Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Nucleoporin"”.
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Biochemical and Structural Analysis of the Nucleoporin Nup214 and its Involvement in mRNA Export
<p>In order to gain a deeper understanding of the role of nups in leukemogenesis, and to make sense of the architecture and regulation of the mRNA export machinery at the NPC, I set out to biochemically and structurally characterize Nup214. In this thesis, I present the crystal structure of the …
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Quantitative super-resolution imaging of cell polarity proteins using DNA-PAINT
… efficiency of target proteins. Moreover, I used nucleoporin proteins in the nuclear pore complex to calibrate the influx rate of fluorescently-labelled DNA to quantify the number of molecules in super-resolution images. Additionally, I used nucleoporin-160 and nucleoporin-188 to benchmark …
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Expression Profiling of Transgenic Arabidopsis Over- and Underexpressing SR45, a Plant-Specific Serine /Arginine -Rich Factor
… a number of important transport factors (nucleoporin 155, exportin 1) as well as several stress-related transcripts that may represent secondary downstream targets. As a result of these secondary effects, it is likely that SR45-silenced plants are more susceptible to stress.
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Understanding biological hydrogel function through design of simplified peptides and polymers
… as the phenylalanine-glycine (FG) repeats in nucleoporin proteins of nuclear pore complexes (NPCs) and the proline-threonine-serine (PTS) domains in mucin polymers found in mucus.
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Tap /Nxf1 N -Terminal Domain Mediates Homotypic Complex Assembly and Is Necessary for Nuclear RNA Export
… protein that interacts directly with nucleoporin proteins of Nuclear Pore Complexes (NPCs) as well as messenger RNA cargo. It has been long understood that the NXF family is unique from the Karyopherin transport receptor family and that RNA export functions independently of the nuclear …
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Characterisation of ALADIN’s function during cell division
… of the nuclear pore complex (NPC) also called nucleoporins. NPCs are large complexes studded in the nuclear envelope, which control the nucleocytoplasmic transport. It is now known that nucleoporins participate in spindle assembly, kinetochore organisation, spindle assembly checkpoint, and all …
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Architecture of a Coat for the Nuclear Pore Membrane
… protein components of NPCs, termed collectively nucleoporin, have been identified, how nucleoporins arrange in NPC, however, is still an enigma. NPC is a highly symmetric protein complex, which contains an eight-fold rotational symmetry across the center of the pore and a two-fold symmetry in the …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
… technology to a heavily O-GlcNAc-modified nucleoporin NUP98 and NUP98 leukemogenic fusions, produced under chromosomal translocation, to gain insight into the mechanism of NUP98 fusion-mediated cell transformation in leukemia. The wild-type nucleoporins are associated with nuclear …
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Die Funktion von NLP1 im CRM1-abhängigen Protein-Export aus dem Zellkern
… Arbeit war die Analyse des Proteins NLP1 (nucleoporin like protein 1). Da andere Gruppen mittels Hefe-zwei-Hybrid-Analysen eine Interaktion zwischen CRM1 und NLP1 nachgewiesen hatten, sollte nun die Funktion von NLP1 im CRM1-abhängigen Proteinexport näher untersucht werden. Es konnte in …
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Structural studies of large architectural nucleoporins and coat proteins
… envelope. It assembles from ~30 proteins, termed nucleoporins or nups, symmetrically arranged about a central 8-fold axis. Some nucleoporins also contribute to other structures or perform diverse other functions. A subset forms a stably-associated core scaffold for the NPC, organized into two …
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Discovery and characterization of prions in Saccharomyces cerevisiae
… another lab, establishes that Nup100, a GLFG nucleoporin, is a prion. The conformational flexibility of GLFG nucleoporins is critical for the function of the nuclear pore complex, a molecular sieve that regulates all macromolecular transport between the nucleus and cytoplasm.
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A link between c. Elegans morphogenesis and mrna export
… (co-IP) the NXF-1 binding partner NXT-1/p15, nucleoporin NPP-9/RanBP2 and P granule protein GLH-1. We suggest that NPP-9/RanBP2 probably mediates the translocation step across nuclear pore complex (NPC) and that GLH-1 probably mediates the release into the cytoplasm. Furthermore, co-IP of …
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Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
… assembly between the Nspl-complex and a fourth nucleoporin (Nup) Nic96. Nic96 harbors a 20 kDa coiled-coil domain at the N-terminus followed by a 65 kDa stacked helical domain. The coiled-coil domain of the Nspl -complex and the N-terminus of Nic96 combine to form a tetrameric assembly, …
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Structural and biochemical analysis of the Y-shaped Nup84 subcomplex of the NPC
… Overall, the NPC is composed of -30 proteins, or nucleoporins (nups), that form biochemically stable subcomplexes which are repeated in multiple copies to assemble the intact NPC. This macromolecular machine is not only essential to nucleo-cytoplasmic transport, but also plays a pivotal role in a …
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Innate immune modulation and pathogenic basis for porcine reproductive and respiratory syndrome virus
… Nsp1β was found to specifically interact with nucleoporin 62 (Nup62). Nup62 is one of the major components of the nuclear pore complex (NPC) that form channels spanning the double lipid bilayer of the nuclear envelope for nucleocytoplasmic transport of cellular molecules including host mRNAs. A …
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Investigating nuclear transport proteins as secreted cancer biomarkers
… STRING bioinformatic analysis. These include nucleoporin 214 (Nup214), Prem RNA 3'-end-processing factor FIP1 (FIP1L1), cell division cycle and apoptosis regulator 1 (CCAR1), cleavage and polyadenylation specific factor 7 (CPSF7), ribosomal protein L7 (RPL7), ribosomal protein L10 (RPL10), …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
… a crucial role for the phenylalanine-glycine nucleoporins (FG-Nups). I constructed a coarse-grained model to investigate the dynamics of these proteins. My coarse-grained model was able to recapitulate in vivo experimental results previously measured in our lab. My results also suggest that, …