Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 23 for “"Notochord"”.
-
Mechanisms of Copper-Dependent Notochord Formation in Zebrafish
… zebrafish, which includes a strikingly distorted notochord. The studies described here establish the specific genetic etiology of this distortion and reveal a number of gene-gene and gene-nutrient interactions critical to notochord morphogenesis. We first demonstrate that the notochord distortion …
-
Notochord morphogenesis as a mechanical driver of embryo axis elongation
… axis is currently not well understood. The notochord, a rod-shaped tissue running through the middle of all vertebrate embryos, is a key candidate for driving this process. Cells in the notochord undergo an expansion that is constrained by a stiff sheath of extracellular matrix that …
-
Investigating non-canonical vertebral development in the zebrafish model system
… initially form as mineralised rings within the notochord sheath (chordacentra) and then secondarily, bone is deposited around this (perichordal centra and arches). Notochord cells (chordoblasts) have been implicated in chordacentrum mineralisation and patterning in zebrafish and Atlantic salmon, …
-
Analysis of the Zebrafish Segmentation Clock
… symmetric blocks of tissue flanking the notochord called somites. Somites are generated sequentially and periodically from an unsegmented tissue called the presomitic mesoderm (PSM) by a process called somitogenesis. <br></br><br></br> Underlying the periodicity of somitogenesis are …
-
How to build a chordate: Multiscale decomposition of axial morphogenesis in the amphioxus, Branchiostoma lanceolatum
… The epicentre of the body plan is the notochord, located at the axial midline, which is flanked dorsally by a neural tube, bilaterally by a metameric pattern of somites, and ventrally by a primitive gut tube. If we are to understand how chordates emerged in evolution, and have …
-
Investigating the Biological Mechanisms Underlying Axis Morphogenesis Using a Novel Zebrafish Model of Idiopathic-like Scoliosis
… implicated dysregulation of the ECM and notochord morphogenesis in CTD pathogenesis and showed that overexpression of ECM genes in the notochord can modulate the zebrafish embryonic axis. Overall, my work furthers our understanding of the biological mechanisms perturbed in AIS and may one …
-
Establishing Image-Based Profiling Methods for Single-Cell Transcriptomics and Morphometrics in the Cephalochordate Amphioxus
… of morphogenesis in the developing amphioxus notochord, recapitulating previous single-cell morphometric (scMorph) analyses as a proof of principle and then dissecting the changes to notochord structure resulting from pharmacological perturbation. Taken together, this thesis builds a …
-
The mechanical characteristics and differentiation potential of the axial progenitor region in vertebrate embryos
… atomic force microscopy (AFM). I showed that the notochord, the NMCs, and the PSM are distinct mechanically; with the notochord being the stiffest and the PSM the softest. In addition, I observe an anterior-to-posterior reducing stiffness gradient within the NMC region. These findings are …
-
Evaluation of the biological feasibility of white stumpnose, Rhabdosargus globiceps, as a potential aquaculture candidate in South Africa
… of feeding, swimbladder inflation and notochord flexion than at 15°C. The results suggest that the highest quality larvae are achieved at a rearing temperature of 20°C. This thesis describes the induction of spawning and larval rearing of Rhabdosargus globiceps. The ontogeny of the …
-
Sonic hedgehog induces Motor Neuron Differentiation in P19 Embryonic Carcinoma Cells
… and secreted from two signalling centers, the notochord and the floor plate, where it functions as a morphogen to induce early dorso-ventral patterning of the cenral nervous system (CNS). More recently, multiple actions of Shh during CNS development have been discovered in additional sites, …
-
The role of N-cadherin and S1P/S1P1 in pancreas development
… of pancreatic epithelial cells. For instance, notochord, endothelial cells, and pancreatic mesenchyme are essential for the development of the pancreas. N-cadherin deficient-embryos suffer from dorsal pancreas agenesis due to apoptosis of the mesenchyme surrounding the pancreatic epithelium. …
-
The Role of GDF-3 in Patterning the Early Embryo
… mutants, disorganized, distal migration of the notochord results in dorso-ventral re-arrangment of the entire embryo. This finding provides a genetic basis for dissecting the tightly coupled processes of morphogenesis and cell fate determination in the mammalian embryo.</p>
-
An Investigation Into The Mineral And Mechanical Properties Of The Canine Intervertebral Disc And Its Clinical Implications
… of collagen fibres, loss of proteoglycans and notochord cells and a reduction in water content; however, how these processes are linked to future disc herniation remains unknown. Most studies to date have focused on imaging changes and histopathology of affected discs, but here we take a novel …
-
Characterization of Dishevelled Associated Activator of Morphogenesis 2 (Daam2) in Wnt Signaling During Early Embryonic Development
… dynamic actin reorganization such as mesoderm, notochord and neural tube. The knockdown of Daam2 in Xenopus embryos specifically produces neural tube closure defect suggesting a role in non-canonical signaling. Indeed studies examining the role of Daam2 in canonical Wnt signaling found no role …
-
CAMK-II: AN INTEGRAL PROTEIN IN CELL MIGRATION
… segmentation resulting in undulation of the notochord. While it is clear ncWnts are necessary for C&E, many of the downstream effectors regulating these cell movements have not been defined. Previous research has shown that activation of ncWnt signaling through Wnt5 or Wnt11 results in an …
-
Συμβολή εις την μελέτην των χορδωμάτων
… Study deals with the development and fate of the notochord and the pathology, localization, clinical course and clinical features of chordomas. The second part is occupied by the study of the seventeen cases (17) of chordomas. The patients had been hospitalized in a period from 1962 till 1981. The …
-
The roles of T and Tbx6 during gastrulation and determination of left/right asymmetry
… after the seventh pair, have no distinguishable notochord, have a convoluted neural tube and die at E.10.5. In this study we investigate the phenotype of a dominant negative allele of T, TWis. Like homozygous embryos carrying the null allele of T, TWis homozygous mutants have left/right asymmetry …
-
The Role of Notch Signalling in Amplifying the Cellular Response to Sonic Hedgehog during Dorsal-Ventral Patterning of the Spinal Cord
… of the embryo, namely the floor plate and the notochord. In recent years, a lot of research has been carried out to understand how cells interpret the Shh signal and translate this information in order to adopt a specific neuronal fate.<br/><br/>The Notch pathway plays a crucial role during …
-
Left, Right, and Center: A Novel Midline Barrier Separates Asymmetric Extracellular Matrix During Intestinal Development
… transition of the endoderm, and the notochord is not sufficient for its synthesis. Although the function of the midline could not be evaluated with any of the attempted ablation efforts, we predict that the DM midline barrier forms a functional boundary between the left and right …
Page 1 of 2