Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 84 for “"Non-viral"”.
-
Non-viral drug delivery systems for immune modulation
Biodegradable polymer particles have diverse applications in drug delivery. The main objective of this thesis was to apply these delivery systems to modulating the immune system. We optimized particle formulations for the delivery of three novel immune modulating compounds, small inhibiting RNA, …
-
Novel non-viral delivery systems for cancer gene therapy
The non-viral gene delivery systems dendrimers have been widely studied for cancer therapy. However, due to lack of targeting efficacy, their therapeutic applications are still limited. In this present study, our aim was to investigate the efficacy of four formulations of modified generation- 3- …
-
Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
Non-viral gene delivery agents, such as cationic polyelectrolytes, are attractive replacements to viruses due to the absence of potential immunogenic risk and the ability to tune their macromolecular structure. Although non-viral vectors possess numerous design advantages, several investigators …
-
Understanding the biological barriers to Jurkat Transfection using non-viral peptides
The overall objective of this thesis is to examine the feasibility of RALA peptide to manufacture CAR-T cells. <br/><br/>As primary T-cells are hard to transfect, the key barriers to the transfection of primary T-cells should be explored for further rational designs of transfection agents. The low …
-
Elucidation of Design Criteria for Non-Viral Gene Delivery Vector Development
… endosomal pathway, dissociation of DNA from its non-viral carrier, migration of DNA to the nucleus, nuclear entry and finally transcription all stand as obstacles to gene delivery. The research presented in this thesis addresses the issue of gene delivery efficiency by studying vector …
-
Cationic, α-helical polypeptides for cell penetration and non-viral gene delivery
… crucial towards gene therapy. Compared with viral vectors, non-viral gene delivery vectors allow the safe delivery of genetic materials with less inherent adverse reactions such as immunogenicity, unexpected viral replication and recombination, and oncogenicity. However, the non-viral vectors …
-
Photo-responsive degradable poly (beta-amino ester) toward non-viral gene delivery
… ester) (PBAE) is one of ideal candidates for non-viral gene therapy due to its desired gene delivery efficiency, biocompatibility, and biodegradability. In order to further improve the gene delivery efficiency of PBAE by facilitating the intracellular DNA dissociation, we herein report the …
-
Quantitative analysis of non-viral gene therapy in primary liver culture systems
… novel combination therapies for cancers and viral infections, and offer a new and effective platform for next generation vaccines. However, after more than three decades of research and development efforts, clinical success has yet to be realized. Successful delivery of DNA is a crucial first …
-
Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering
… was to develop a collagen scaffold for the non-viral delivery of the gene encoding for Glial Cell-Derived Neurotrophic Factor (GDNF); hence to provide a local, long-term release and overexpression of GDNF via transfection of cells seeded into the scaffold or endogenous cells. The first part …
-
Synthesis and evaluation of novel non-viral gene delivery systems for prostate cancer targeting
… double than that observed in cells treated with non-targeted dendriplex. The anti-proliferative efficacy of DAB-Lf dendriplex encodingTNFα, TRAIL, or IL-12 was significantly improved compared with unmodified DAB dendriplex. In vivo, intravenous injections of DAB-Lf dendrimer complexed with …
-
Biochemical Investigation of the Intracellular Trafficking of Non-Viral and Hybrid Gene Therapy Vectors
We also used reverse transcriptase inhibitor 3'-azido-3'-deoxythymidine (AZT) applied of post-transfection time increments to determine the timeframe of reverse transcription, thereby estimating endosomal escape of VLP. MLV were observed to achieve reverse transcription rapidly, with more than half …
-
Effect of uptake pathway on non-viral gene delivery in vitro and in vivo
Efficient non-viral gene delivery often involves the conjugation of a cell-specific ligand to the vector, which directs the vector to its intended target through binding to a cellular receptor followed by internalization via endocytosis. However, little is known in terms of how the various …
-
Understanding Non-viral Nucleic Acid Delivery Vehicles with Different Charge Centers and Degradation Profiles
Different structures of non-viral cationic polymer delivery vehicles, including charge center type, molecular weight and degradability, could significantly affect toxicity, release of nucleic acid and transfection efficiency. Poly(glycoamidoamine)s (PGAAs) contained different carbohydrate and …
-
Non-covalent Intermolecular Interactions in Polymer Design: Segmented Copolymers to Non-viral Gene Delivery Vectors
Non-covalent intermolecular interactions play a large role in determining the properties of a given system, from segmented copolymers to interactions of functionalized polymers with non-viral nucleic acids delivery vehicles. The ability to control the intermolecular interactions of a given system …
-
Regulated release of a novel non-viral gene delivery vector from electrospun coaxial fiber mesh scaffolds
The development of novel strategies for tissue engineering entails the evolution of biopolymers into multifunctional constructs that can support the proliferation of cells and stimulate their differentiation into functional tissues. With that in mind, biocompatible polymers were fabricated into a …
-
Investigation of barriers to non-viral gene delivery and design of novel polymer-based gene delivery systems
… significant challenge facing gene therapy today. Viral vectors remain the dominant approach for addressing the delivery problem; however, concerns regarding the safety of viral vectors have resulted in an increasing interest in non-viral vectors. Non-viral vectors offer the promise of improved …
Page 1 of 5