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Showing 1 to 20 of 541 for “"Nucleotides."”.
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Analysis of Nucleosides and Nucleotides in Milk and Infant Formula
Nucleotides have been routinely supplemented to infant formulas due to the important roles they play in metabolism and to replicate the higher concentrations typically found in human milk. A method utilising anion exchange solid-phase extraction clean-up and liquid chromatography was developed for …
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Molecule recognition of nucleic acids, nucleosides, nucleotides, and their derivatives
… containing DNA building units such as nucleotides, nucleosides, and nucleobases have recently aroused much interest because of their versatile applications. In chapter 5, we report the synthesis of calix[4]arenes ( 5.11-5.14 ) functionalized with a single nucleobase (thymine, adenine, …
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Extracellular Nucleotides Induce Transient Elevation of Cytosolic Calcium in Articular Chondrocytes
Mechanical stimulation provokes release of nucleotides that can regulate cell behavior by binding to P2Y receptors. Our objective was to test the effect of nucleotides on P2Y receptor activity in live articular chondrocytes by monitoring changes in the concentration of cytosolic free calcium. ATP …
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The utility of modified nucleotides for high-throughput nucleic acid analysis /
Synthetic base-modified nucleic acids are widely used in various fields. The aim of this dissertation was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for …
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Studies on the detection of nucleotides and oligonucleotides by mass spectrometry
… is to develop novel methods for the detection of nucleotides, oligonucleotides, and modified nucleotides such as DNA adducts by mass spectrometry. DNA adducts are important because they are formed during chemical carcinogenesis as well as during anti-cancer chemotherapy. However, DNA adducts are …
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Measurement of cyclic nucleotides in human sperm: experimental and clinical application
… to assess essential second messengers. Cyclic nucleotides (cAMP and cGMP) play a crucial role as signalling molecules for capacitation, motility, and acrosome reaction in mammalian sperm. Given their importance in motility and fertilising ability, defective nucleotide function could be a cause …
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Human colon cell migration and socialization induced by small RNA nucleotides
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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Advances in Fast Atom Bombardment Mass-Spectrometry (Glycosphingolipids, Nucleotides, Markers, Negative Ion)
… and a series of protected oligonucleotides of known structure were analyzed to ascertain the potential utility of fast atom bombardment mass spectrometry in the structural elucidation of novel compounds in these classes. Negative ion mass markers, necessary for accurate mass …
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High pressure liquid chromatography of nucleotides and nucleic acid bases from fungi
… A. niger, F. moniliforme, and C. herbarum). Nucleotides were extracted from the mycelial mat in high yield with 10% (weight/volume) trichloroacetic acid, and then preseparated from interfering polysaccharides, glycoproteins, etc., on a Biegel P-2 column with double distilled water elution. …
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Novel fluorescent nucleotides for metabolic labelling and for the construction of DNA probes
Cílem této dizertační práce byla syntéza nových nukleosidů, nukleotidů a příslušných DNA sond nesoucích různé fluorescenční značky pro bioanalytické aplikace. V první části práce byl pomocí Sonogashiry cross-kapling reakce syntetizován 2'-deoxycytidin a jeho trifosfát nesoucí imidazolinový …
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The role of nucleotides and the P2X7 receptor in radiation induced bystander effect
An interesting phenomenon, with possible implications for treatment related to radiotherapy is known as the bystander effect, whereby cells that have been irradiated initiate the destruction of neighbouring cells that have not been irradiated directly. The exact mechanisms that result in the …
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Novel approaches to the synthesis of selenium modified nucleosides, nucleotides and nucleic acids
… phosphoroselenolate triesters, pure oligodeoxynucleotides were isolated using standard deprotection and purification procedures and subsequently characterised by mass spectrometry and circular dichroism. The CD spectra of both modified and native duplexes derived from self-complementary …
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The metabolism of adenosine nucleotides in thoracic muscle mitochondria from the American cockroach
Myokinase and three inorganic pyrophosphatases with pH optima of 6.4, 7.2 and 8.4 have been isolated and purified from the thoracic muscle mitochondria of the American cockroach. Myokinase was purified over 100-fold by heat and acid treatment, ammonium sulfate fractionation and column …
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Testing the Role of Cyclic Nucleotides in Regulating Ion Channels Essential to Pollen Fertility
It is unknown if plant cyclic nucleotide gated channels (CNGCs) are activated by cyclic nucleotide monophosphates (cNMPs, e.g. cAMP or cGMP), or even if cNMPs are present at physiologically significant levels in plants. cNMP presence in growing A.thaliana pollen tubes was visualized using …
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Synthesis of Non-Natural Cyclic Di-Nucleotides for the Investigation of Bacterial Signaling Pathways
… This family of molecules, the cyclic di-nucleotides, which includes c-di-GMP and c-di-AMP, are critical to regulating bacterial processes such as motility, glucose remediation, and cell wall homeostasis. Their importance has spurred numerous investigations into their mechanism of action. …
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Influx and Efflux of Adenine Nucleotides From Erythrocytes Regulate Oxygen Transport and Systemic Metabolism
<p>Erythrocytes are responsible for ensuring a sufficient supply of oxygen to meet activity demands. The mammalian erythrocytes primarily modulate their oxygen-carrying capacity by varying the levels of organic phosphates, such as 2,3-BPG. In other organisms, such as fish, ATP instead of 2,3-BPG is …
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A Kinetic Investigation of the Mechanism of Muscle Contraction with a Series of Nucleotides
<p>Muscle contraction is thought to be accomplished by sliding of myosin filaments along actin filaments. Although actomyosin naturally uses ATP as an energy source, it can also use other nucleoside triphosphates (NTP) as substrates. In this work, the dependence of rate and equilibrium constants of …
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