Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Nucleic acid analysis"”.
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Solid-state nanopore sensors for nucleic acid analysis
Nanopore DNA analysis is an emerging technique that involves electrophoretically driving DNA molecules through a nano-scale pore in solution and monitoring the corresponding change in ionic pore current. This versatile approach permits the label-free, amplification-free analysis of charged polymers …
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The utility of modified nucleotides for high-throughput nucleic acid analysis /
Synthetic base-modified nucleic acids are widely used in various fields. The aim of this dissertation was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for …
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A modular approach to high throughput microsystems
… developed using microfabrication technology and nucleic acid analysis. The purpose of developing this microsystem is to identify acute infectious disease and prevent contagious outbreak in a matter of time. This microsystem included three major components, a reagent distribution device, a 96 …
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Modifikuotų nukleotidų taikymas plataus masto nukleorūgščių analizei /
Synthetic base-modified nucleic acids are widely used in various fields. The aim of this dissertation was was to investigate the properties and utility of oligonucleotide-tethered 2′,3′-dideoxyribonucleoside 5′-triphosphates (OTDDNs), with emphasis on labeling of DNA and cDNA molecules for …
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In situ formed magnetic ionic liquids: DNA extraction performance and fluorescence-compatibility in bioanalytical applications
… a significant sample preparation challenge in nucleic acid analysis. Current methodologies such as the phenol-chloroform extraction use toxic organic solvents and commercially available kits are often very expensive and have limited reusability. Magnetic ionic liquids (MILs) have gained …
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Comparison of nucleoproteins and nucleic acids of five isolates of tomato ringspot virus
… into three strains by nucleoprotein and nucleic acid analysis. A grape isolate from one location (GSGF), two grape isolates from another location (GBNR, G4NR), a seed-borne soybean isolate (S1B), and a dandelion isolate (D4WR) from the second location were compared. The three grape …
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
<p>Sample preparation is vital in nucleic acid analysis. Cell lysis is generally the first step in DNA sample preparation where the cell membrane is disrupted to release intracellular components such as DNA. Moreover, the isolation and enrichment of nucleic acids is essential to prevent false …
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Ultrasensitive methods for the detection of biomolecular systems at the micro/nano scale
… DNA sequences detection, due to the relevance of nucleic acid analysis in many areas, including clinical diagnostics, environmental monitoring and food-quality control. However, diagnostic tools used for the detection of nucleic acids need to fulfil specific requirements in term of sensitivity, …
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Advanced nucleic acid detection with solid-state nanopores: from fundamental understanding to cutting-edge platforms
… in glass nanopore sensing for both DNA and RNA analysis by leveraging nucleic acid self-assembly, all without requiring enzymatic amplification or fluorescence labelling. To establish a fundamental understanding of signal generation mechanisms, I first conducted a systematic investigation into …
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A Binary Approach for Selective Recognition of Nucleic Acids and Proteins
… for the selective recognition of biopolymers (nucleic acids and proteins) is a fundamental task for studying, diagnosing, and treating diseases. Traditional methods utilize a single component (small molecule or oligonucleotide) that binds directly to the target biopolymer. However, many …