Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Nonhomologous end joining"”.
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Nonhomologous end-joining: TDP1-mediated processing, ATM-mediated signaling
This thesis investigates two separate features of nonhomologous end-joining (NHEJ) DNA repair: end processing, and DNA repair kinase signaling. DNA end processing was investigated in a mouse model of hereditary spinocerebellar ataxia with axonal neuropathy (SCAN1), a congenital neurodegenerative …
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Investigation of Ring Finger Protein 8 in Mammalian Physiology and Pathogenesis
… components of homologous recombination (HR) and nonhomologous end joining (NHEJ) repair machineries into DSB-induced foci. To examine the in vivo functions of Rnf8 during mammalian development and determine the effects of its deficiency on the development of various diseases, we generated Rnf8 …
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Stabilization of Topoisomerase 2 Mutants Initiates the Formation of Duplications in DNA
… subunit forms a phosphotyrosyl bond with the 5’ ends of the break, and this DNA-protein intermediate is called a Top2 cleavage complex (Top2cc). Following the passage of an intact duplex, Top2 re-ligates the DNA and is released to restore genome integrity. Top2cc stabilization by chemotherapeutic …
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Evaluation of 64Cu-ATSM in cell culture for potential use as a radiotherapy agent
… (CHO) cell lines proficient and deficient in DNA nonhomologous end joining (NHEJ) DNA repair (10B2 and xrs-5 cells, respectively) were treated with 64Cu-ATSM. Colony formation assay results show similar cell survival for both cell lines treated with similar activities of 64Cu-ATSM, indicating cell …
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Understanding The Repair Mechanisms at Ionizing Radiation-induced Damage in The Human Genome
… breaks (DSB) with unligatable dirty ends, along with more frequent oxidized bases and single-strand breaks (SSB). While nonhomologous end joining and homologous recombination are major DSB repair pathways which have been extensively characterized over the past decades, contribution of …
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Effect of Werner and Bloom Helicase Deficiencies On Dna Double-Strand Break Repair
… events that occur in cells. Causes can include endogenous agents such as reactive oxygen and nitrogen species, exogenous agents such as ionizing radiation and ultraviolet light, or other sources such as processing of spontaneous DNA lesions or collapse of stalled replication forks. The RecQ …
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Investigating the Molecular Mechanisms Underlying Ku Essentiality in Human Cells
… and Ku80, and is well- known for its role in nonhomologous end-joining DNA repair. Ku has also been associated with noncanonical functions in the cell for which its mechanisms are not fully understood such as its roles in telomere maintenance, the cell cycle, and transcriptional regulation. …
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Analysis and confirmation of the results of a yeast two-hybrid screen carried out to identify proteins that interact with drosophila XRCC2
… are repaired by two different mechanisms. The nonhomologous end-joining pathway can bring together the broken ends and join them, usually with the loss of some nucleotide sequence. A second pathway, homologous recombinational repair (HRR), is equally important. This repair process utilizes the …
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Functional Analysis of the Human Telomeric Protein TRF2
… of these structures and appearance of end-to-end fusions visible in metaphase and anaphase cells. Fusion formation was accompanied by the loss of Goverhang, but not by the loss of double stranded telomeric DNA, which can be documented to persist at the sites of fusions. Fusions were …
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Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage
… regulation of RPA and p53 interaction by DNA-dependent protein kinase (DNA-PK), ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) in homologous recombination (HR). HR and nonhomologous end joining (NHEJ) are 2 distinct DNA double-stranded break (DSB) repair pathways. Here, we …
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A histone H4 code regulates 53BP1-mediated responses to DNA damage
… are relatively stable and are established independently of DNA damage, we propose that reversible changes in the levels of K16ac co-modification act like a dynamic switch to modulate DDR responses mediated by K20me2. Analyses of the interaction of the K20me2 binding domain of 53BP1 with H4 …
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Functional Analysis of the Yku Complex in Telomere Length Regulation
… repair of DNA double strand breaks (DSBs) via nonhomologous end-joining (NHEJ). Interestingly, Ku has been shown to bind to the native chromosome ends. It contributes to the maintenance of wild type telomere length and, moreover, has been implicated in the protection of the telomeres from …
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Functional characterization of poly(ADP-ribose) dependent ubiquitin E3 ligase in the DNA damage response
… repair 3 1. Homologous recombination (HR) 4 2. Nonhomologous end joining (NHEJ) 5 C. Ubiquitylation mediated DNA damage response signaling 6 1. Ubiquitylation 6 2. Post-translational modification mediated DNA damage response 7 3. RNF4 functions in the DNA damage response 8 4. Heterochromatin …
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Studies of site-specific DNA double strand break repair in plants
… with donor T-DNA, and several independent germinal recombinants have been recovered from both strategies with gene targeting frequency of 0.3-2.0X10<sup>(-3)</sup>. We also attempted gene targeting by ectopic recombination, with donor homology provided by the concomitant integrated …
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ARTEMIS AND METNASE MEDIATED PROCESSING OF 3΄-BLOCKED DNA LESIONS: ROLE IN RADIO/CHEMORESISTANCE AND DNA REPAIR
… breaks (DSB) with chemically modified end-termini are the most significant lesions resulting from radio/chemotherapeutic intervention of cancer and non homologous end-joining (NHEJ) factor Artemis nuclease has been implicated in the repair of such breaks. To examine whether the …