Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Nuclear Lamina"”.
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Changes in the Oligodendrocyte Progenitor Cell Nuclear Lamina During Ageing
… thesis was to explore a possible role of the nuclear lamina (a nucleoplasmic protein scaffold) in the functional response of OPCs to the mechanical properties of their microenvironment. Work in this thesis identified changes in the expression levels of the nuclear lamina proteins Lamin A/C and …
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Lamin A and lamin C are differentially dysfunctional in autosomal dominant Emery-Dreifuss muscular dystrophy
… lamins, together with B-type lamins, form the nuclear lamina, a network of intermediate filament proteins underlining the nuclear envelope. In order to ascertain the role lamin A and C separately contribute to the molecular phenotype, we analysed ten LMNA mutations and one single nucleotide …
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"""TSA-Seq"": a novel proximity mapping approach for studying three dimensional genome organization and function"
… and distribution in the three dimensional (3D) nuclear space, and the inability to connect three dimensional microscopic information with the linear genome sequence. In this dissertation, I describe ""TSA-Seq"", a novel proximity mapping approach I have developed to study 3D genome organization. …
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UNDERSTANDING LAMIN A/C AND ITS ROLES IN DISEASE PATHOLOGIES
THE NUCLEAR LAMINA, COMPRISED OF THE A AND B-TYPE LAMINS, REGULATES CHROMATIN ORGANIZATION AND GENE TRANSCRIPTION. MOUSE MODELS WERE DERIVED TO STUDY THE ROLE OF THE A-TYPE LAMINS IN INTESTINES AND SKIN HOMEOSTASIS AND ONCOGENESIS. MICE LACKING LMNA IN THEIR GASTROINTESTINAL (GI) EPITHELIUM HAVE NO …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
… single-molecule super-resolution imaging of nuclear lamina proteins lamin B1, lamin A/C, and emerin, and the microtubule protein alpha-tubulin. The single-objective dithered light sheet achieves a localization precision below ten nanometers in xy and below 12 nanometers in z, enabling a range …
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Genomic and microscopic dissection of nuclear compartmentalization and function
… for gene expression regulation. Abnormal nuclear organization is related to disease. Microscopy measurements of individual genes reveal a population-based, statistical gradient of increased gene activity from the nuclear periphery to the center. Sequencing-based methods (Hi-C, DamID) …
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CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY
… mechanical signals (EMS) can regulate nuclear morphology and chromatin dynamics via the physical link from focal adhesions to chromatin via the cytoskeleton, linker proteins on the nuclear envelope and the nuclear lamina. However, how such force transmission from cytoskeleton to the …
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Spatially-resolved transcriptomic mapping in live cells using peroxidase-mediated proximity biotinylation
… RNAs localized to mitochondria-ER junctions and nuclear lamina, two compartments that are recalcitrant to classical biochemical purification. Similarly, APEX-Seq can isolate RNAs from mitochondrial matrix, ER-associated RNAs, OMM-associated RNAs, and potentially other non-membrane bound …
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THE IMPACT OF REPLICATION STRESS ON THE EPIGENOME OF MOUSE EMBRYONIC STEM CELLS
… from inactive to active states and from lamina-associated to H3K27me3-related inactive compartments, consistent with H3K27me3 rearrangements and a weaker nuclear lamina. These observations suggest that active H3K27 methylation may occur in gene deserts near stalled replication forks or …
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Identificazione delle Lamine di tipo A come nuovi substrati della protein chinasi Akt/PKB
… An interesting mechanism that mediates nuclear translocation of Akt has been described in human mature T-cell leukemia: the product of TCL1 gene, Tcl1, interacts with the PH domain of phosphorylated Akt, thus driving Akt to the nucleus. In this context, Tcl1 may act as a direct …
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Mechanotransduction at the nuclear envelope: the role of forces in facilitating embryonic stem cell fate decisions
… structural and chromatin binding proteins in the nuclear envelope, notably, the lamins (LMNA), Lamin B receptor (LBR) and components of the LINC complex (nesprins/KASH). The structural proteins in the nuclear envelope regulate both the mechanical response of the nucleus to force and the …
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Dynamic organization of chromosomes in the mammalian cell nucleus
Uncovering the motifs of a higher order nuclear architecture and its implications on nuclear function has raised increasing interest in the past decade. The nucleus of higher eukaryotes is considered to display a highly dynamic interaction of DNA and protein factors. There is an emerging view that …
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Prelamin A Influences a Program of Gene Expression In Regulation of Cell Cycle Control
… that constitute a major part of the eukaryotic nuclear lamina—a tough, polymerized, mesh lining of the inner nuclear membrane, providing shape and structural integrity to the nucleus. Lamin A (LA) filaments also permeate the nucleoplasm, providing additional structural support, but also …
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Recognizing epigenetic patterns that mark the control of cell state in differentiation and disease
… as super-enhancers, distal-enhancer-looping, nuclear lamina, and master transcription factors. Ensembles are also disproportionately affected in both HD and Alzheimer's disease. Together, this thesis presents a toolkit for recognizing and understanding epigenetic patterns that can further …
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The role of death-associated protein kinase in endothelial apoptosis under fluid shear stress
… endothelial mechanotransduction and the role of nuclear lamina is further evaluated.^ Using bovine aortic endothelial cells, we have shown that laminar shear stress modulates DAPK expression. Initially, our study examined the time-dependent effects of conditioning cells with shear stress on …
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Computational approaches for analyzing regulatory regions in the human genome
… cytological distance of chromatin to a specific nuclear structure. Norma is an all-in-one software package for TSA-seq data processing, which covers from the mapping of the raw NGS reads, to the calculations of the enrichment score and conducting all kinds of the integrated analysis of enrichment …
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Novel Intermediate Filaments in the Nuclear Envelope: Molecular Cloning and Analysis of Lamins A and C
… A, B and C are the major polypeptides of the nuclear lamina, the filamentous meshwork intercalated between chromatin and the inner nuclear membrane. Amino acid sequences have been deduced from cDNA clones of human lamins A and C which show identity with the exception of an additional 98 amino …
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Multiscale Modeling of Genome Organization: Bridging Polymer Physics, Molecular Dynamics, and AI
… apply computational frameworks to investigate nuclear genome organization at high spatial and temporal resolution. Our approaches integrate biophysical modeling and generative artificial intelligence to address complementary aspects of nuclear architecture. In Chapter 1, we provide an overview …
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Characterisation of the multi-isomeric protein nesprin-1 in p-body and mRNA dynamics
Nuclear envelope spectrin repeat proteins, or Nesprins, are a novel family of nuclear and cytoskeletal proteins with rapidly expanding roles as intracellular scaffolds and linkers. Nesprins are characterized by a central spectrin repeat (SR) rod domain and a C-terminal KASH domain, which acts as a …
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Integrative analysis of transcriptional activity and genome architecture changes upon viral infections
To study the interplay between spatial nuclear architecture and transcriptional activity during viral infections, I employed a genome-wide chromosome conformation capture approach (Hi-C) on infected murine and human cells and further enriched those libraries for genomic loci of interest and the …
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