Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 22 for “"Nucleocytoplasmic transport"”.
-
The function of Nup358 in nucleocytoplasmic transport
Nukleocytoplasmatischer Transport erfolgt durch in der Kernmembran verankerte Porenkomplexe. Von einigen Nukleoporinen, den Komponenten der Kernporen, wird angenommen, dass sie eine Rolle als Andockstation für Transportkomplexe auf ihrem Weg in bzw. aus dem Kern spielen können. Das Nukleoporin …
-
Molecular Size and Charge Effects on Nucleocytoplasmic Transport Studied By Single-Molecule Microscopy
… as highly-selective machinery for bidirectional nucleocytoplasmic transport. The selective barrier, which is formed by the natively unfolded phenylalanine-glycine (FG)-nucleoporins (Nups), in the NPC allows for two transport modes: passive diffusion of small signal-independent molecules (< 20-40 …
-
Biochemical Analysis of the Protein-Protein Interactions Involved in Karyopherin-Mediated Transport Across the Nuclear Pore Complex
<p>Nucleocytoplasmic transport occurs through the nuclear pore complex (NPC), which in yeast is a highly symmetric ~50 MDa complex consisting of approximately 30 different proteins. Small molecules can freely exchange through the NPC, but macromolecules larger than ~40 kDa such as proteins, mRNAs, …
-
Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
… a large, modular protein assembly that regulates nucleocytoplasmic transport in all eukaryotes. The ~60-120 MDa NPC is a modular assembly of multiple copies of ~30 distinct proteins that are arranged into biochemically distinct sub-complexes. We believe that the structural characterization of the …
-
Characterization of Nuclear Localization Signals of Karyopherin-Mediated Nuclear Import
Nucleocytoplasmic transport is mediated by Karyopherin beta (Kap beta) proteins in a Ran-dependent manner. Ten import Kap betas recognize their cargos through the nuclear localization signals (NLSs) and carry them into the nucleus. Recent structural and biochemical work on Kap beta2 (or …
-
Transcriptomics-based study reveals a novel link between arginine methylation and phosphate regulation in Saccharomyces cerevisiae
… as transcription, transcriptional regulation, nucleocytoplasmic transport of proteins and mRNAs and RNA splicing. Hmt1 is also known to mediate protein-protein and protein-RNA interactions. Despite its well-studied role in regulatory processes, the effects of Hmt1 knockout on the transcriptome …
-
Characterisation of ALADIN’s function during cell division
… in the nuclear envelope, which control the nucleocytoplasmic transport. It is now known that nucleoporins participate in spindle assembly, kinetochore organisation, spindle assembly checkpoint, and all processes important for genome integrity maintenance. This work demonstrates that the …
-
Structural elucidation of a common architecture of the nuclear pore complex and COPIl vesicle coats
… envelope and form the exclusive passageway for nucleocytoplasmic transport. NPCs play critical roles in molecular transport and a myriad of other cellular processes. Elucidation of the structure of the NPC is thus expected to provide important insight into cell biology. In this thesis, I …
-
Proteomic, Bioinformatic and Functional Characterization of the Nuclear Pore Complex of the African Trypanosome
… membrane, known as the nuclear envelope (NE). Transport across the NE is mediated by large protein assemblages known as nuclear pore complexes (NPCs). Yeast and vertebrate NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are present in multiple copies. The origins and …
-
A Proteomic Approach to Elucidating the Function of Picornavirus 2A Protease
… metabolism, protein synthesis, nuclear and RNA transport across the nuclear pore complex, apoptosis, and innate immune responses. Picornaviruses are the most dominant human disease-causing viruses and present an excellent clinical target for research studies into their molecular mechanisms. …
-
Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
… is a dynamic macromolecular machine. Protein transport is regulated by the small G protein Ran. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nupl53 at the nuclear face and Nup358 at the cytoplasmic face. Both Nups contain distinct RANBP2 type zinc finger …
-
Toxic PRn Poly-Dipeptides Encoded by the C9orf72 Repeat Expansion Block Nuclear Import and Export
… cells were treated with PRn. The functional nucleocytoplasmic transport defect caused by PRn peptide was due to binding of the peptide to the central channel of the NPC as visualized by super-resolution microscopy. The NPC central channel is comprised of phenylalanine-glycine (FG) domain …
-
Structure and Organization of Nuclear Pore Proteins
… be crucial to understanding the mechanism of nucleocytoplasmic transport, but due to its size, the NPC presents a challenge to structure determination. There are electron microscopy (EM) structures of the entire NPC, and high resolution crystal structures of some of the individual components …
-
Identification and characterization of Risp
… of viral structural proteins. Rev enhances the nucleocytoplasmic transport and the translation of unspliced and single spliced viral mRNAs by binding with high affinity to a specific target element on the HIV-RNA. It was assumed that interaction with cellular factors is essential for Rev …
-
Innate immune modulation and pathogenic basis for porcine reproductive and respiratory syndrome virus
… double lipid bilayer of the nuclear envelope for nucleocytoplasmic transport of cellular molecules including host mRNAs. A region representing the C-terminal 328-522 residues of Nup62 was identified as the binding domain to nsp1β. Mutational studies revealed that leucine 126 of nsp1β was the …
-
Impact of mechanical signals on human foetal lung cell differentiation
A major function of the lung is as a mechanical pump to bring oxygen-rich air into the body, allow gas exchange to occur, and remove carbon dioxide-rich air. It is increasingly recognized that physical forces, such as tension, compression, or shear, as well as passive mechanical material properties …
-
The Effect of Interleukin-1 (IL-1) Concentration on Single Cell NF-kappaB Activation in a Gradient-Generating Microfluidic Device
… transcription and gene expression through nucleocytoplasmic transport. Most previous studies on NF-κB activation have been performed using bulk assays to look at populations of cells. Determining cell variance at a single-cell level is crucial in understanding the full mechanisms of drug …
-
Investigating Mechanisms of Nuclear mRNA Export
In eukaryotes, the segregation of the RNA synthesis/processing in the nucleus from the translation machinery in the cytoplasm has important implications for nuclear export and retention of mRNAs. This division ensures that only correctly processed mRNAs are exported to the cytoplasm to be …
-
Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa (Baker) and its response to dehydration stress
… The third group contained proteins involved in nucleocytoplasmic transport (10%). This might reflect the capacity of this plant to control the movement of molecules to and from the nucleus during dehydration and the importance of this process in adaptation to dehydration stress. The fourth group …
-
SUMOylierung von NFYA
… Dies legt eine Verbindung zwischen Kerntransport und SUMOylierung nahe. Um dieser Vermutung nachzugehen, mussten zunächst RanBP2-abhängige SUMO-Substrate identifiziert werden, da zu Beginn der Arbeit noch keine physiologischen RanBP2 Substrate bekannt waren. Dies gelang mit dem Nachweis, …
Page 1 of 2