Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"Nucleic acid detection"”.
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CRISPR Biosensors for Resource-limited Nucleic Acid Detection
… new class of diagnostics with sequence-specific nucleic acid detection capabilities that can provide a rapid response without the need for traditional laboratory infrastructure. The research presented in this dissertation aims to further characterize and expand the applicability of CRISPR-based …
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Dual parametric sensors for highly sensitive nucleic acid detection
… for the highly sensitive and label-free detection of Nucleic Acid hybridization. These novel capacitive sensors with nano-scale electrode spacing offer solutions to many problems suffered by the conventional signal transduction mechanisms, thereby immensely improving the sensitivity of …
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A versatile protein platform for sequence specific nucleic acid detection
Detection and quantification of genetic material is fundamental for many research and diagnostic applications. Many different methods has been developed towards this objective, where each is typically tailored to detect a specific types of nucleic acid target under defined conditions. We have …
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Point of care platforms for protein and nucleic acid detection
… is developed and validated first for the detection of IL-6. The assay principles are further used to develop microbead based detection for PCT. The final platform demonstrates the simultaneous detection of both IL-6 and PCT from human plasma samples. Aside from utilizing microfluidics for …
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Advanced nucleic acid detection with solid-state nanopores: from fundamental understanding to cutting-edge platforms
… as a powerful technique for single molecule detection, due to its operational simplicity, high sensitivity, and real-time analytical capability. Despite these advantages, significant challenges remain in achieving multiplexed detection of diverse targets and in enabling precise molecular …
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Development of Universal Biosensing Platforms Based on CRISPR/Cas12a systems
… and cost-effective method for diverse target detections. This thesis focusses on the development of CRISP/Cas12a based biosensing platforms for nucleic acid and protein detection. Two distinct CRISPR/Cas based diagnostic methods were developed. The first developed method is a plasmonic CRISPR …
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Modular CRISPR-diagnostics for Infectious Diseases
… I present two novel approaches for multiplexed nucleic acid detection (CARMEN and WATSON) that build on the existing CRISPR-diagnostic method SHERLOCK. SHERLOCK combines traditional amplification (PCR or isothermal) with target-specific CRISPR-Cas13 detection and enables new, modular assay …
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Designing Next Generation Nucleic Acid Diagnostics Using Synthetic Biology and Artificial Intelligence
Nucleic Acid Testing (NAT) is an indispensable tool for effective disease diagnosis. Analyzing pathogen and host RNA or DNA often provides otherwise unobtainable information necessary for proper patient treatment. Unfortunately, a number of technical barriers prevent the expansion of NAT technology …
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Cell-free synthetic biology for affordable, on-demand diagnostics
Detection of biomarkers, such as nucleic acids, performs critical roles in managing infectious disease outbreaks, point-of-care testing, and public health monitoring. However, many diseases and public health problems suffer from a lack of affordable, portable tests that can be used to sensitively …
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Single-molecule detection of molecular beacons generated from LDR on thermoplastic microfluidic device for bioanalysis
Current clinical techniques for nucleic acid detection and analysis often involve PCR, lacking adequate specificity or sensitivity to meet the stringent requirements in certain applications. This research aims to develop an innovative molecular assay and the associated hardware to rapidly signal …
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Technical evaluation of a Real-time polymerase chain reaction (PCR) assay for the detection of Bartonella spp for diagnostic purposes
… and largely based on the combination of nucleic acid detection methods and serological investigation. There is a paucity of published data on microbes causing culture-negative endocarditis, but a recent report indicated that the bacterium Bartonella was the commonest cause of …
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Characterization and Engineering of Transposons for Genome Editing
… of this thesis, we develop a new chemistry for detection of SARS-CoV-2 called STOPCovid to address the urgent need for diagnostics in the COVID-19 pandemic. STOPCovid aims to simplify liquid handling for sensitive nucleic acid detection in low-complexity and point-of-care settings. We …
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Luminescence-Based MicroRNA Detection Methods
… be able to detect these small molecules. Current detection methods of Northern blotting, microarrays, and qRT-PCR suffer from drawbacks including low sensitivity, a lack of simplicity, being semi-quantitative in nature, time-consuming, and requiring expensive instruments. This work aims to develop …
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From Sample to Answer: Innovations in sample processing and CRISPR-based diagnostics for enhanced clinical translation and field deployment
… current diagnostic gap, but recent advances in nucleic acid-based technologies, and CRISPR-based diagnostics (CRISPR-Dx) in particular, have shown significant promise in transforming infectious disease detection. CRISPR-Dx are easily programmable, robust, sensitive, isothermal, and highly …
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Engineering programmable nucleases for in vivo and in vitro applications
… not only for genome editing but also for nucleic acid detection, offering highly specific and sensitive diagnostic tools. The primary focus of my dissertation is to engineer these programmable nucleases for both in vitro and in vivo applications using synthetic biology approaches. In the …
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DEVELOPMENT OF E-SRS (ENVIRONMENT-SENSING RESPONSE SYSTEM) AS A NOVEL METHOD TO DISTINGUISH GENETIC ENVIRONMENTS AND RESOLVE CLOSELY RELATED NUCLEIC ACID SEQUENCES
Existing limitations of conventional Nucleic Acid (NA) detection prompted us to conduct a Proof-of-Concept of a novel NA sensing platform called environment-Sensing Response System (e-SRS), which could deliver a physical response upon sensing its NA Target Sequence (NTS). e-SRS is a NA sensing and …
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CRISPR-based approaches for the detection of antimicrobial-resistant genes
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-05-01
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Point-of-care biosensing for rapid and precise biomolecular diagnostics
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2026-05-01
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A Binary Approach for Selective Recognition of Nucleic Acids and Proteins
… for the selective recognition of biopolymers (nucleic acids and proteins) is a fundamental task for studying, diagnosing, and treating diseases. Traditional methods utilize a single component (small molecule or oligonucleotide) that binds directly to the target biopolymer. However, many …
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