Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"NuRD"”.
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Colocalization between Akirin, the NuRD Complex, and Histone Modifications
… as the nucleosome remodeling and deacetylase (NuRD) complex. <em>Drosophila</em> polytene chromosomes were used to visually identify colocalization between Akirin and subunits of the NuRD complex. The NuRD subunit, HDAC1, functions to remove acetyl groups from histone tails. Therefore, the …
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Dissecting the role of CHD4 in the NuRD and ChAHP complexes
… in gene regulation as a core component of the NuRD complex, CHD4 is also found within the ChAHP complex, which contributes to silencing of transposable elements (TEs). CHD4 generally functions to increase nucleosome density, however the specific chromatin remodelling mechanisms employed by NuRD …
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The NuRD complex regulates chromatin dynamics driving exit from naïve pluripotency
The Nucleosome Remodelling and Deacetylase (NuRD) complex is essential for exit from naive pluripotency both in mammalian embryonic stem cell (ESC) culture and in vivo. However, the specific mechanisms of chromatin modulation by NuRD in this process have not been fully characterised. This study …
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The Role of the Nucleosome Remodeling and Histone Deacetylase (NuRD) Complex in Fetal γ-Globin Expression
… Chromatin modifying complexes, including MBD2-NuRD and GATA-1/FOG-1/NuRD play a role in γ-globin gene silencing, and Mi2β (CHD4) is a critical component of NuRD complexes. In the studies presented in Chapter 2, we observed that the absence of MBD2 in a sickle cell mouse model leads to a …
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The Nucleosome Remodelling & Deacetylase complex: Genome folding & transcriptional regulation
… The Nucleosome Remodeling and Deacetylase (NuRD) complex is one of four major ATP-dependent chromatin remodeling complexes and has been shown to be crucial for early embryonic development, to maintain transcriptional heterogeneity and to modulate the physical dynamics of regulatory genomic …
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The synthetic multivulva genes and their suppressors regulate opposing cell fates through chromatin remodeling
… of a Nucleosome Remodeling and Deacetylase (NuRD)-like complex and heterochromatin protein 1 (HP1). In addition to a NuRD-like complex, which deacetylates lysine nine of histone H3 (H3K9), and HP1, we found two histone methyltransferase (HMT) genes (met-1 and met-2) that act as class B synMuv …
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Investigation into the Akirin Subcellular Localization and Developmental Interactions
… and the Nucleosome Remodeling Deacetylase (NuRD) complex. Through transfection of <em>Drosophila</em> S2 cells with Akirin mutant constructs we were able to visualize the localization patterns of Akirin and how the patterns relate to the Nuclear Localization Signal (NLS). Data shows that …
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Uncovering The Zeb1 Interactome to Identify Novel Regulators of Metastatic Non-Small Cell Lung Cancer
… nucleosome and deacetylase remodeling complex (NuRD). Through treatment with class I HDAC inhibitors Trichostatin A, we identified that ZEB1 homodimerizes and determine that acetylation at lysine residue 811 regulates this association. Furthermore, we identify the NuRD complex as a novel ZEB1 …
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Investigating the molecular function of Akirin during skeletal myogenesis in Drosophila melanogaster
… a novel interaction between Akirin and the NuRD chromatin remodeling complex. These results also show context-dependent roles of Akirin at the transcriptional level, as interactions with SWI/SNF family function to facilitate gene expression, and interactions with NuRD family may function …
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CHD Chromatin Remodelers Promote Epigenetic Control of Development in Arabidopsis thaliana
… not function as a member of a multi-subunit Mi-2 NuRD complex. Instead, PKL acts as a monomer in vivo and promotes the epigenetic modification H3K27me3, which is associated with transcriptional repression of tissue-specific genes by the PRC2. Thus, PKL plays an important role in promoting tissue …
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Discovery of Novel Ubiquitin- and Methylation-Dependent Interactions Using Protein Domain Microarrays
… in their unmethylated form, interact with NuRD complex components. These findings provide in vitro evidence that methylation of H3TMs can promote novel interactions with PHD finger- and Tudor domain-containing proteins and block interactions with the NuRD complex. We propose that these …
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The TAZ Protein Interactome in Striated Muscle
… Epigenetic regulators were also represented: NuRD complex, FACT complex, and SWI/SNF complex. We focused on characterizing the TAZ interaction with the Wnt co-repressor TLE3 in myogenic cells. In myogenic cells, TAZ and TLE3 interact and co-localize within the nucleus. Functionally, TAZ and …
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Regulation and Function of Zeb1 Acetylation In Lung Adenocarcinoma Progression and Metastasis
… the nucleosome remodeling and deacetylase (NuRD) complex to bind the promoter of <em>mir200c-141</em> and <em>SEMA3F </em>genes. RNA-sequencing revealed that WT ZEB1 and K811Q ZEB1 downregulate the expression of epithelial genes to enhance NSCLC motility, invasion, and metastasis, while the …
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Regulation of mammalian SINE transcription
… of two corepressor complexes, SIN3 and NuRD. Whether MBD proteins repress SINE transcription in a DNA methylation-dependent manner was further investigated using systems with low or near absent DNA methylation and, in the case of MeCP2 protein, by its direct removal. MeCP2 was found to …
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Characterising the interaction between TRIM28 and members of the Krab-ZFP family in transcription silencing
… and the nucleosome remodelling and deacetylase (NuRD) complex. Although many protein partners involved in silencing have been identified, the molecular basis of the protein interactions that mediate silencing remains largely unclear. TRIM28 belongs to the tripartite motif (TRIM) protein family …
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REGULATION OF CANCER METASTASIS BY PROTEIN KINASE D1: A GLOBAL REGULATORY CASCADE
… remodeling and histone deacetylase protein (NuRD) which is overexpressed in all the cancers. MTA1 is an initiator of epithelial and mesenchymal transition and is responsible for cancer cells metastasizing to different organs of the body. Expression of MTA1 directly correlates with the …
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THE PHD FINGER OF SP140: A STRUCTURAL AND FUNCTIONAL STUDY
… and the CHD3/Mi2 component of the NuRD complex through recognition of the SUMO moieties by the SIMs of these proteins (Ivanov et al, 2007). Through NMR and ITC titrations we found that Sp140 PB tandem is able to bind to a 15mer H3K9ac synthetic peptide. We excluded any PHD finger …