Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Nucleocytoplasmic shuttling"”.
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The functional motifs for nucleocytoplasmic shuttling of C-terminal fragment of c-Met : A novel "pH-dependent' nuclear localization signal
… (NES) in c-Met which plays a decisive role for nucleocytoplasmic shuttling of the receptor. Taken together, these results suggest that the shuttling of the C-terminal fragment of c-Met between the cytosol and nucleus appears to be due to the presence of both a putative NLS and an NES in the …
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Investigation of the Function and Regulatory Mechanisms of the Mammalian Target of Rapamycin
… between the nucleus and the cytoplasm, and this shuttling is required for its cytoplasmic signaling. I have further investigated the regulation and function of mTOR cytoplasmic-nuclear shuttling. A systematic screen of mTOR revealed a rev-like NES sequence within mTOR at amino acids 1281--1289, …
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Signaling mechanisms that suppress the anabolic response of osteoblasts and osteocytes to fluid shear stress
… contain an adhesion-associated protein and a nucleocytoplasmic shuttling transcription factor. “GO” mechanosomes functions to promote the anabolic response of bone to mechanical loading, while “STOP” mechanosomes function to suppress the anabolic response of bone to mechanical loading. While …
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Characterizing the Role of Gds1p in the Saccharomyces cerevisiae Environmental Stress Response
… dependent on a regulatory network centering on nucleocytoplasmic shuttling but also includes a range of other mechanisms. The acetyltransferase complex, NuA4 has been implicated in repression of Msn2p yet the mechanism is largely unknown. Gds1p is an uncharacterized yeast protein identified in a …
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Molecular Mechanism of Insulin Resistance: Role of mTOR Signaling Pathways
… of the protein---potentially regulated by a nucleocytoplasmic shuttling signal. The loss of nuclear import was accompanied by the significantly reduced ability of the L545A/L547A mutant to activate S6K1 in cells. Most importantly, when nuclear import was restored in the L545A/L547A mutant by …
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Understanding the adhesome network in primary and metastatic cutaneous squamous cell carcinoma
… function in metastatic SCC cells and that the nucleocytoplasmic shuttling of Mena is regulated by integrin activation and focal adhesion kinase (FAK). Moreover, our preliminary results suggest that Mena may act as a molecular clutch for the mechanosensing function of Nesprin-2 with actin. The …
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Characterization of zebrafish zipper-interacting protein kinase
… in vitro. Human and zebrafish ZIPK underwent nucleocytoplasmic shuttling without PAR-4 interaction, unlike rodent ZIPK, which required PAR-4 for nuclear exit. Unlike human ZIPK, zebrafish ZIPK TT299/300AA mutants were able to undergo shuttling. Similar to human ZIPK, catalytic mutations to …
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Novel Roles of PACS-1 Within the Nucleus and the Regulated Secretory Pathway
… a novel interaction between PACS-1 and the nucleocytoplasmic RNA-binding protein polypyrimidine tract-binding protein 1 (PTBP1), unveiling a PACS-1 nuclear trafficking function. Second, we defined a role for PACS-1 orchestrating intracellular calcium levels within corticotropic cells. We …
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p53 nuclear localization control, and p53-dependent regulation of DNA repair gene transcripts
… that the p53 negative regulator HDM2 was a nucleocytoplasmic shuttling protein that binds and carries p53 from the nucleus of the cell to the cytoplasm where it is destroyed by the proteasome. We determined that HDM2 with a mutated nuclear export sequence was still able to able to alter …
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Mechanisms of Human LARP6 Nuclear Export
… Previous work suggested that LARP6 undergoes nucleocytoplasmic shuttling, supported by the identification of a putative nuclear export sequence (NES) and to localization in both the nucleus and the cytoplasm. Subsequent high-resolution structures of the RNA binding domain of LARP6 showed that …
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Subcellular Localization of Human T-cell Leukemia Virus Type 1 Tax Oncoprotein
… respectively), but the mechanisms regulating nucleocytoplasmic shuttling and targeting of Tax to distinct subcellular regions have yet to be determined. In this study we identified regions in Tax that regulate nucleocytoplasmic shuttling and dictate subnuclear targeting.</p> <p>We identified …
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Investigating the cellular localisation of DEAD box helicase DDX3
… DDX3 to cytoplasmic speckles, and prevented the nucleocytoplasmic shuttling of DDX3. We found DDX3 to be recruited to Stress Granules after cellular stress. DDX3's cellular localisation and expression levels were also found to change throughout the cell cycle.
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Characterization of the Role of Glutaredoxin 3 and Its Binding Partners In Maintaining Iron Homeostasis In Saccharomyces Cerevisiae
… regulated by the transcription factor Aft1. Nucleocytoplasmic shuttling of Aft1 is dependent upon mitochondrial Fe-S cluster biosynthesis via a signaling pathway that includes the cytosolic monothiol glutaredoxins, Grx3 and Grx4. The crystal structure of the thioredoxin-like domain of Grx3 …
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Determining Mechanisms by which Pathological Extracellular Matrix induces Ocular Hypertensive Phenotypes in Human Trabecular Meshwork Cells
… (p<0.001) stiffer, associated with impaired nucleocytoplasmic shuttling of inactive β-catenin, reduced Cadherin-11, and key Wnt target genes/proteins; and increased cytoplasmic TAZ levels, reduced YAP levels in the nucleus and critical YAP/TAZ target genes/proteins. Wnt activation rescued hTM …
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Analysis of SMAUG1 protein motifs and interacting partners - linking regulation of phase separation to human disease
… assays, we showed that SMAUG1 undergoes nucleocytoplasmic shuttling and identified a functional C-terminal nuclear export signal that promotes rapid export to the cytoplasm via exportin-1. Crucially, investigation of SMAUG1’s RNA targets in and out of condensates revealed a potential …
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Regulation of SRC-3 localization and dynamics by phosphorylation during ERα-dependent transcriptional activation
… (IKKs) (41,42,43). Here, we report SRC-3 to be a nucleocytoplasmic shuttling protein, whose cellular localization is regulated by phosphorylation and interaction with ERalpha. Using a combination of high throughput and fluorescence microscopy, we show that both chemical inhibition (with U0126) and …