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Showing 1 to 15 of 15 for “"Nostoc commune"”.
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Molecular Biology of Desiccation Tolerance in the Cyanobacterium Nostoc commune
… in the cyanobacteria with emphasis on Nostoc commune. Analysis of DNA from 41 samples of desiccated Nostoc spp. of varied age and global distribution led to the amplification of 43 independent tRNALEU(UAA) group 1 intron sequences. Phylogenetic analysis of the entire data set made it …
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Organization of nifH and nifD genes in Nostoc commune UTEX 584
Nostoc commune UTEX 584 is a photosynthetic desiccation-tolerant cyanobacterium capable of fixing nitrogen. Biotinylated nifH and nifD gene probes from Azotobacter vinelandii (a gift from Dr. Dennis Dean) hybridized to nifH and nifD sequences isolated from Nostoc commune UTEX 584. This result …
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Morphological, biochemical and molecular characterization of desiccation-tolerance in cyanobacterium Nostoc commune var. Vauch
… of the desiccation-tolerant cyanobacterium Nostoc commune are embedded within, and distributed throughout, a dense glycan sheath. Analysis of the glycan of field materials and of pure cultures of N. commune DRH1 through light and electron microscopy, immunogold-labelling and staining with …
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Characterization of the nifUHD cluster and a new myoglobin-like gene from Nostoc commune UTEX 584
… genomic DNA fragment previously isolated from <i>Nostoc commune</i> UTEX 584 (Defrancesco and Potts 1988), determined the exact locations of the <i>nifU, nifH</i>, and <i>nifD</i> genes and identified two potential stem loop structures, a direct repeat, and an ORF that codes for a protein with a …
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Partial structural characterization of the cytoplasmic hemoglobin of Nostoc commune UTEX 584 expressed in Escherichia coli
… the nitrogen fixing apparatus in cyanobacterium Nostoc commune revealed a gene encoding for a hemoprotein, known as cyanoglobin. The cyanoglobin gene was isolated and subcloned into Escherichia coli previously. The study presented here encompasses the optimization of growth conditions for the …
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Structural and Functional Characterization of Cyanoglobin: A Peripheral Membrane Hemoglobin in Nostoc commune UTEX 584 (Cyanobacteria)
… fixing (nif) genes in the cyanobacterium Nostoc commune UTEX 584 revealed a gene encoding a hemoprotein, named cyanoglobin. The cyanoglobin gene was isolated and subcloned into Escherichia coli previously. Cyanoglobin possesses a high oxygen affinity. The study presented here investigated …
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Molecular analysis of genes involved in carbohydrate metabolism in the desiccation-tolerant cyanobacterium Nostoc commune UTEX 584
… of the desiccation-tolerant cyanobacterium <i>Nostoc commune</i> UTEX 584 (<i>Nostoc</i> 584). The Wsp polypeptides synthesized and secreted by field material of N. <i>commune</i> have been extensively characterized and shown to exist as three isoforms with molecular masses of 33, 37 and 39 …
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Characterization of IphP from Nostoc commune UTEX 584 and a Dual Specificity Protein Phosphatase from Anabaena PCC 7120
… protein phosphatase from the cyanobacterium Nostoc commune UTEX 584 bearing the active-site signature sequence of eukaryotic tyrosine-specific and dual-specificity protein phosphatases. The catalytic properties and substrate specificity of IphP were examined in detail. The enzyme was able to …
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Isolation and partial characterization of a water stress protein of the desiccation-tolerant cyanobacterium Nostoc commune UTEX 584 expressed in Escherichia coli
A desiccation-tolerant cyanobacterium <i>Nostoc commune</i> accumulates a novel group of water stress proteins (Wsp) in response to cycles of repeated drying and rehydration. Antibodies, specific for Wsp, were used to screen a lambdafix II library of <i>N. commune</i> UTEX 584 Bam H1 DNA fragments …
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Carbohydrate-Interacting Proteins from Two Nostoc (Cyanobacteria) Species
Cyanobacteria of the Nostoc genus are known for the thick, mucilaginous carbohydrate coatings that they produce. In this work, two examples of cyanobacterial glycobiology are considered, each of which involves a cyanobacterium of the Nostoc genus. The first portion of this work details attempts to …
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The Effect of Nitrates, pH, and Dissolved Inorganic Carbon Concentrations on the Extracellular Polysaccharide of Three Strains of Cyanobacteria Belonging to the Family Nostocaceae
… (Anabaena PCC7120, A. variabilis and Nostoc commune), all belonging to the family Nostocaceae, were found to be capable of modulating the production and chemical composition of extracellular polysaccharides (EPS) in response to carbon and nitrogen availability as well as pH. While the …
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Water stress protein is secreted, is subject to rapid proteolysis upon rehydration of desiccated cells, and may be glycosylated
… of the desiccationtolerant cyanobacterium <i>Nostoc commune</i> (Scherer, S. and Potts, M. 1989. J. BioI. Chem. 264:12546-12553). In the original study, several inconsistencies were noted and are addressed in this body of work. Firstly, the microheterogeneity noted on two dimensional …
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Aðstoð við myndun lífrænnar jarðvegsskánar við endurheimt vistkerfa á Íslandi
… smitun með ræktuðum blágrænubakteríum (Nostoc commune), muldu efni úr náttúrulegri lífskurn og tilbúnum áburði áburði (n=5), á jarðvegi af rofnu landi undir stýrðum aðstæðum í tilraunastofu í 6 mánuði. Allar meðferðir og ómeðhöndluð viðmið þróuðu sýnileg einkenni lífskurnar, svo sem …
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Utility of Ion Mobility – Mass Spectrometry in Peptidoglycan Structural Determination
… (CCS). PG of two filamentous cyanobacteria, Nostoc commune and Limnospira platensis, were chosen for this work as they have not been characterized before now despite commonplace use as sources of nutrition. The analyses revealed that muropeptides can be organized into distinct regions of …
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Molecular analysis of cyanobacterial RNA polymerase genes
… and rpoC2 are incomplete) of the cyanobacterium Nostoc commune have been cloned, sequenced, and expressed both in vivo and in vitro using E. coli systems. The rpo genes encode large subunits of DNA-dependent RNA polymerase. Two genes in N. commune, rpoC1 and rpoC2, correspond to rpoC of E. coli, …