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Showing 1 to 20 of 149 for “"Northern blot"”.

  1. Untersuchungen zur Expression, Funktion und Regulation ausgewählter Gene der Insulinfamilie

    Shirneshan, Katayoon2012

    … Insl5 und Insl6, untersucht. Mithilfe einer Northern-Blot-Analyse mit Gesamt-RNA aus unterschiedlichen Organen der adulten Maus konnte gezeigt werden, dass das Insl5-Gen sehr stark im Rektum der Maus exprimiert wird. Die Expression während der embryonalen Entwicklung konnte mittels …

    goettingen Repository record

  2. Characterization of XVEF and XvCaM : two calcium-binding proteins isolated from the resurrection plant Xerophyta viscosa

    Conrad, NailahDepartment of Molecular and Cell Biology2005

    … transmembrane domain spanning residues 90-107. Northern blot analyses of total RNA indicated that XVEF transcript increased 48 hours after 100/-lM ABA application and was present between 12 and 48 hours in response to a low temperature stress (4°C). A second gene, XvCaM, was isolated from a low …

    cape-town Repository record

  3. Expression analysis of Drosophila melanogaster microRNAs

    Yalcin, Abdullah2012

    … der Drosophila melanogaster miRNAs mittels Northern blot Analyse unterschiedlicher Entwicklungsstadien der Fliege untersucht. Zeitabhängige Expressionsmuster von miRNAs können zur Verifizierung vorhergesagter miRNA targets und biologischer Funktionen benutzt werden. Gewebespezifische …

    goettingen Repository record

  4. Tissue Distribution of a Peptide Transporter mRNA in Sheep, Dairy Cows, Pigs, and Chickens

    Chen, HongVirginia Tech1998

    … segments and other tissues as indicated. Northern blot analysis was conducted using the radiolabeled probe. In sheep (5) and lactating Holstein cows (3), hybridization was observed with mRNA from the omasum, rumen, duodenum, jejunum, and ileum. The estimated size of mRNA was 2.8 kb. No …

    vt Repository record

  5. Expression of Vascular Endothelial Growth Factor-A and its Receptors in Uteroplacental Tissues of the Normotensive and Hypertensive Pregnant Rat

    He, HuilingVictoria University1999

    … transcriptase-polymerase chain reaction), Northern blot analysis, immunohistochemistry and in situ hybridization, to measure the expression and localization of VEGF-A, vascular endothelial growth factor receptor 1 (VEGFR-1) and vascular endothelial growth factor receptor 2 (VEGFR-2) in …

    vu-aus Repository record

  6. The structure and sequence of the single calmodulin gene of Drosophila melanogaster

    Doyle, Kevin EdwardRice University1990

    … the use of alternative polyadenylation signals. Northern blot analysis of polyadenylated transcripts isolated from 0 to 12 hour-old embryos confirmed the existence of two mRNAs of the approximate sizes 1.7 and 1.9 kilobases. Northern blot analysis of poly-A+ RNA isolated from developmentally …

    rice Repository record

  7. The role of small RNAs in caste determination and differentiation in the bumble bee, Bombus terrestris

    Collins, DavidUniversity of East Anglia2014

    … any species. In Chapter 3, using miRNA-seq and Northern blot analysis of female B. terrestris larvae, I isolated a miRNA (Bte-miR-6001) that was more highly expressed in queen- than in worker-destined larvae. This miRNA comprised an entire intron of the gene Very high density lipoprotein (Vhdl), …

    east-anglia Repository record

  8. Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family

    Manjunath, SivalingannaUniversity of Illinois at Urbana-Champaign2011

    … compared to only 81% with either GAPC1 or GAPC2. Northern blot analysis revealed a maximum transcript accumulation 24 h after anaerobiosis and anoxic specific induced expression for gpc3 and gpc4.

    uiuc Repository record

  9. The molecular epidemiology of bluetongue virus in northern Colorado

    White, David M., author, Blair, Carol D., advisor, Beaty, Barry J., advisor, Wilson, William C., committee member, Bowen, R. A., committee member, Black, William C., IV, committee member, Salman, Mowafak D., committee memberColorado State University. Libraries2001

    … mechanism of BTV. In addition, RT-PCR and Northern blot analysis of total RNA from cell-culture models of natural infection, using both mammalian cell lines and insect cells obtained from ABADRL, were performed to characterize a possible mechanism of persistence of the virus in insect …

    colostate Repository record

  10. Analysis of alkali-inducible genes of Bacillus subtilis

    Atalla, AkramUniversität Bayreuth

    … a transcriptional anti-activator in E. coli . In Northern blot analyses, it could be shown that all three genes are induced after alkali shock. The transcription start points of the kipR and yvdT genes were identified by primer extension experiments, and it appeared that the transcription is …

    bayreuth Repository record

  11. Regulation of melanogenesis in conditionally immortalised mouse melanocytes expressing a temperature-sensitive SV40 large T antigen

    Prince, SharonDivision of Cell Biology1999

    … cell lines thus generated were analysed by northern and western blots and by enzymatic assays at the permissive temperature of the oncoprotein. Three epidermal and two dermal melanocyte clones remained pigmented and expressed tyrosinase, TRP-1 and -2 genes and the proteins encoded by them. …

    cape-town Repository record

  12. Proteins and instability elements involved in phosphoenolpyruvate carboxykinase mRNA turnover

    Hajarnis, Sachin Shrikant, author, Curthoys, Norman, advisor, Bamburg, James, committee member, Mykles, Donald, committee member, Laybourn, Paul, committee member, Wilusz, Jeffrey, committee memberColorado State University. Libraries2006

    … assays, affinity pull down assays, western blot technique and MALDI-TOF/TOF analyses. The identified proteins bind to AU- and CU-rich sequences and secondary structures found within the PEPCK 3'-UTR. A tetracycline regulated promoter system was utilized in kidney proximal tubule-like cells, …

    colostate Repository record

  13. Regulation der Metalloproteinasen Stromelysin und Collagenase durch Interferon in retinalen Pigmentepithelzellen

    Meisenzahl, ChristinaUniversität Würzburg

    … erfolgen. Als Nachweismethoden dienten die Northern-blot-Analyse, wobei für die Hybridisierung Digoxigenin-markierte antisense-RNA verwendet wurde, die durch in vitro-Transkription gewonnen wurde, sowie die RT-PCR. Als Modell diente die ARPE-19-Zelle, die sich durch ihre epitheliale …

    wurz-thes Repository record

  14. Differentielle Genexpressionsanalyse aktivierter Endothelzellen

    Schmidt, Tobias2012

    … konnte für 47 Fragmente (55%) die Regulation im Northern-blot verifiziert werden, eine methodenspezifisch überdurchschnittliche Positivrate. Die bearbeiteten 41 hochregulierten Fragmente liessen sich im Sequenzdatenbankabgleich auf 35 distinkte Gene zurückführen, 16 neue und 19 bekannte Gene, …

    goettingen Repository record

  15. Regulatory Genes of the Bacteroides Conjugative Transposon, CTnDOT

    Moon, KyungUniversity of Illinois at Urbana-Champaign2015

    … tra genes, using site-directed mutagenesis and Northern blot analysis. This region turned out to contain a small non-coding regulatory RNA that has been renamed as RteR. However, further work has to be done to examine its mechanism of action.

    uiuc Repository record

  16. Systematic analysis of genes expressed in the retinal pigment epithelium (RPE) and identification of candidates for genetic susceptibility to age-related macular degeneration (AMD)

    Abdel Rahman, Faisal MirghaniUniversität Würzburg

    … clones with no significant similarity. Reverse Northern blot hybridization was performed for 318 EST clusters to identify abundantly expressed genes in the RPE and to prioritize subsequent analyses. Representative clones were spotted onto a nylon membrane and hybridized with cDNA probes of …

    wurz-thes Repository record

  17. Characterization of ABF-1 in C. elegans and regulation of cellular growth and ID3 by human ABF-1

    Round, June L.2002

    … to overexpression of ABF-1 via western and northern blot, respectively. Flow cytometry analysis and Real-time PCR revealed that ABF-1 programs a slow down in the cell cycle, however this growth arrest is not mediated by ID3.</p>

    u-pacific Repository record

  18. MOLECULAR ANALYSIS OF FLORAL PIGMENTATION IN THE ORCHID, BROMHEADIA FINLAYSONIANA

    LIEW CHYE FONG1998

    … (PCR) generated homologous probes. Southern blot analysis revealed that PAL, CHS and F3H are encoded by small multigene families while DFR is represented by a single gene in the genome of Bromheadia. Northern blot and reverse transcriptase-PCR (RT-PCR) analysis showed that PAL is …

    nus Repository record

  19. Characterization of soybean mosaic virus coat protein-mediated resistance in transgenic soybeans

    Wang, Xinyu1998

    … Polymerase chain reaction and Southern blot analyses were used to detect the transgene. Four insertion events were detected in T₀ plants by Southern blot analyses. Self-pollination of plants yielded four homozygous transgenic lines in the T₃ generation; they are transgenic lines 7b-11 …

    iastate Repository record

  20. Expression of recombinant porcine preprorelaxin in Nicotiana tabacum

    Buswell, Walter ScottVirginia Tech2006

    … plants that had demonstrated gene expression by northern blot analysis. Preprorelaxin was only identified in extracts from transgenic plants that contained the insoluble protein fraction, as determined by western blot analysis. Additionally, an increased yield of preprorelaxin was identified …

    vt Repository record

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