Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Nuclear shape"”.
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Synaptic and extrasynaptic NMDA receptors in hippocampal neurons: regulation of nuclear shape and cell fate
… calcium signal that invades the cell nucleus. Nuclear calcium is a key regulator of gene expression mediated by the transcription factor CREB. The nuclear architecture contains intranuclear structures that have been shown to facilitate calcium influx into the nucleus. This thesis describes a …
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HeLa에서 K-ras 과발현시 활성산소 발생에 의한 핵모양의 이상의 발생과 이중핵/다핵 세포 증가 현상 연구
Polyploidy and abnormal nuclear shapes are well known characteristics of cancer cells. K-Ras is frequently mutated in many cancers. One of the oncogene mutants, K-RasV12 has been reported to generate reactive oxygen species (ROS). We recently observed that increased ROS generates polyploidy and …
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Rigidity in Frictional Particle Packings and Dynamics in Living Polymeric Packings
… to drive correlated chromatin dynamics and nuclear shape fluctuations. To test this notion, we develop a minimalistic model in which an active, crosslinked Rouse chain linked to a polymeric shell. System-scale correlated motion occurs and requires both motor activity and crosslinks. …
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Xenopus DPPA2 is a Direct Inhibitor of Microtubule Polymerization Required for Nuclear Assembly
… open mitosis as well as during fertilization. Nuclear abnormalities are observed in many tissue malignancies and congenital disorders, but the causes and effects of such pathologies remain poorly understood. Here we use cell-free Xenopus egg extracts to investigate the contribution of the …
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Lessons from a rare disease
… lamins that comprise the inner lining of the nuclear wall. The mutation leads to the production of a misshapen lamin called progerin that builds up with time, disrupting nuclear shape and function. It is not yet clear how these changes lead to the disease's symptoms. Doctors probe potential …
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Ab Initio Nuclear Structure Calculations for Light Nuclei
… issues and better represent features of the nuclear shape. Convergence</p> <p>properties of these density distributions shed light on the convergence properties of experimental</p> <p>one-body observables.</p> <p>We then present a method to calculate the space-fixed and …
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Quantitative phase imaging : a study from 2D to 3D
… on the one hand can retrieve morphological shapes, and on the other hand can be converted to dry mass values, which are important bio-markers for cell growth studies. By adapting QPI system into an image cytometer, termed Quantitative phase cytometer (QPC), a large population of ~ 10⁴ HeLa …
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Developing a User-Friendly and Modular Framework for Deep Learning Methods in 3D Bioimage Segmentation
… as the object of interest to understand how its shape variations contribute to the organisation of the genetic material. First a literature survey showed that very few publicly available methods for 3D nucleus segmentation provide the minimum requirements for their reproducibility. These methods …
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The Impact of substrate texture on cell behavior
… measurements showed that the cell thickness and nuclear shape is regulated through the different arrangement of perinuclear actin stress fibers depending on the stripe sizes. We also observed that when the size of stripes (5 µm) is significantly smaller than the size of cell nuclei (subnuclear …
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Signature splitting and non-axiality in the nuclei ¹⁶⁴Ta and ¹⁶⁵Ta
… substantial deviation from an axially symmetric shape is explored. In addition a weaker excited band, possibly associated with the π[402]5/2⁺ configuration, has also been identified. High-spin states in ¹⁶⁴Ta ( Z = 73, N = 91) were populated in the first experiment. The yrast band, identified up …
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Search for Collective Strutures in the 186Os And 150Gd Nuclei
… It is still one of the major challenges innuclear structure physics to properly understand the nature of these states. To givemore insight on the underlying nature of such states, the excited bands in186Os and150Gd nuclei were investigated at iThemba laboratory for accelerator based …
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NOVEL MECHANISMS IN THE REGULATION OF PERINUCLEAR ACTIN ASSEMBLY
… critical for cells? functions and survival. Perinuclear actin contributes to maintaining of nuclear shape and cellular mechanical homeostasis, and integrating cell nucleus into the actin cytoskeleton architecture. Actin dynamics is regulated by a number of different factors in concert. It is …
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Mechanisms of epigenetic regulation in epidermal keratinocytes during skin development. Role of p63 transcription factor in the establishment of lineage-specific gene expression programs in keratinocytes via regulation of nuclear envelope-associated genes and Polycomb chromatin remodelling factors.
… mice about 32% of keratinocytes showed altered nuclear morphology. Alterations in the nuclear shape were accompanied by decreased expression of nuclear lamins (Lamin A/C and Lamin B1), proteins of the LINC complex (Sun-1, nesprin-2/3) and Plectin. Plectin links components of the nuclear envelope …
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The Application of a Statistical Model Investigating Reactive Oxygen Species in Premature Ageing Syndromes
… the accumulation of immature prelamin A at the nuclear membrane. As a result of increased understanding of the defects in HGPS arising from LMNA mutations a number of therapeutic routes are currently being trialled, but with little success. Work in my supervisor’s laboratory has suggested that …
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Identifying novel roles for basement membrane-associated proteins in the adult Drosophila intestinal epithelium
… intestinal stem cells develop large nuclear lamina invaginations, nuclear shape changes and acquire DNA damage. Ultimately, the vast majority of Syndecan-depleted intestinal stem cells are lost from the epithelium, via a combination of apoptosis and other mechanisms. My work found …
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Dynamic organization of chromosomes in the mammalian cell nucleus
Uncovering the motifs of a higher order nuclear architecture and its implications on nuclear function has raised increasing interest in the past decade. The nucleus of higher eukaryotes is considered to display a highly dynamic interaction of DNA and protein factors. There is an emerging view that …