Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"Nonspecific binding"”.
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Evaluation of different in vitro surrogates to represent nonspecific binding for tissue: plasma water partition coefficient predictions
With the growing use of physiologically-based pharmacokinetic (PBPK) modeling to predict the pharmacokinetics of a drug, accurate prediction of the tissue: plasma water partition coefficients (Kp,m) has become increasingly important. In these predictions, drug-lipid interactions have been …
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Development of polymer-coated nanoparticle imaging agents for diagnostic applications
… surface charge and how it relates to the nonspecific binding of nanoparticles in cells, tissues and other complex biological samples was studied. We have found that highly charged (negative and positive) particles exhibit significant nonspecific binding to biomolecules and other cellular …
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Multi-channel solutions for optical labelfree detection schemes based on the interferometric and grating coupler principle
… A promising approach for applications where binding kinetics is monitored is the interferometric sensor principle. The Young-Interferometer which consists of an integrated optical Y-branch with a differential sensor principle is investigated and characterized regarding the noise resolution. …
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Towards a carbon nanotube antibody sensor
… infrared fluorescent emission after antibody binding. SWNT have potential as biosensors because of extraordinary sensitivity, lack of photobleaching, and optical activity in a near-infrared window. A SWNT sensor could provide label-free measurements of antibody concentration in a continuous …
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Biochemical and genetic analysis of nucleic acid-protein interactions
… coli is a small, sequence-specific DNA-binding protein. The specific and nonspecific DNA binding constants were determined by gel mobility shift assays. The binding constant of IHF for the H$\sp\prime$ site in $\lambda$ attP is 6.8 $\times$ 10$\sp8$ M$\sp{-1}$ and the nonspecific binding …
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Development of enforced stacked intercalators that target trinucleotide repeat mismatches in DNA
… mismatches. Interestingly, we noted that mode of binding toward CTG mismatched sites appeared to be cooperative. Due to its limited water solubility, we attempted to develop further derivatives. We took into consideration these observations to develop and improve our acridine-base ligands in the …
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Targeting Tau Aggregation for the Diagnosis and Treatment of Alzheimer’s Disease
… high concentrations of potentially confounding binding sites presented by beta-amyloid plaques. Inhibition of tau aggregation using small molecules is a promising strategy for the treatment of tauopathies, but the field lacks an understanding of the inhibitory mechanism of these …
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Molecular and Biofunctional Modification of Conformal POEGMA Bottlebrush Coatings and Applications Toward In Vitro Diagnostics
… their bio-inertness, ability to eliminate nonspecific binding events and stabilize biorecognition elements make them attractive candidates as biosensing surfaces. This dissertation describes the use of “nonfouling” (protein- and cell-resistant) nanoscale polymer bottlebrush films derived …
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Single molecule fluorescence spectroscopy of ClpXP-mediated substrate degradation
… polyethylene glycol (PEG) remove unwanted nonspecific binding of substrates, providing specific immobilization of ClpXP protease on the glass surface illuminated by total internal reflection fluorescence (TIRF). Cy3-labeled engineered substrates containing nondegradable GFP in the prescence …
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Explorations of Thermus thermophilus HB8 Transcription Factors TTHA1437 and TTHA1719 using the Combinatorial Selection Method REPSA
… for identifying and validating consensus binding sequences in <em>T. thermophilus </em>HB8 with previously studied TFs. Here REPSA was explored as a technique and method for identifying TTHA1437 and TTHA1719 TFs in <em>T. thermophilus </em>HB8. TTHA1437 REPSA results showed a promising DNA …
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Preparation and Use of Bovine Jejunal Brush Border Membrane Vesicles to Investigate Amino Acid Uptake (Nutrient, Ruminant, Absorption, Cattle, Transport)
… active intravesicular space and was not due to nonspecific binding of the substrate to vesicular membranes. Vesicles exhibited sodium dependent and independent substrate uptake pathways and were able to discriminate between substrate stereoisomers for uptake. Major differences were not detected …
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A Capillary-Based Microfluidic System for Immunoaffinity Separations in Biological Matrices
… by the direct capture approach, but displayed nonspecific binding of human plasma matrix components that limited the useful assay range. Capillary sandwich assays of CRP in human serum and cerebrospinal fluid were performed using both capture and detection antibodies. The detection antibody was …
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Enzyme-linked immunosorbent assay for red cell antibody quantitation.
… of red cell antibodies of any specificity. Binding of negatively charged reagent red blood cells (RBC) was achieved in microplate precoated with poly-L lysine (PLL). The optimum concentrations of PLL and RBC were between 10 and 20 mg/ml and 0.5% volume for volume, …
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Discovery of novel highly selective Rho kinase (ROCK) inhibitory peptides and evaluation of their biological activities
… type I which compete with ATP to bind to the ATP binding site on catalytic domain of ROCK in its active conformation and since the ATP binding site is highly conserved in kinases, these inhibitors can cause many side effects associated with their nonspecific binding and inhibition of other kinases …
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Multiplexed angiogenic biomarker quantification on single cells
… To enable this quantification, I reduce nonspecific binding between Qdot-conjugated antibodies and cells, identify optimal labeling conditions, and establish that 800 – 20,000 is the dynamic range where accurate Qdot-enabled quantification can be achieved. Through these optimizations we …
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IN-VITRO METABOLISM AND PROTEIN BINDING OF 5-HMF, A POTENTIAL ANTISICKLING AGENT
… in-vitro studies were done to characterize 5-HMF binding kinetics with human Hb and albumin (HSA). Methods. NAD+ reduction was monitored at 340 nm in human hepatic cytosol for 5-HMF (26 mM) and prototypical ADH and ALDH substrates in the presence or absence of their inhibitors. Furthermore, …
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Cooperative Assembly of Terminase and Integration Host Factor at the Packaging Initiation Site of Bacteriophage Lambda
… is complex due to the multiple terminase and IHF binding elements within cos. Therefore, I dissected the cos site into individual specific and nonspecific IHF binding sequences, and analyzed the relevant protein affinities for these subsites as well as for (1) the full-length cos site and (2) a …
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Decreased Triiodothyronine Binding to the Hepatic Nuclear Thyroid Hormone Receptor in the Diabetic Mouse
… the controls. Triiodothyronine (T<sub>3</sub>) binding studies at 4°C using nitrocellulose filtration to separate free T<sub>3</sub> from receptor bound T<sub>3</sub> demonstrated an apparent dissociation constant of (1.3 ± 0.8) x 10<sup>-10</sup> M for controls which was significantly less than …
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Engineering nanomaterials for molecular imaging and targeted therapy
… dot with minimal hydrodynamic size, negligible nonspecific interactions, chemical functionality and long stability for imaging cells and tissues. Toward this goal, a new polymer surface coating methodology is developed for making small (7.4-12 nm), stable (over months in storage), and …
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Fret Studies of DNA Binding By Lambda Cro Variants
… to protein-specific folding, assembly and binding reactions. For many model circuits protein dimerization and DNA binding are fast relative to the time scale of transcription and translation and thus amenable to modeling by statistical thermodynamics. In these cases, the distribution of …
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