Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"Nuclear entry"”.
-
A Structural and Biochemical Analysis of the Drosophila Protein Period
… production, posttranslational modification, and nuclear translocation of PER all contribute to the timing of the clock. This work describes the synthesis and purification of various Drosophila PER fragments for biochemical and crystallographic analysis. Several stable PER fragments are …
-
Discrete elements of the U2 snRNP regulate its intranuclear trafficking and recruitment to nascent transcripts
… elements of the U2 snRNP that control its intranuclear trafficking and association with nascent transcripts. Through a mutational analysis, I demonstrate that stem loops 3 and 4 (SL3 and SL4) are both required for U2 snRNP association with nascent transcripts and splicing speckles, while the Sm …
-
Elucidation of Design Criteria for Non-Viral Gene Delivery Vector Development
… carrier, migration of DNA to the nucleus, nuclear entry and finally transcription all stand as obstacles to gene delivery. The research presented in this thesis addresses the issue of gene delivery efficiency by studying vector dissociation and endocytosis. Chapter 2 examines dissociation …
-
Investigating the nuclear import protein KPn߀1 as a cancer therapeutic target
… others reported the elevated expression of the nuclear import protein Karyopherin Beta 1 (Kpnβ1) in various cancers. The inhibition of Kpnβ1 by siRNA silencing inhibited the proliferation of cancer cells and induced cell death via apoptosis while having little effect on non-cancer cells. These …
-
Licensing Of Cell Fate Reprogramming Via Leafy Transcription Factor Pioneering Activity
… might be a regulatory mechanism to prevent nuclear entry of LFY when not needed and that proteasome mediated degradation might be involved when an exuberated amount is present.
-
Ehrlichia chaffeensis TRP32 is a Nucleomodulin that Regulates Host Gene Expression and Post-Translational Modifications Determine its Localization and Function
… intracellular bacterium that reprograms the mononuclear phagocyte through diverse effector-host interactions to modulate numerous host cell processes, including transcription. Previously, we reported that E. chaffeensis TRP32, a type 1 secreted effector, interacts with multiple host …
-
SMAD2/3 - FOXH1 regulation during cell cycle progression upon differentiation
… activity, which results in blocking SMAD2/3 nuclear entry or by directly binding to developmental genes and co-recruiting epigenetic modifiers. Despite the importance of these interplay, the molecular mechanisms orchestrating transcriptional networks during progression of the cell cycle upon …
-
Explorer l'impact de l'élimination de la charge nette cationique des signaux de localisation nucléaire (NLS) sur le ciblage par l'importine-α
… weight molecules to freely diffuse through nuclear pores, making it challenging to target. Nuclear transport is a vital component of normal functioning in eukaryotic cells. Specialized transport proteins called importins recognize nuclear localization signals (NLSs) on proteins in the …
-
Proteomics and dynamics of the human nucleolus
… within the nucleolus immediately after the nuclear entry. The NHPX progression is dependent on pre-mRNA transcription and may link multiple RNA metabolic pathways that occur in distinct subnuclear domains.
-
The Regulation of Cellular Localization of Both Active and Inactive ERK1/2
… into the spatial control of ERK1/2 I examined nuclear import of ERK2 mutants, the interactions of ERK2 with karyopherins β1 and β2, and explored the roles of cytoskeletal elements, motor proteins and scaffolding complexes in the localization of ERK1/2. Mutation or dysregulation of the ERK1/2 …
-
Genetic Studies on the Circadian Clock in the Model Organism Drosophila Melanogaster
… gain of shaggy function concurs with an advanced nuclear entry of the PERIOD/TIMELESS heterodimer. A model is proposed, whereby SHAGGY determines the rate of PERIOD/TIMELESS nuclear translocation through its effect on the phosphorylation pattern of TIMELESS. This screen also resulted in the …
-
Design and evaluation of drug loaded microbubbles for ultrasound guided cancer therapy
Cancer is a genetic disease, caused by mutations in the genome of normal cells. Chemical and physical damage to the cellular genome can induce these mutations resulting in the transformation of a healthy cell into a tumor cell. Over the past years, researchers have acquired a basic understanding of …