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Showing 1 to 20 of 86 for “"Nuclear Pore"”.
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Structure and Organization of Nuclear Pore Proteins
<p>The nuclear pore complex (NPC) is a large macromolecular assembly that controls the flow of molecules between the nucleus and the cytoplasm. Elucidating the structure and organization of this complex will be crucial to understanding the mechanism of nucleocytoplasmic transport, but due to its …
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The Nuclear Pore Complex: Novel Glycoprotein Constituents
<p>Nuclear pore complexes are large, uniform organelles with a mass estimated to be on the order of 10<sup>8</sup> daltons. They are ubiquitous components of the nuclear envelope, providing a channel through which macromolecular transport between the nucleus and cytoplasm occurs. Thus far, almost …
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Plasticity of the Nuclear Pore Complex Revealed With Proteomics
… and have successfully applied it to the yeast nuclear pore complex (NPC), one of the largest and most elaborate protein machines in the cell. NPC is a massive protein assembly situated in the nuclear envelope and mediating macromolecular transport between the nucleus and cytoplasm. To study the …
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Architecture of a Coat for the Nuclear Pore Membrane
<p>Nuclear pore complexes (NPCs) reside in the nuclear membrane and mediate macromolecules exchange between the nucleus and cytoplasm. Although the protein components of NPCs, termed collectively nucleoporin, have been identified, how nucleoporins arrange in NPC, however, is still an enigma. NPC is …
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Discrete 3D Model of Molecular Diffusion Through the Nuclear Pore Complex
Nuclear pore complexes (NPCs) are passageways that exist within the nuclear envelope (NE) of a eukaryotic cell. Molecular cargo travel through the passageways to either import to the nucleus or export to the cytoplasm of the cell. Efficient export of certain cargo is necessary for maintained health …
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Biophysical Characterization of Structure and Dynamics of Nuclear Pore Complex Components
<p>The Nuclear Pore Complex (NPC) mediates nucleo-cytoplasmic transport in all eukaryotes and is among the largest cellular assemblies of proteins, called nucleoporins (nups). The details of NPC architecture, dynamics, and mechanism are still unknown. NPCs can be dissected biochemically into …
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Structural and biochemical characterization of nuclear pore complex structural scaffold sub-complexes
The nuclear pore complex (NPC) is a large, modular protein assembly that regulates nucleocytoplasmic transport in all eukaryotes. The ~60-120 MDa NPC is a modular assembly of multiple copies of ~30 distinct proteins that are arranged into biochemically distinct sub-complexes. We believe that the …
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Investigation of Nuclear Pore Complex Protein Interactions and the Implications for Nuclear Transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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Investigation of nuclear pore complex protein Interactions and the implications for nuclear transport
… exchanging proteins and RNA, for a variety of nuclear and cytoplasmic processes which act in concert. The nuclear pore complex is responsible for controlling the transport of large molecules into and out of the cell nucleus. It is perhaps the largest protein structure in eukaryotic cells, and …
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Synthetic ground truth of biological shapes — Simulating variable Nuclear Pore Complexes for Microscopy
The Nuclear Pore Complex (NPC) is the only passageway for macromolecules between the nucleus and cytoplasm and an important reference standard in microscopy: it is intrinsic to cells, has a high copy number, it is massive and stereotypically arranged. The average architecture of NPC proteins has …
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Proteomic, Bioinformatic and Functional Characterization of the Nuclear Pore Complex of the African Trypanosome
… the boundary of a double membrane, known as the nuclear envelope (NE). Transport across the NE is mediated by large protein assemblages known as nuclear pore complexes (NPCs). Yeast and vertebrate NPCs are comprised of about 30 proteins, termed nucleoporins (Nups), which are present in multiple …
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Structure and Dynamic Studies of the Nuclear Pore Complex at the Single-Molecule Level
Nuclear pore complexes (NPCs) are large macromolecular structures forming the only known direct route across the double bilayer membrane of the nuclear envelope. The NPC structure has been extensively explored in an effort to elucidate the mechanisms by which they control transport. Many of these …
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Deep Learning Approach for Cell Nuclear Pore Detection and Quantification over High Resolution 3D Data
… task of segmenting and quantifying cell nuclear pores in high-resolution 3D microscopy data is critical for cellular biology and disease research. This thesis introduces a deep learning pipeline crafted to automate the segmentation and quantification of nuclear pores from high-resolution …
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Core Components Of The Nuclear Pore Bind Unique States Of Chromatin And Mediate Polycomb Repression
While the Nuclear Pore Complex’s (NPC) canonical function is to act as a selective barrier across the Nuclear Envelope (NE) and regulate nucleocytoplasmic traffic, recent studies have shown that Nucleoporins (Nups), the 30 proteins that comprise the NPC, are also involved in chromatin-related …
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Structural elucidation of a common architecture of the nuclear pore complex and COPIl vesicle coats
Nuclear pore complexes (NPCs) are massive protein assemblies that perforate the nuclear envelope and form the exclusive passageway for nucleocytoplasmic transport. NPCs play critical roles in molecular transport and a myriad of other cellular processes. Elucidation of the structure of the NPC is …
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Biophysical and structural characterization of components from the nuclear pore complex and the ubiquitin pathway
… of biological evolution. One such system is the nuclear envelope (NE), composed of an inner and outer membrane, used to form a nucleus and enclose the cell's genome. Access to the nucleus from the cytoplasm is mediated by a massive macromolecular machine called the nuclear pore complex (NPC). The …
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Conformational Dynamics of the Nuclear Pore Complex Studied With Coarse-Grained Modelling and Polarized Light Microscopy
… I explored the organization and dynamics of the nuclear pore complex (NPC) through coarse-grained modelling and polarized-total internal reflection fluorescence (pol-TIRF) microscopy. The NPC is a large (~120 MDa in humans) macromolecular assembly that controls the flow of molecules into and out …
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Studies on the interaction of HSV-1 multifunctional protein ICP27 with the nuclear pore complex proteins
… levels. ICP27 contains both a nuclear localization signal and a nuclear export signal and shuttles between the nucleus and the cytoplasm. It interacts directly with various cellular proteins including REF and cellular mRNA export receptor TAP to export viral mRNAs. However other …
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