Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 62 for “"Nuclear membrane"”.
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Mechanisms of multidrug resistance in bladder cancer: the role of the nuclear membrane
… uptake and also demonstrate a<br/>characteristic nuclear sparing of drug uptake. This nuclear sparing<br/>phenomenon in MDR cells transcends tissue type and suggests that the<br/>nuclear membrane may also play a role in MDR.<br/><br/>The work described herein discusses the current role of …
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Characterizing the Role of Spegless, a Novel Inner Nuclear Membrane Protein required for spermatogenesis and oogenesis in Drosophila melanogaster
… mutations in the eye. Speg is a multi-pass transmembrane protein with a conserved domain of unknown function that I have confirmed is an inner nuclear membrane protein. I generated a null allele of speg and subsequent in depth analysis of speg null mutants revealed that Speg is essential for the …
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Pct1 regulates phosphatidylcholine synthesis in response to changes in surface curvature elastic stress sensed on the inner nuclear membrane
Cell and organelle membranes consist of a complex mixture of phospholipids that determine their size, shape, and function. Among the distinct types of phospholipids found in membranes of living organisms, phosphatidylcholine (PC) is the most abundant. The rate-limiting step of the predominant …
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Characterizing the Nuclear Function of the Cell Adhesion Molecule Fat and Chromatin Regulation by the Inner Nuclear Membrane Protein Speg
… of Ft in vivo. ChIP-seq results suggested that nuclear Ft binds mostly open chromatin. Interestingly, the ChIP-seq profile of Ft is highly correlated with that of the Hippo pathway transcription cofactor Yki. Overall, I propose that nuclear translocation of Ft is a novel mechanism by which Ft …
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The molecular basis of LINC complex formation
… cytoplasm occurs mostly through openings in the nuclear envelope composed of nuclear pore complexes. These massive assemblies allow for regulated transport of macromolecules across the barrier that is the nuclear envelope. However, another means of communication between the nucleus and the …
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Characterizing the Role of the Herpes Simplex Virus Type 2 UL21 Protein in Viral Nuclear Egress
… in the primary envelopment of capsids during nuclear egress, however the precise mechanism by which it does this is unclear. To investigate the role of UL21 in HSV-2 nuclear egress, we focused on events upstream of primary envelopment. First, we examined the role of UL21 in disrupting the …
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Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells
… replicating polyoma viral DNA (Py II*), a nuclear extraction technique is chosen and then slightly modified in order to rapidly yield purified nuclei in which the polyoma viral (Py) DNA is intact. A method is also developed which breaks the nuclear membrane, but not the Py DNA found within. …
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C. elegans apoptosis : CED-4 translocation and involvement in a model of mucolipidosis type IV human lysosomal storage disorder
… we found that CED-4 protein translocates to the nuclear membrane. In addition, inducing excess programmed cell death by expression of the EGL-1 cell-death activator triggers the translocation of CED-4 from mitochondria to the nuclear membrane. We performed a genetic screen for mutations that …
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Characterisation of Nuclear EnvelopeAssociated Proteins (NEAP) in Arabidopsis thaliana
… evolution, eukaryotic cells have acquired a nuclear envelope (NE) enclosing and protecting the genome, which is organized in chromatin, a structure wrapping DNA around histone proteins. The NE is composed of two membranes: on the nucleoplasmic side, the Inner Nuclear Membrane (INM) and on the …
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Synaptic and extrasynaptic NMDA receptors in hippocampal neurons: regulation of nuclear shape and cell fate
… calcium signal that invades the cell nucleus. Nuclear calcium is a key regulator of gene expression mediated by the transcription factor CREB. The nuclear architecture contains intranuclear structures that have been shown to facilitate calcium influx into the nucleus. This thesis describes a …
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Characterisation of the Arabidopsis mid-SUN proteins at the plant NE
… and Cytoskeleton (LINC) complex formation at the nuclear envelope (NE), similarly to Cter-SUN domain proteins. In addition to interacting with LINC complex components, they have been reported to localise to both the NE and endoplasmic reticulum (ER). There is also evidence that they interact with …
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Structural and mechanical properties of intermediate filaments under extreme conditions and disease
… association of intermediate filaments with the nuclear membrane, providing a molecular mechanism of the disease. We study the nuclear membrane association with intermediate filaments by focusing on the effect of calcium on the maturation process of lamin A. Our result shows that calcium plays a …
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The role of heterochromatin markers in lytic human herpesvirus egress and maturation
… remodeling as chromatin is marginalized to the nuclear periphery to accommodate viral replication and later to provide structural support during nuclear egress. However, the specific role of heterochromatin in these lytic infections remain unknown. In this thesis, I will show that host …
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Identification and Molecular Analysis of Dna In Exosomes
… DNA packaging into exosomes by disruption of the nuclear membrane. Finally, we investigated the ability of exosomes to induce paracrine DNA damage responses (DDR) in treatment-naïve cells. We explore the specificity of exosome-induced DDR to exosomes released by damaged cancer cells, and provide a …
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Influence of Kaempferol on Noncanonical DNA Structures and Protein Aggregation
… since KMP has been implicated in crossing the nuclear membrane as well as the blood brain barrier it is of interest to investigate for future natural product therapeutics. The current study found that KMP was able to effectively bind to G<sub>4</sub> and C<sub>4</sub> structures as well as …
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Deterministic Barcoding of Neuron Identities through Multicolor Fluorescent Markers in C. elegans
… tag subcellular markers of the nucleolus and nuclear membrane with fluorescent proteins to allow for a combinatorial barcode for neuron types, allowing the differentiation of neurons at a single-cell resolution. Through multicolor barcoding of individual neurons, we distinguish different cell …
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A quantitative characterisation of phospholipid composition and biosynthesis in HeLa cells and nuclei - a mass spectrometry study
… cells have been shown to contain a pool of endonuclear phospholipidthat is distinct from the nuclear membrane. It has been suggested that this endonuclear phospholipid is involved in the regulation of nuclear processes such as transcription. This study utilised tandem electrospray ionisation …
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Experimental and analytical techniques for studying mechanotransduction in articular cartilage
… calcium signaling, mitochondrial polarity, and nuclear membrane permeability. This thesis serves to outline the experimental and analytical methods developed to probe cellular response to external stimuli. While the focus is on method development, I will touch on some of the biological …
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Determination of the molecular mechanism of cell-to-cell movement in squash leaf curl geminivirus
… and must replicate in the nucleus. Thus, the nuclear membrane poses an additional barrier to cell-to-cell spread. To overcome this block, I propose that SqLCV encodes two MPs: a nuclear shuttle (BR1) to transport the ssDNA progeny as a BR1:ssDNA complex between the nuclear and cytoplasmic …
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