Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Nucleocapsid protein"”.
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Mutational Analysis of HIV-1 Nucleocapsid Protein and Methods Development for Aptamer Discovery
… studies related to the interaction between proteins and nucleic acids and their applications. The work reported here includes two projects. The first part of the thesis is on the mutational and combinatorial analysis of HIV-1 nucleocapsid protein (NCp7). Sixteen mutants of NCp7 were …
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The Interaction Between Host G3BP and Viral Nucleocapsid Protein Regulates SARS-CoV-2 Replication
<p>G3BP1/2 are RNA-binding proteins that promote condensation to form stress granules in response to various cellular stresses, including viral infection. G3BP1/2 are prominent interactors of the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the …
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The Expression of Shuni Virus Nucleocapsid Protein in Nicotiana benthamiana for Use as a Diagnostic Reagent
… of diagnostic reagents centred around the nucleocapsid (N) protein of the SHUV. The N proteins of closely related members of the order Bunyavirales have shown to be highly abundant in infection and cause an immune response in the infected hosts thus making it the ideal target. Using …
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The Efficacy of DNA Vaccine Using a Sindbis Virus-based Vector Against Nucleocapsid Protein of Hantaan Virus
… and humoral immune responses against the HTNV nucleocapsid protein (NP) elicited by virus infection or DNA vaccination to investigate the immunogenicity of NP. To examine the cellular immune response against HTNV NP, C57BL/6 mice were injected with HTNV intraperitoneal. The NP-specific CD8+ T …
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MOLECULAR MECHANISMS OF ATP-MODULATED LIQUID-LIQUID PHASE SEPARATION (LLPS) OF TDP-43 AND SARS-COV-2 NUCLEOCAPSID PROTEIN
… to behave as a biohydrotrope to prevent protein LLPS. However, a high-resolution mechanism for ATP-modulated LLPS has been lacking so far. Here we included TDP-43 and SARS-CoV-2 N proteins as two paradigms to decipher how ATP regulates protein LLPS. The results revealed that ATP …
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Computational studies of biomolecules to explore potential therapeutics for COVID-19 and SAMD9- associated diseases
… public health. Various therapeutics that target proteins of the virus have been studied, which have shown some degree of success. Advancements in computational methods have offered an excellent opportunity to explore biological macromolecule conformations, functions, and pathogenesis. Therefore, …
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Super-resolved volumetric optical imaging of virus-infected cells
… SARS-CoV-2 infected Vero cells revealed that the nucleocapsid protein of SARS-CoV-2 accumulates at the membrane of the viral replication organelles, strongly suggesting that this is where the encapsidation of the viral genome by the nucleocapsid protein occurs. Imaging the assembly of the viral …
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The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants
… involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its …
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Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum
… The construct contained GFP (green fluorescent protein) and hygromycin antibiotic-resistant selectable markers, and the antisense sequence of open reading frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene silencing. The presence …
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T-cell Dysfunction by HCV Core Protein Involves PD-1/PD-L1 Signaling.
… donors when exposed to extracellular HCV core protein (a nucleocapsid protein that is immunosuppressive); upregulation of PD-1 is mediated by core's ability to bind to the complement receptor gC1q. We also report that the observed T-cell function can be restored by blocking the PD-1/PDL-1 …
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Targeting RNA With Small Molecules: SL3 of HIV -1 Psi-Rna
… determinant through its interaction with viral nucleocapsid protein, NCp7. Since SL3 is not a target of current anti-HIV therapeutics, the discovery of small molecules that target SL3 would not only expand the understanding of RNA recognition by small molecules, but could also lead to discovery …
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Applications of Engineered Proteins in Redox Biology and Biomarker Detection Assay Development
Engineered proteins are very versatile tools that have been applied in assay development for various purposes. They have been made into genetically encoded biosensors/probes or affinity agents for biomarker detection. This thesis explored a few topics using assays developed with engineered …
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Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions
… Evidence indicates that the major envelope protein, GP5, is the primary target for a subunit vaccine. In native virions GP5 primarily exists as a disulfide linked complex with the membrane protein, M, which also possesses immunogenic properties. Recent studies report that the GP5/M complex …
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Molecular Sensing with Protein and Solid-State Nanopores
… of biological interest, such as DNA, RNA and proteins. Of particular appeal is this technique's ability to probe these molecules without the need for chemical modification or labeling, to do so at physiological conditions, and to probe single molecules at a time, allowing the possibility for …
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Development of a Fluorescence Microsphere Immunoassay for Detection of PRRSV Infection Using Oral Fluid Samples as an Alternative to Serum-based Assays
… in oral fluid and serum samples. Recombinant nucleocapsid protein (N) and nonstructural protein 7 (nsp7) from both PRRSV genotypes (Type I and Type II) were used as antigen and covalently coupled to Luminex fluorescent microspheres. Based on an evaluation of 488 oral fluid samples with known …
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The development of novel diagnostic countermeasures for Rift Valley fever virus
… of antibodies against a RVFV surface glycoprotein and the nucleocapsid protein. The targets developed in this assay provide the basis for a DIVA-compatible serological assay with a candidate RVFV Gn/Gc subunit vaccine, as well as, offer a multiplexing platform that can simultaneously screen …
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Genetic diversity and taxonomical relationships among the tacaribe serocomplex viruses (family arenaviridae)
… of the amino acid sequences of the glycoprotein precursors and nucleocapsid proteins of 70 arenaviruses, representing the 22 species in the Arenaviridae, indicated that the minimum nonidentity between strains of different species was 15.8% and 11.2%, respectively. Thus, arenaviruses that …
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Investigating the mechanism of RNA packaging in Rift Valley Fever phlebovirus
… S segments. The virion carries two envelope glycoproteins, Gn and Gc, along with ribonucleoprotein complexes (RNPs), composed of encapsidated genomes carrying the nucleocapsid protein, N, and the RNA-dependent RNA polymerase, L protein. Understanding the mechanisms that govern the recognition and …
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<i>Maize fine streak virus</i> (MFSV) gene expression and protein interaction
… RNA that encodes five core structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the polymerase (L), the matrix protein (M) and the glycoprotein (G) and two non-structural proteins, MFSV 3 and 4. The genes follow the order 3'-N-P-3-4-M-G-L-5’. Functions for the MFSV …
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Preliminary Analysis of Vital Proteins of the Human Respiratory Syncytial Virus
… Human Respiratory Syncytial Virus produces 10 proteins carrying out a diverse array of roles for the infectivity of the virus. Despite this there is still a poor understanding of the three dimensional structure. Only two proteins have been resolved to atomic resolution, the Nucleocapsid protein …
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