Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"Notch signalling pathway"”.
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Mechanistic Insights into the Notch signalling pathway through Molecular Condensation
… identity, and cell positional information. A key signalling pathway in interpreting this information is the Notch signalling pathway. It is currently poorly understood how Notch is able to play a titratable role in signal transduction as our current model does not effectively translate how …
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A novel genome-wide approach to identify in vivo targets of Notch signalling
The Notch signalling pathway regulates many developmental processes in metazoan embryos and adults such as cell proliferation, stem cell maintenance, cell fate specification and apoptosis. Despite the importance of this pathway, few targets have been identified, with the Hes (Hairy and …
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Super-resolution Microscopy: Novel Developments and Optimisations
… of a DNA-binding transcription factor for the Notch signalling pathway, deep within the whole salivary glands of Drosophila. Characterisation and optimisation of the microscope and the subsequent image analysis pipeline, to extract dynamics of single molecules at such depths is also discussed. …
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FKBP14 in Notch Signalling and Drosophila Development
… growing list of transmembrane proteins including Notch and Amyloid Precursor Protein. Autosomal dominant mutations in the genes encoding human PS have been closely linked to cases of early onset familial Alzheimer's Disease (FAD), and these FAD-linked PS mutations influence the activity of …
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DLL4 and JAG1 in the Glioblastoma Response to Temozolomide Chemotherapy
… promising target for glioblastoma therapy is the Notch signalling pathway, which can be suppressed using gamma-secretase inhibitors such as dibenzazepine (DBZ). We investigated the effects of the Notch ligands, Delta-like 4 (DLL4) and Jagged-1 (JAG1) on tumour resistance to TMZ and GCSCs. Methods: …
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Regulation of Notch Ligands Dll1 and Jag1 by Pax6 during cortical development
… by Pax6 in the developing cortex. Members of the Notch signalling pathway were potential Pax6 targets of particular interest since Notch signalling plays a crucial role in the maintenance of neural progenitor cells during development and consequently plays a critical role during corticogenesis. …
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An investigation into the mechanisms of angiogenesis and breast cancer metastasis
… in HUVECs resulted in increased upregulation of Notch signalling pathway (major regulator of angiogenesis) ligand DLL1 and DLL4. Additionally, we investigated genomic changes that may be involved in the development and progression of breast to brain metastasis (BBM). Whole exome sequencing (WES) …
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Notch signalling in spinal cord patterning: Crosstalk and fate decisions
… with particular focus on the highly conserved Notch signalling pathway. The major, and most well studied, role of Notch signalling during neural development is progenitor maintenance. This thesis explores the additional roles of Notch signalling in signalling crosstalk and cell fate decisions. …
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Transcription and chromatin dynamics in the Notch signalling response
… in different cell types, often directed by cell signalling pathways and the pre-existing chromatin environment. The highly-conserved Notch signalling pathway is involved in many cell fate decisions during development, activating different target genes in different contexts. Upon ligand binding, …
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Immune Mechanisms of Mesenchymal Stem Cell Therapy for Acute Graft versus Host Disease
… cell contact. Blocking studies revealed that the Notch signalling pathway was essential for this process. A humanised mouse model of aGvHD based on delivery of human peripheral blood mononuclear cells (PBMC) to NOD-scid IL-2rγnull (NSG) mice was developed to allow robust exploration of the role of …
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Investigating the role of Notch signalling during mesoderm development and cell proliferation
… developmental and diseases processes. The Notch signalling pathway is a cell-contact-dependent pathway known to regulate cell proliferation, differentiation, and specification in a context-dependent manner. Owing to its crucial function during all stages of life, it is highly conserved …
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Single Molecule Localisation with Fourier Light Field Microscopy
… the dynamics of a transcription factor for the Notch signalling pathway inside the whole salivary glands of Drosophila in 3D for the first time. Ultimately, this thesis aims to demonstrate the versatility of this tool and the potential it offers for gaining new insights into nano-scale …
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Notch activation and clone fate in intestinal homeostasis and inflammation
The Notch signalling pathway is a highly conserved pathway that has important and often differing roles and functions depending on the tissue. In the intestinal epithelium Notch signals are important for determining cell fate between the absorptive and secretory lineage and regulating the …
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Probing the druggability of the Notch1 ankyrin domain using a fragment-based approach
Notch signalling is a highly conserved pathway that is important in the developmental processes that control cell differentiation and cell fates. This canonical pathway involves binding of a transmembrane ligand in one cell to the extraceullular domain of a transmembrane Notch receptor in an …
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The Role of Notch Signalling in Amplifying the Cellular Response to Sonic Hedgehog during Dorsal-Ventral Patterning of the Spinal Cord
… to adopt a specific neuronal fate.<br/><br/>The Notch pathway plays a crucial role during neurogenesis of both invertebrate and vertebrate embryos. It has also been demonstrated that the Notch and Shh signalling pathways can interplay in various developmental contexts. In this project, I have …
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Interpreting Notch signals: characterisation of dynamic transcription hubs by live imaging
The Notch signalling pathway, crucial for development, activates upon cell-to-cell contact. Despite lacking any amplification, activation of target genes is achieved robustly and efficiently after contact-induced cleavage of the Notch transmembrane protein. How relatively few molecules of the …
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Identifying Novel Molecular Mechanisms Underlying Lateral Line Organ Development in Sterlet
… closely related cell types. To gain insight into signalling pathways potentially involved in AO and/or neuromast development, I cloned cDNA fragments of 50 signalling pathway-related genes from the paddlefish gene-set and 26 other signalling pathway-related genes selected via a candidate approach, …