Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 188 for “"Nuclease"”.
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Toward the Synthesis of Nuclease Models.
<p>Nucleases are enzymes that can specifically recognize nucleic acids and hydrolyze their phosphodiester bonds effectively. As is the case with many hydrolases, nucleases often carry one or more metal centers. Cooperation between such metal centers and other interactions involving general …
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The Apollo Nuclease Binds To TRF2 and Protects
<p>Members of the β-CASP family of proteins are involved in DNA repair and RNA processing. We identified a member of this family of enzymes, hSNM1B/Apollo, in complex with the telomere binding proteins TRF2/Rap1. Due to its low abundance at telomeres, we consider Apollo to be a telomere accessory …
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Crystalline Protein Dynamics : A Simulation Analysis of Staphylococcal Nuclease
… (MD) simulations of crystalline Staphylococcal nuclease are reported and analysed in terms of fluctuations, correlations, X-ray diffuse scattering, disorder and models of protein motion. Convergence properties of dynamical quantities are determined. The logarithmic dependence on the simulation …
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The Transposon-Encoded Protein TnpB is an RNA-Guided Nuclease
… One CRISPR effector protein, the Cas12 nuclease, is hypothesized to have its evolutionary origins in TnpB, an abundant transposon-encoded protein that contains a predicted nuclease domain. The biochemical properties of TnpB are unknown, but may provide clues to the evolutionary origins …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… has been achieved by the application of designer nuclease technologies, where engineered DNA binding domains, consisting of either a dimeric zinc finger protein (ZFP) or a transcription activator-like effector (TALE) domain, is conjugated to a dimeric nuclease domain, producing zinc finger …
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Release of Histone Lysine Methyltransferases from Rat Brain Chromatin by Nuclease Digestion
… rat brain chromatin with micrococcal nuclease. The enzymes were further purified by gel filtration, ammonium sulfate fractionation and DEAE-cellulose chromatography. The histone methyltransferases were resolved into two distinct fractions by Sepharose 6B-100 and DEAE-cellulose …
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Structure, Mechanism and Chemical Modulation of the Protein Kinase-nuclease Dual-enzyme IRE1
… eliminate an inhibitory intron using a dormant nuclease function in its cytoplasmic effector region (IRE1cyto). My focus was to understand the mechanism of XBP1/HAC1 activation by IRE1, the regulation of IRE1 function and the manipulation of IRE1 signaling output using chemical tools. To better …
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Nuclease-Mediated Targeted Gene Insertion at the Adenosine Deaminase Locus in Primary Cells
… end, we developed several pairs of TAL effector nucleases (TALENs) designed to target exon 1 of ADA. These TALENs function as dimers, and each pair creates a different targeted double strand break near the start site of the ADA gene. The most effective pair induces a DNA double strand break …
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New Insights into the Roles of FAN1 Nuclease in Genome Maintenance and Disease
… genotoxic threats. FANCD2 and FANCI associated nuclease 1 (FAN1) has roles in protection against two major threats to genomic stability: DNA interstrand crosslinks (ICLs), and the expansion of trinucleotide repeats. ICLs are highly deleterious lesions that disrupt replication, are destructive to …
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Analysis of the Structure and Cell-Specific Transcription of the Bovine Parathyroid Hormone Gene (Micrococcal Nuclease)
Two EcoR I restriction fragments containing PTH gene sequences were isolated from a partial bovine library prepared using EcoR I cleaved high molecular weight bovine DNA and lambda phage charon 30 as a cloning vector. Restriction and sequence analyses of the two fragments indicate that they are …
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ELECTROPHORETIC AND ENZYMATIC IDENTIFICATION OF PROTEINS ASSOCIATED WITH THE DEVELOPMENT OF COMPETENCE IN BACILLUS SUBTILIS
… zymogram procedure for the analysis of nucleases is described. Using purified deoxyribonuclease I (bovine pancreas), as little as 10 pg of nuclease can be detected. Isoelectric focusing (IEF) and non-equilibrium pH gradient electrophoresis (NEPHGE) were compared as first dimensions, in …
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Deoxyribonuclease probing on sea urchin embryo chromatin
… and in developing embryos using micrococcal nuclease, pancreatic deoxyribonuclease (DNase I) and restriction endonucleases. Micrococcal nuclease and restriction endonuclease digestions of sea urchin gastrula chromatin have been analysed and it is shown that it is not possible to isolate large …
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Evaluation of the Protein Recognition Properties of Peptide Nucleic Acids
… due to its increased thermostability and nuclease resistance over natural nucleic acids. PNA represents a superior alternative to natural nucleic acids in many biomedical applications due to its specificity, strong binding, and nuclease resistance. This study is the initial data set that …
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Repair eXtreme - DNA repair proteins from Antarctic extremophiles
… interesting fusion with an N-terminally located nuclease domain, which resembles an extensively studied MBL-β-CASP domain found in all domains of life. The recombinantly expressed nuclease domain can coordinate several types of metal ions and shows nuclease activity against a diverse range of DNA …
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Advancing and expanding siRNA and saRNA therapeutics applications through chemical modifications
… treatment. The third goal focused on discovering nuclease-resistant and active saRNAs targeting STING, which is a potential target for the treatment of solid tumors. In this study, a library of chemically modified saRNA was screened for their nuclease resistance ability and investigated for any …
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Characterisation of the Type VII secretion system of <i>Staphylococcus aureus</i>
… predictions suggested that EsaD has a nuclease domain at its C-terminus and in support of this EsaD was shown to lead to DNA damage when produced in E. coli. in vitro nuclease assays using the purified EsaD C-terminal domain confirmed that it has Mg2+-dependent DNase activity. EsaG, …
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Biochemical and Structural Studies of the Enzymes Involved in RNA Cleavage and Modification
… of 16S rRNA. Colicin E3 is a highly specific endonuclease that cleaves a single bond in 16S rRNA between A1493 and G1494. This cleavage results in termination of protein synthesis in the cell. In order to determine whether the nucleotide sequence of the target site accounts for colicin E3's …
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