicosilación de péptidos que incorporan aminoácidos no naturales
… Finally, despite the importance of beta-O-GlcNAc glycosylation in fundamental biological processes, sparse structural information is known about beta-O-GlcNAc-Ser/Thr motifs. For this reason, we report the synthesis and conformational analysis of the simplest model glycopeptides derived …
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The regulation of TGFβ/BMP signalling by deubiquitylating enzymes
… proteomic approach identified O-GlcNAc transferase (OGT) as an interactor of SMAD2. SMADs have not been associated with O-GlcNAc modifications and the regulation of TGFß/BMP signalling by O-GlcNAcylation has not been investigated. Endogenous SMADs1-3 bound OGT and pulled down …
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Investigating the role of UDP-<i>N</i>-acetylhexosamine pyrophosphorylases in regulating <i>O</i>-GlcNAcylation in human disease
… O-linked N-acetyl-β-<sub>D</sub>glucosamine (O-GlcNAc) was first observed on the surface of lymphocytes in 1984. It was then found that over 1,000 nucleocytoplasmic proteins are modified by O-GlcNAc (O-GlcNAcylation). Interestingly, O-GlcNAc homeostasis is regulated by a single pair of enzymes: …
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Regulating the stabilities of NOD1 and NOD2, two innate immune receptors, with sugars and chaperones
… associated variants are post-translationally O-GlcNAcylated by the enzyme O-GlcNAc transferase (OGT). A cellular thermal shift assay (CETSA) typically used to detect protein-ligand binding was adapted in order to monitor the stability changes caused by O-GlcNAcylation, and it was found that wild …
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Development of a chemical biology approach for glycoprotein discovery in Caenorhabditis elegans reveals glycosylated isoforms of multiple proteins with mitochondrial function
… role. I also tested the hypothesis that O-GlcNAc glycosylated isoforms of mitochondrial proteins are glycosylated at a consensus motif that is different from that of another sub-group of O-GlcNAc glycoproteins. By performing a meta-analysis on previously published glycosylation sites, I …
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Dissecting the Role of Novel O-GlcNAcylation of NF-κB in Pancreatic Cancer
… novel finding that the p65 subunit of NF-κB is O-GlcNAcylated at serine 550 and 551 upon NF-κB activation. Importantly, the overexpression of either serine-to-alanine (S-A) single mutant (S550A or S551A) or double mutant (S550A/S551A) of p65 in PDAC cells impaired NF-κB nuclear translocation, p65 …
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