Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Nucleic Acid-based"”.
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Developing Nucleic Acid-Based Sensors and Actuators
… of regulatory logic in complex environments. Nucleic acids are attractive molecules for designing sense-and-respond modules: they are ubiquitous, information-rich and interact with each other through simple rules. Here, through two examples, I show that nucleic acids are particularly suited to …
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A nucleic acid-based bacterial message export system for cell-to-cell communication
… funneled from the sender to the receiver cell. Nucleic acids are rarely used as extracellular messengers due to their labile nature and proclivity for enzymatic digestion. Eliminating these obstacles will allow for a larger array of messages to be sent with minimal cellular machinery. Exploiting …
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Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli
… oligonucleotide ligation-PCR (MOL- PCR) is a nucleic acid based assay patented at Los Alamos National Laboratory (LANL) that uses flow cytometry and multiplex microsphere arrays for detection of nucleic acid based signatures. By using MOL-PCR for detecting unique STEC DNA signatures in samples …
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High sensitivity pathogen detection assays from crude samples
… and fungal detection and immunoassay or nucleic acid-based testing for viral detection, these approaches are time-consuming, expensive, and require extensive resources and time for the identification of pathogen. Standard protocols for nucleic acid-based detection for pathogens require …
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Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function
… Macromolecular drugs, and in particular nucleic acid based drugs, offer a potential remedy for this situation. Currently, the absence of efficacious and safe methods to deliver nucleic acids to intracellular sites of action is the main impediment to the introduction of nucleic acid based …
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Development of Functional DNA -Based Sensors and Investigations Into Their Mechanism
… the development of a successful functional nucleic acid-based sensor is its isolation and this is done through in vitro selection. In vitro selection to isolate DNAzymes for Hg2+ and Cd2+ and the use of negative selections to overcome Pb 2+ interference at various stages of selection has …
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Insights from biomolecular condensates into disease and drug development
… concentrates and compartmentalizes proteins and nucleic acids into diverse phase-separated biomolecular condensates. The study of condensates has yielded myriad fundamental insights into cell biology, ranging from a better understanding of cellular organization, to the exploration of novel …
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Aptamers that bind filovirus GP : a new frontier for detection and neutralization of Ebola and Marburg virus
Aptamers are single chained, nucleic acid-based affinity probes that bind to their targets with strong affinity and specificity. They are made through an in vitro combinatorial selection method, wherein large libraries of nucleic acids with randomized sequences are subjected to an iterative process …
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Production, characterization and evaluation of the chemotherapeutic and immunogenic potential of protein based virus-like particles in tumoral and immune cell lines
… systems. Vaccine platforms. Protein-based particles. Nucleic acid-based particles. Particle decoration. Doxorubicin. Cancer.
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Development of Carbohydrate-based Diblock Polymers for Nucleic Acid Delivery
The delivery of nucleic acids remains the major obstacle for nucleic acid-based therapies such as gene therapy and gene silencing therapies based on RNA interference. In this dissertation we have developed and studied nucleic acid delivery vehicles based on cationic diblock glycopolymers that …
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Granular Hydrogels for Localized Nanoparticle Delivery
… advanced therapeutics remains a major challenge. Nucleic acid–based therapies hold enormous promise for locally modulating gene expression and restoring skin homeostasis, but their translation is limited, among others, by the lack of simple, adaptable, and clinically relevant delivery platforms. …
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Development of an oral DNA anti-cancer vaccine targeting an angiogenic peptide, proadrenomedullin N-terminal 20 peptide (PAMP)
Nucleic acid-based anticancer vaccines are becoming a very active field in the fight against cancer. The goal of this thesis was to generate an oral DNA vaccine targeting the angiogenic peptide, proadrenomedullin N-terminal 20 peptide (PAMP). Methods: An expression plasmid (PcPAMP) was generated by …
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Using Chemically Modified Oligonucleotides to Modulate Gene Expression, Treat Genetic Diseases, and Probe Novel Mechanisms of RNA Interference
… interesting and therapeutically promising nucleic-acid-based compounds that could open new doors to finding a cure for genetic diseases such as HD.
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Cross-talk-free dual-color fluorescence cross-correlation spectroscopy for high-throughtput screening
… of proteins (thrombin) at the SMD level. Nucleic acid-based fluorescence sensors were used as recognition elements for the detection of single protein molecules with single-pair fluorescence resonance energy transfer. The technique provided higher analytical sensitivity compared to bulk …
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Computational Approaches to Detect Pathogens in the Presence of Complex Backgrounds
… sensitive and accurate detection approaches are based on the examination of the nucleic acid composition of the sample in order to identify the presence of pathogens DNA and/or RNA. A large spectrum of nucleic acid-based tests (such as PCR, RT-PCR, and oligonucleotide microarrays) is designed to …
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The conjugation and evaluation of hydrophobic small molecules to trazole-linked siRNAs
… for destruction. To overcome these limitations, nucleic acid-based therapies, such as short interfering RNAs (siRNAs), have been developed to aid in the regulation of proteins. SiRNAs serve as substrates for the highly conserved RNA interference (RNAi) pathway, which gives rise to their high …
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Inibizione selettiva del gene MYCN mediante PNA (acidi peptido nucleici) anti-gene nel rabdomiosarcoma umano
… therapy. PNAs belong to the latest class of nucleic acid-based therapeutics, and they can bind chromosomal DNA and block gene transcription (anti-gene activity). We have developed an anti-gene PNA that targets specifically the MYCN gene to block its transcription. We report for the first time …
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From Sample to Answer: Innovations in sample processing and CRISPR-based diagnostics for enhanced clinical translation and field deployment
… current diagnostic gap, but recent advances in nucleic acid-based technologies, and CRISPR-based diagnostics (CRISPR-Dx) in particular, have shown significant promise in transforming infectious disease detection. CRISPR-Dx are easily programmable, robust, sensitive, isothermal, and highly …
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Lipid Biomarkers for Marine Fungi
… of starvation on intact polar lipids and fatty acids in the marine yeast Aureobasidium pullulans, a saprotroph found throughout the global oceans. The results indicated that starvation induced rapid phospholipid degradation and increased the production of exophilins. In addition, fatty acids …
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Development of functional DNA-based sensors and investigations into their mechanism
The discovery that nucleic acids could perform functional roles in addition to being genetic materials carriers opened doors to a new paradigm in nucleic acid chemistry. Catalytic DNA molecules also known as deoxyribozymes or DNAzymes were first isolated in 1994 through an in vitro selection …
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