Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"Nuclear localisation"”.
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An investigation into the role of acetylation and ligand-dependent nuclear localisation in glucocorticoid receptor transcriptional regulation
The glucocorticoid receptor (GR) is a ligand-activated transcription factor which, due to its central role in anti-inflammatory responses, is a target of many therapeutically prescribed drugs. The GR undergoes multiple post-translational modifications, including phosphorylation and acetylation; …
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Subcellular localisation and functional analysis of UVR8, a UV-B specific signalling component in Arabidopsis
… spatial, temporal protein analysis, subcellular localisation studies, structure-function analyses, and the yeast-two-hybrid assay for the identification of UVR8 interacting proteins. To study spatial, temporal and wavelength specific UVR8 protein abundance anti-UVR8 peptide antibodies were …
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The characterisation of family-13 kinesins in Trypanosoma brucei
… during this study, only one was found to have a nuclear localisation, while the rest were found localised to the mitochondrion, cell body or flagellum. Attempts to probe the function of these kinesins using RNAi resulted in a reduction of cell growth in three of the six kinesins studied, but no …
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Modulation of West Nile Virus Capsid Protein and Viral RNA Interaction through Phosphorylation
… assembly processes like RNA binding; nuclear localisation and oligomerization were investigated. Phosphorylation of C protein attenuated RNA binding and this study showed that C protein was dephosphorylated later during infection to facilitate C protein and viral RNA interaction. It is …
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Solid Phase Synthesis and Biological studies on metal conjugates of bioactive peptides for targeted delivery
… affinities, efficient cellular uptake and/or nuclear targeting. Thus, in this research project, metal conjugates of some bioactive peptides were synthesized and biologically tested in order to evaluate the effects of these metal moieties on the biological activities such as the cellular …
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The effect of inhibiting KPNB1-mediated nuclear import on cancer cell biology and inflammatory transcription factor signalling
… in our laboratory and others identified the nuclear import protein, Karyopherin β1 (KPNB1), to be overexpressed in various cancers and that inhibiting its expression blocks the proliferation of cancer cells. However, little is known about the potential role of KPNB1 in other cancer cell …
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Dissecting the role of cathepsin V in breast cancer
… in the nucleus of luminal-A breast cancer cells. Nuclear localisation of cathepsin V appears to be cell-cycle dependent, with elevated levels detected during S and G2/M phases. Subsequent experiments suggest that cathepsin V contains a nuclear localisation signal, which is utilised for trafficking …
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Investigating the effects of enhanced catecholamine stimulation in stem cell-derived cardiomyocytes
… Isoprenaline resulted in an increased nuclear localization of the phosphorylated component of the TGF-β1 downstream intracellular mediator SMAD3 (p-SMAD3), compared to the peri-nuclear localisation of p-SMAD3 observed in the untreated and pindolol-treated cells. Furthermore, TGF-β1 …
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Characterisation of Nuclear EnvelopeAssociated Proteins (NEAP) in Arabidopsis thaliana
… evolution, eukaryotic cells have acquired a nuclear envelope (NE) enclosing and protecting the genome, which is organized in chromatin, a structure wrapping DNA around histone proteins. The NE is composed of two membranes: on the nucleoplasmic side, the Inner Nuclear Membrane (INM) and on the …
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Bone Morphogenetic Protein Signalling in Adult Lung Epithelial Cells
… was found to be modulated with the apparent relocalisation of the BMP receptor, BMPR-IA, to the nucleus of airway epithelial cells. Furthermore, this nuclear localisation was recapitulated in normal primary MAECs co-cultured with eosinophils or stimulated with eosinophil cationic proteins. …
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Phosphorylation of ERα and HIF-1α in breast cancer with focus on tamoxifen response and links to kinase activation
… kinase, of which high expression and particular nuclear localisation was associated with poor tamoxifen response. Two other biomarkers are different ER phosphorylations at serine 305 and serine 118; ERS305-P and ERS118-P, respectively. Tumours that were positive for ERS305 were associated with …
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Tracing clonal cell plasticity in the microenvironment of pancreatic ductal adenocarcinoma
… of epitopes attached to a fluorophore and a nuclear-localisation signal, which enables cell sorting, quantitative image analysis, and effective decoding by statistical analysis. Using subcutaneous mouse models of pancreatic cancer, this thesis demonstrates that isogenic epitope labelling can …
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Success and limitations of gene therapy with growth factors to enhance cutaneous wound healing
… were found to be of no use in skin tissue. A nuclear localisation signal effective in cell culture was also found to be of no value in vivo. The use of in mo elearoporation was found to hold the most promise for future skin gene therapy applications. Methods to improve in vivo transfection …
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Developing a novel Drosophila model to study Menin biological function
… However, the endogenous expression, subcellular localisation, and protein interactions of Drosophila Menin have not previously been characterised at the protein level. This work describes the generation and validation of a Drosophila line carrying an endogenous 3xHA-tagged allele of the principal …
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Investigating the role of histone H3 lysine 9 dimethylation in regulating disease-associated vascular smooth muscle cell gene expression
… did not influence the level of IL-1α-induced nuclear localisation of the NFkB transcription factor p65 but significantly increased IL-1α-induced p65 binding to the IL6 promoter, correlating with reduced H3K9me2 levels. In contrast, enrichment of p65 was not observed at reported NFkB sites …
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The Role of Hippo Signalling in Merlin Null Tumours of the Nervous System
… studies have shown that there is aberrant nuclear activity of yes-associated protein (YAP) and transcriptional coactivator with PDZbinding motif (TAZ), the co-transcriptional activators of the Hippo pathway in Merlinnull meningioma and schwannoma. Nuclear YAP and TAZ activate transcription, …
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Novel plant nuclear envelope-associated coiled-coil proteins
The nuclear envelope (NE) is a double lipid bilayer enclosing the eukaryotic genome. The metazoan nucleoskeleton includes the peripheral lamina and the internal nucleoskeleton. The lamina is composed of a network of intermediate filament (IF) proteins called lamins, as well as lamin- and/or …
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Dissecting the function of MYEF2 in neural stem cells and glioblastoma
… out in mouse NSCs. We observed that MYEF2 is a nuclear protein which retains nuclear localisation and expression in both proliferative and quiescence NSCs, as well as differentiating progeny. During mitosis, unlike SOX2, MYEF2 is not retained on the mitotic chromatin indicating it is not a …
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Investigation into the Molecular Biology of Human Genetic Sensory Disorders of Painlessness and Itch
… in the N-terminus of CMAS did not affect normal nuclear localisation nor the enzymatic function of the protein in sialylation. There were not any significant hits of interacting proteins found from the proteomic study. An interesting finding consistent with previous studies of CMAS is the fact …
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Targeting the N-myc oncoprotein using nanobody technology
… undruggable mainly due to challenges in nuclear drug delivery and physically targeting myc directly given that it is a largely disordered protein that lacks discernible clefts and pockets for small molecules to inhabit. Furthermore, c-myc is important in normal tissue maintenance so the …
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