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Showing 1 to 20 of 33 for “"Nucleoid"”.
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Nucleoid-Associated Proteins of Streptomyces coelicolor: Discovery and Functions
… problematic. The role of the architectural nucleoid-associated proteins (NAPs) in regulation of the expression of these products has been neglected within the Actinomycetes. NAPs are small, highly abundant proteins which govern both gene expression and nucleoid structure on a genome-wide …
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Nucleoid condensation in Escherichia coli by the DNAbinding protein SymE
… is likely due to its accumulation in the nucleoid and subsequent severe nucleoid condensation. This condensation is accompanied by DNA damage. Nucleoid compaction, DNA damage and global downshifts in transcription and translation are consistent with lethal levels of the E. coli nucleoid …
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Molecular Mechanism By Which The Escherichia Coli Nucleoid Occlusion Factor, Slma, Keeps Cytokinesis In Check
… by two key regulatory systems called the Min and nucleoid occlusion (NO) systems. In E. coli, Min proteins oscillate from pole to pole; the net result of this oscillatory process is the formation of a zone of FtsZ inhibition at the cell poles. However, the replicated nucleoid DNA near the midcell …
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Electrofusion of Escherichia coli with Giant Lipid Vesicles and the Compaction of Escherichia coli Nucleoid
The fusion of lipid membranes is a key step in genetic manipulation technologies, and a ubiquitous phenomenon observed in many aspects of cellular activities. To date, various methods have been developed for artificially inducing the fusion of biological and synthetic lipid membranes/vesicles to …
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Interactions between nucleoid associated proteins and DNA in the presence of mechanical and chemical forces
… interact with DNA in prokaryotes are known as nucleoid associated proteins (NAPs). These proteins play a vital role in shaping the DNA and assist in many cell processes, including gene expression, replication, and transcription. NAPs have been studied extensively in order to elucidate how their …
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Establishing how bacterial cells position the division site
… action of two factors: the Min system and nucleoid occlusion. Both factors have been proposed to prevent Z ring assembly along the length of the cell, allowing it to assemble only once chromosomes segregate and nucleoid occlusion is relieved specifically at midcell. The research described …
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Analysis of the Nucleioprotein Complexes Essential for P1 Plasmid Partition
… a novel ParA interaction with the host nucleoid that is critical for proper P1 plasmid dynamics in vivo. Finally, I demonstrate how ParA can act as an adaptor between the nucleoid and the partition complex; effectively allowing the plasmid to use the nucleoid as a track for its …
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Super-Resolved Microscopy as a Tool to Unveil Intracellular Structure in Biological Systems
… the spatial redistribution of the heat-stable nucleoid structuring (H-NS) protein within E. coli in response to high osmolarity. H-NS is an abundant nucleoid association protein in gram-negative bacteria and plays a dual role as a global gene regulator and nucleoid organizer. By imaging the …
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New mechanisms in transcription regulation and chromosome organization in Caulobacter crescentus
… and organized inside cells. In bacteria, nucleoid-associated proteins (NAPs) play critical roles in shaping and compacting the chromosome by bending, wrapping and bridging the DNA. The binding of these proteins can also profoundly affect gene expression regulation. In this work, I have …
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Rate zonal density gradient ultracentrifugation analysis of repair of radiation damage to the folded chromosome of Escherichia coli
The structure of the membrane-free nucleoid of Escherichia coli and of unfolded chromosomal DNA was investigated by sedimentation on neutral sucrose gradients after irradiation with 60Co gamma-rays and ultraviolet light (2S4nm). Irradiation both in vivo and in vitro was used as a molecular probe of …
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The iron-dependent cyanide and hydrogen peroxide co-toxicity in Escherichia coli and its catastrophic consequences for the chromosome
… linear DNA degradation and disintegration of the nucleoid structure. We have shown that this disassembly of the nucleoid structure following the removal of CN and H2O2 is affected by the nucleoid-associated proteins and depends on ongoing transcription, thereby revealing the role of transcription …
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Studies of the excretion of aluminium by the kidney and the toxic effects of the element on DNA
… was assessed by the increase in size of cell nucleoids (ie DNA in supercoiled form). Nucleoid size was analyzed in a Epics 753 Fluorescence Activated Cell Sorter interfaced with an MDADSII data acquisition and analysis system. After 90 min incubation with Al (over the concentration range …
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DNA -Binding Proteins That Facilitate Integration of Transmissible DNA Elements in Bacteroides Thetaiotaomicron
… protein that is most likely involved in nucleoid organization, BHP2, a homolog to BHP that probably functions in a similar capacity, and BT 1847, a specific DNA-binding protein that is a member of the AraC/Xy1S transcription regulator family. Preliminary data suggest that another protein, …
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APPROACHES TO IDENTIFY SURFACE PROTEINS OF ANAPLASMA PHAGOCYTOPHILUM DENSE-CORED ORGANISMS AS ADHESINS TO HUMAN P-SELECTIN GLYCOPROTEIN LIGAND-1
… dense-cored cell (DC), which has a dense nucleoid, and a larger, pleomorphic electron lucent reticulate cell (RC), which has a dispersed nucleoid. We determined that the respective roles of the A. phagocytophilum DCs and RCs are adherence/infection and vacuolar replication, respectively, …
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Transposable prophage Mu exists as an independent chromosomal domain in E. coli
… the Mu L end, the MuB protein, and the E. coli nucleoid-associated proteins IHF, Fis and HU. The Mu domain was observed at two structurally different chromosomal locations, and was specific to the Mu prophage, i.e. was not observed for the [mathematical symbol] prophage. A model is proposed that …
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The regulation of genes of unknown function implicated in nitrosative stress tolerance in Escherichia coli K-12
… and two sites in the \(ogt\) promoter. The nucleoid-associated protein, Fis, repressed transcription from both promoters, especially in rich medium, by binding to sites that overlap the NarL site, excluding the essential activator. Both promoters were more active in the absence of functional …
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Role of protein kinase C isozymes in cellular senescence and reversal of senescence in response to 12-O-tetradecanoylphorbol-13 acetate (TPA) treatment in
… (BrdU) incorporation at the mitochondrial nucleoid along with mitochondrial transcription factors A (TFAM). Nevertheless, we observed that mitochondria dysfunction was increased via alteration of ROS level, integrity of membrane potential, mitochondria cristae structure and ATP content. To …
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Molecular mechanisms underlying regulation of type III secretion system upstream of the master regulator HrpL in Erwinia amylovora
… hrpL and other T3SS gene expression. IHFs are nucleoid-associated proteins and consist of two subunits, i. e. IHFα and IHFβ. IHFα and IHFβ usually form heterodimers, which could influence nucleoid structure and gene expression via DNA bending. Two single mutants (ihfA and ihfB) were generated …
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Characterization of FMP35: A novel gene and its role in mitochondrial DNA stability
… an essential DNA-protein complex called a nucleoid. One protein that has been defined as a mitochondrial protein is Fmp35p. This is a novel protein that remains uncharacterized. A fmp35?::URA3 gene knockout yields a p- phenotype as illustrated by a respiration loss assay. Furthermore, a …
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H-NS Counter-silencing as a Primary Regulatory Mechanism of Curli Gene Expression in Salmonella Typhimurium
… Salmonella enterica serovar Typhimurium. The nucleoid-associated protein H-NS is able to recognize and bind AT-rich horizontally acquired genes, such as the csg regulon required for curli synthesis, and silence their expression. While previous studies have implicated a role of the alternative …
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