Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Nuclear organization"”.
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Novel Roles for A-Type Lamins in Maintaining Genomic Stability
… have been implicated in the maintenance of nuclear architecture and chromatin organization. Mutations in A-type lamins have been associated with defects in a number of nuclear processes, including DNA replication and repair, and gene transcription and silencing. In support of an important …
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Novel Single Cell Methods to Identify the Genetic Composition at a Single Nuclear Structure
… or the ligation of oligo probes to subnuclear sites of double stranded breaks induced by 2-photon irradiation. It is demonstrated that these techniques afford the ability to identify loci occupying a shared site at a single nuclear substructure, and are thus uniquely suited for …
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Genomic and microscopic dissection of nuclear compartmentalization and function
The spatial genome organization in the cell nucleus is non-random and important for gene expression regulation. Abnormal nuclear organization is related to disease. Microscopy measurements of individual genes reveal a population-based, statistical gradient of increased gene activity from the …
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A brain-derived MeCP2 complex supports a role for MeCP2 in RNA processing
… dependent. In addition, MeCP2 is involved in nuclear organization and proposed to regulate mRNA splicing. Mutations in MECP2 are linked to the severe postnatal neurodevelopmental disorder Rett Syndrome (RTT). To further understand MeCP2 and potential roles in RTT pathogenesis, we have employed …
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Myogenic Effectors and Disease
… show that constitutive and juvenile loss of the nuclear envelope protein Net39 in mice recapitulates different manifestations of Emery-Dreifuss muscular dystrophy. Deletion of Net39 caused disruption of nuclear envelope integrity and associated genomic, transcriptional, and metabolic changes that …
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APPROACHING DNA METHYLATION AT THE NANOSCALE
… modification, non-coding RNA, and nuclear organization. A fast-growing body of evidence suggests that epigenetic mechanism plays a fundamental role in physiology and pathology. Of the elucidated epigenetic processes, DNA methylation is the one under intense research due to its …
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Bioorthogonal Chemical Reporters Reveal Fatty-Acylation of Histone H3 Variants and Cholesterol Modification of Proteins and Trafficking in Cells
… processes from all cellular compartments. Many nuclear proteins were found, in particular histone H3 variants. Histones H3.1, H3.2 and H3.3 were demonstrated to be modified with fatty acid chemical reporters on the conserved cysteine 110, a novel site of S-acylation on histone H3.2. This newly …
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Nuclear speckle dynamics and function of speckle association in gene expression
Nuclear speckles have been studied intensively for a long time. Accumulating evidence has suggested that nuclear speckles have a dynamic structure, and are associated with a significant fraction of active chromosome regions and genes. However, their mobility and the regulation mechanism of their …
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Mechanisms and functional significance of nuclear compartmentalization
… is significant amount of knowledge about the organization of nucleus; however, the influence of such structural complexity of the nucleus in gene regulation is poorly understood. For better understanding of the effects of nuclear repositioning of a gene locus on gene expression, I investigated …
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"""TSA-Seq"": a novel proximity mapping approach for studying three dimensional genome organization and function"
"The organization of chromatin and its localization in the eukaryotic cell nucleus plays a critical role in gene regulation (Agarwal and Rao, 1998; Brown, 2003; Ferrai et al., 2010) and is important for maintaining normal genome function. However, although it is known that the distribution of …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
<p>In my thesis work, I explored the organization and dynamics of the nuclear pore complex (NPC) through coarse-grained modelling and polarized-total internal reflection fluorescence (pol-TIRF) microscopy. The NPC is a large (~120 MDa in humans) macromolecular assembly that controls the flow of …
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Mechanisms of epigenetic regulation in epidermal keratinocytes during skin development. Role of p63 transcription factor in the establishment of lineage-specific gene expression programs in keratinocytes via regulation of nuclear envelope-associated genes and Polycomb chromatin remodelling factors.
… mice about 32% of keratinocytes showed altered nuclear morphology. Alterations in the nuclear shape were accompanied by decreased expression of nuclear lamins (Lamin A/C and Lamin B1), proteins of the LINC complex (Sun-1, nesprin-2/3) and Plectin. Plectin links components of the nuclear envelope …
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Safety Culture Monitoring: A Management Approach for Assessing Nuclear Safety Culture Health Performance Utilizing Multiple-Criteria Decision Analysis
<p>Nuclear power plants are among the most technologically complex of all energy facilities. This complexity reflects the precision needed in design, maintenance and operations to harness the energy of the atom safely, reliably and economically. Nuclear energy thus requires consistent, high levels …