Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 13 of 13 for “"Nuclear speckles"”.
-
Genomic and microscopic dissection of nuclear compartmentalization and function
… for gene expression regulation. Abnormal nuclear organization is related to disease. Microscopy measurements of individual genes reveal a population-based, statistical gradient of increased gene activity from the nuclear periphery to the center. Sequencing-based methods (Hi-C, DamID) …
-
Nuclear speckle dynamics and function of speckle association in gene expression
Nuclear speckles have been studied intensively for a long time. Accumulating evidence has suggested that nuclear speckles have a dynamic structure, and are associated with a significant fraction of active chromosome regions and genes. However, their mobility and the regulation mechanism of their …
-
"""TSA-Seq"": a novel proximity mapping approach for studying three dimensional genome organization and function"
… and distribution in the three dimensional (3D) nuclear space, and the inability to connect three dimensional microscopic information with the linear genome sequence. In this dissertation, I describe ""TSA-Seq"", a novel proximity mapping approach I have developed to study 3D genome organization. …
-
Mechanisms and functional significance of nuclear compartmentalization
… For better understanding of the effects of nuclear repositioning of a gene locus on gene expression, I investigated the physiological significance and mechanism of association of a gene locus with a nuclear sub-compartment. In chapter 1, I showed an actin dependent association of HSP70 …
-
Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… loci are located closely to each other in the nuclear compartment enriched by the nuclear speckles. However, in KtyII locus knockout mice, EDC locus moved away from the KtyII locus flanking regions and nuclear speckles towards the nuclear periphery, which is associated with marked changes in …
-
Investigating Mechanisms of Nuclear mRNA Export
… in the cytoplasm has important implications for nuclear export and retention of mRNAs. This division ensures that only correctly processed mRNAs are exported to the cytoplasm to be translated into proteins, while misprocessed and aberrant transcripts are retained in the nucleus to be degraded by …
-
Development of Oligonucleotide-directed proximity-interactome MAPping (O-MAP), for characterizing RNA-protein interactions and higher order subnuclear architecture in situ
… local subcellular environments and assembling subnuclear bodies. A classic example of RNA-scaffolded structures is the nucleolus, a subnuclear body that serves as the site of ribosome biogenesis, cell cycle regulation, and stress responses. Nucleolar assembly is initiated de novo after each cell …
-
Nova Regulation of Alternative Splicing in the CNS
… and CNS motor dysfunction, is a neuronspecific nuclear RNA binding protein. The work presented here confirms that Nova-1 acts as a regulator of alternative splicing in vivo. In addition to the known target Nova-dependent splicing regulation, exon 3A of glycine receptor α2 subunit (GlyRα2), two …
-
Identification and functional characterization of the novel MAR-binding protein, SATB2
… complex. In addition to enhancers and promoters, nuclear matrix attachment regions (MARs) have been implicated in the regulation of gene expression by altering the organization of eukaryotic chromosomes and augmenting the potential of enhancers to act over large distances. Although a lot is known …
-
SPAG16 is a Bifunctional Gene Regulating Male Fertility
… within the nucleus of round spermatids. These nuclear sub-domains also contain SC35, a known marker of nuclear speckles enriched in pre-mRNA splicing factors. Putative interaction partners of SPAG16S are also shown to play critical roles in the peri-nuclear region during the round spermatid …
-
Isolation and Functional Mapping of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein DNA-Damage Complexes
… and co-localizes with phosphor-DNA-PKcs within nuclear Tax Speckled Structures (TSS). Cytoplasmically-localized Tax deletion mutants cause a redistribution of phosphor-DNA-PK to the cytoplasm. Tax-expressing cells harbor significantly increased DNA-PK kinase activity, as measured in an <em>in …
-
Advancements in genome engineering and applications in mammalian systems
… mediated loci labeling methods. Signal to nuclear noise ratios (SNRs) were analyzed first. The high SNRs indicated all methods should be suitable for live cell imaging applications. Labeled loci positioning to nuclear speckles were found similar among these methods. Orthogonal imaging of …
-
New insights into 3D genome architecture through improved genomic methods
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01