Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"Nucleolus"”.
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Proteomics and dynamics of the human nucleolus
The nucleolus is the most prominent structure within the eukaryotic cell nucleus and it was established to be the site where the majority of ribosomal RNAs (5.8S, 18S and 28S) are transcribed, processed and assembled with ribosomal proteins to form ribosomal subunits. The sole role of ribosome …
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Characterization of a novel family of nucleolus localized RNAs
… various non-membranous sub-nuclear domains with nucleolus being the most prominent one. The nucleolus is the largest sub-nuclear domain where ribosomal gene transcription, pre-rRNA processing, and the initial steps of ribosome sub-unit assembly take place. In human cells, nucleoli are formed …
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The functional characterization of a novel protein, Liat1
… in the dense fibrillar compartment (DFC) of the nucleolus. Mutational analysis revealed that a low complexity, poly-lysine region contained in the N-terminal intrinsically disordered region of Liat1 is required for its self-interaction and nucleolar phase separation. In addition to Ate1, Liat1 …
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rRNA transcription as potential therapeutic target in triple negative breast cancers
… abnormal cell growth. In eukaryotic cells, the nucleolus is the region of the nucleus where initial stages of ribosome production occur. These stages include synthesis of ribosomal RNAs by RNA Polymerase 1 (RNAP1) followed by its processing and modification. For decades, the size and number of …
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Nucleolar dynamics in cellular senescence
The nucleolus is a subnuclear structure widely known as the site of ribosome biogenesis. Recently, additional functions of the nucleolus have been described, one of which is serving as a stress sensor. When the nucleolus senses stressors, it causes nucleolar stress characterised by changes to …
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Investigations into Why Human (but not mouse) KU70/80 is Essential Reveal a Role for Human KU70/80 in Ribosome Biogenesis
… but not mouse Ku70/80 is enriched in the nucleolus at the Dense Fibrillar Component (DFC) periphery, likely via co-evolved protein-protein interactions with nucleolar factors. Consistent with its localization in the nucleolus, human Ku70/80 null cells exhibit ribosomal RNA (rRNA) …
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Diagnostische und prognostische Relevanz der AgNOR-Analyse bei Osteosarkomen
… AgNOR-Färbemethode werden argyrophile (Ag), den ?Nucleolus organisierenden Regionen? (NOR) assoziierte Proteine im Nucleolus selektiv dargestellt. In verschiedenen Studien wurde die Bedeutung der Quantität der AgNOR-Partikel bzw. deren Fläche (NORA) als Marker für zelluläre proliferative Aktivität …
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Importance of hierarchical interactions in concentrated protein systems
… provide functionality to the condensate. The nucleolus is a large membrane-less organelle in the nucleus which is responsible for the assembly of ribosomes. We have developed models to understand how interactions among major proteins assist pre-ribosomal assembly and proper rRNA folding in the …
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A Unified View of Protein Low-complexity Regions (LCRs) Across Species
… space to higher-order assemblies, such as the nucleolus, metazoan extracellular matrix and plant cell wall, we relate LCR functions across different fields and suggest that LCR functions may be unified in their roles in higher-order assemblies. Using this unified view, we uncover …
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Molecular visualization and localization of ribosomal subunits interaction in cells
… I also I 2 detected a strong signal in the nucleolus. These observations suggest that either there is translation in the nucleolus or that, contrary to what is currently understood, ribosome subunits can join together during ribosome biogenesis. In summary, with the technique I have …
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Genomic and microscopic dissection of nuclear compartmentalization and function
… to nuclear compartments (speckles, lamina, and nucleolus), and its dynamics and function. In Chapter 2, I first improved TSA-Seq with 10-20-fold higher sensitivity (TSA-Seq 2.0) to enable efficient mapping of genome organization relative to nuclear speckles. I applied TSA-Seq 2.0 to mapping …
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Discovering non-equilibrium mechanisms that regulatestructure and function of biomolecular condensates usingphase-field modeling
… project focuses on the fibrillar center of the nucleolus, an important condensate that is involved in ribosome biogenesis. Using a phase-field model that explicitly accounts for rRNA-protein interactions in the nucleolus and the non-equilibrium reaction of rRNA transcription, we show that the …
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Deterministic Barcoding of Neuron Identities through Multicolor Fluorescent Markers in C. elegans
… colors. We also tag subcellular markers of the nucleolus and nuclear membrane with fluorescent proteins to allow for a combinatorial barcode for neuron types, allowing the differentiation of neurons at a single-cell resolution. Through multicolor barcoding of individual neurons, we distinguish …
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Genetic and cytological investigation of Nucleostemin-1 in Drosophila melanogaster
… over-expressed GFP-NS1 localizes throughout the nucleolus and nucleoplasm, and to several transcriptionally active inter-bands of polytene chromosomes. Over-expression caused melanotic tumors and larval and pupal lethality. RNAi depletion of NS1 caused a loss of imaginal (precursor) cells in the …
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Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
… example of RNA-scaffolded structures is the nucleolus, a subnuclear body that serves as the site of ribosome biogenesis, cell cycle regulation, and stress responses. Nucleolar assembly is initiated de novo after each cell division—nucleated by pre-ribosomal RNA (pre-rRNA) and other nucleolar …
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eL15 and eL22 proteins and their functional environments during ribosome assembly in Saccharomyces cerevisiae
… regulated and organized that starts in the nucleolus and orderly progresses through the nucleoplasm; almost mature ribosomal particles are exported to the cytoplasm, where the last steps of maturation take place, previously to their involvement in the translation process. The role of the …
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Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells
… cells. Very little Py I or II* is found in the nucleolus or in the cytoplasm. These two forms of Py DNA are located in the same sub-nuclear fraction. At least half of each is not in association with the nuclear membrane. Possible locations for the rest of the Py DNA are discussed. A study of the …
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