Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Nucleotide analogue"”.
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Developing a Novel Cell Surface RNA Detecting and Profiling Method via RNA Metabolic Labeling
… csRNAs are 4-Thiouridine (4sU), an unnatural nucleotide analogue, and S-(2-aminoethyl)-ester-methanesulfonothioic-acid-biotin (MTSEA-biotin), a crosslinker designed specifically to label 4sU. By deploying these tools to cell lines such as HEK293T and HeLa, csRNA is detectable by Enhanced …
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Functional Identification of MicroRNAs Counteracting Cellular Senescence
… were the analysis of the incorporation of the nucleotide analogue EdU, as a measure of cell proliferation, and the level of the cell cycle kinase inhibitor p21 as an indicator of G1 cell cycle arrest. Twenty miRNAs were identified that exerted a strong replicative effect on senescent cells (up …
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Notch activation and clone fate in intestinal homeostasis and inflammation
… dynamics in the colonic epithelium. Using double nucleotide labelling and pulse-chase lineage tracing experiments along with immunofluorescence and immunohistochemistry methods with a transgenic Notch active mutant mouse model (N1ICD<sup>ΔIEC</sup>), cell and clonal dynamics were determined in the …
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Exploring the role of endogenous DNA damage in neurodegeneration
… its specific synthetic lethal relationship with nucleotide analogue drugs. In addition, I performed a whole genome CRISPR/Cas9 screen. Subject to validation, the screen demonstrated that inhibition of proteins in key pathways, such as chromatin regulation and transcription, rescued the synthetic …
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Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation
… of messenger RNA. These experiments employed a nucleotide analogue, 5'-guanylyl-β,γ-methylenediphosphonate, which was shown to be a non-competitive inhibitor of protein synthesis. The presence of this inhibitor increases the time required to hydrolyze a guanosine-5'-triphosphate molecule. Since …
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Analyses of RNA dynamics in Mus musculus
… RNA. By exposing the mouse embryos to a nucleotide analogue in a stage-specific manner, I identified genes that are actively transcribed in the 2-cell embryos. This method would be useful in studying the transcriptional cascade in the early mammalian preimplantation embryos.
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INVESTIGATION OF ANTIBIOFILM AND ANTIVIRULENCE ACTIVITY OF 5-FLUOROCYTOSINE IN ESCHERICHIA COLI
… antimetabolites and nucleobase/nucleoside analogues, such as 5-fluorocytosine and 5-fluorouracil, have been shown to possess antivirulence activity in Gram negative bacteria like Pseudomonas aeruginosa and Escherichia coli. In my PhD work, based on previous observation that pyrimidine …