Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Non-viral delivery"”.
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Novel non-viral delivery systems for cancer gene therapy
The non-viral gene delivery systems dendrimers have been widely studied for cancer therapy. However, due to lack of targeting efficacy, their therapeutic applications are still limited. In this present study, our aim was to investigate the efficacy of four formulations of modified generation- 3- …
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Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering
… was to develop a collagen scaffold for the non-viral delivery of the gene encoding for Glial Cell-Derived Neurotrophic Factor (GDNF); hence to provide a local, long-term release and overexpression of GDNF via transfection of cells seeded into the scaffold or endogenous cells. The first part …
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Endocytosis of therapeutic macromolecules in tumor cells - Mechanistic aspects of the proteoglycan receptor function
… of nucleic acid based therapies in the clinic. Viral delivery methods have been demonstrated to efficiently deliver nucleic acids, but also to be associated with severe, occasionally life threatening, immune reactions. Non-viral delivery methods are, so far, not sufficiently efficient for use in …
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Investigation of the immunostimulatory activity and vaccine potential of lipid encapsulated plasmid DNA and oligodeoxynucleoties
… and examining their ability to act together as a non-viral DNA vaccine in attempt to address the shortcomings of current DNA vaccine approaches. One focus of this thesis concerns investigating the immunostimulatory activity of LN formulations of CpG-ODN and pDNA. It is shown that despite dramatic …
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Engineering M13 Bacteriophage Nanoplatforms for Diagnostic and Therapeutic Applications
… protein subunits organized in a filamentous viral capsid; 900nm in length and 6nm in diameter. All M13 capsids are amenable to mutation and can be tuned for the binding and nucleation of inorganics and nanoparticles, and for the expression of ligands, functional moieties, and even enzymes. To …
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Design of novel delivery systems to probe alternative routes of administration for a self-amplifying RNA rabies vaccine
… in situ antigen expression, mimicking a real viral infection hence eliciting robust humoral and cellular-mediated immune responses. RNA vaccines therefore represent a versatile tool to fight infectious diseases and emerging pathogens effectively and rapidly. Furthermore, the antigen can be …
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Development of a nanoHydroxyapatite-collagen composite scaffold for bone tissue engineering
… Chapter 2 of the thesis, a technique to produce non-aggregating nano-sized particles (<100 nm) of HA was developed using a novel, dispersant-aided synthesis procedure.<br/><br/>In Chapter 3, the incorporation of the synthesised nHA into the collagen scaffolds was investigated. The optimised …
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The design of cyclodextrins for delivery of siRNA - a structure-activity relationship
Previously non-viral delivery of therapeutic nucleic acids (NAs) has been achieved for the treatment of liver disease and in the case of the COVID-19 vaccine. The delivery vector in both applications was a lipid-base nanoparticle (LNP). To expand the therapeutic application of NAs to treat more …
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Non-covalent Intermolecular Interactions in Polymer Design: Segmented Copolymers to Non-viral Gene Delivery Vectors
Non-covalent intermolecular interactions play a large role in determining the properties of a given system, from segmented copolymers to interactions of functionalized polymers with non-viral nucleic acids delivery vehicles. The ability to control the intermolecular interactions of a given system …
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Understanding The Visual And Auditory Defect In Ush2a Mouse Model
… and attempting gene therapy with conventional viral delivery is not easily attainable due to limited payload capacity of commonly used Adeno-associated viruses (AAV) ( <4.7 kbp.) To understand the mechanism underlying hearing and visual impairments, a knock in (KI) mouse model (Ush2adelG/delG) …