Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"Non-structural proteins"”.
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Impact of mutations in non-structural proteins on SARS-CoV-2 replication
… well as recent advancements in understanding the structural and functional implications of mutations in viral proteins. The prevailing belief is that SARS-CoV-2 originated from a zoonotic transmission involving bats as the natural reservoir hosts, with an unknown intermediate host facilitating …
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Investigating the role of non-structural genes in the genotype and phenotype of yellow fever vaccine strain 17D
… substitutions with nine substitutions in the structural proteins of the virus and the remaining eleven in the non-structural proteins. Despite the long-term use and success of the 17D vaccine, it remains unknown which of the 20 residues contribute to its stably attenuated phenotype. By …
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Purification and Characterization of Simian Virus 40 Large T Antigen
… Large T antigen is one of two viral-coded non-structural proteins, and is produced early in productive infection and continuously in transformed cells. Several lines of evidence indicate that large T antigen of SV40 is involved in the initiation of viral DNA replication during productive …
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The Influence of Nucleic Acids of Yolk Origin on the Growth and Development of the Avian Yolk Sac
… are dependent only on the synthesis of specific structural and non-structural proteins with all else being spontaneous processes, one is still faced with the problem of differential initiation of protein synthesis with a common code at its base.
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Understanding the determinants for substrate recognition, regulation of enzymatic activity and the development of broad-spectrum inhibitors of coronavirus 3-chymotrypsin-like proteases
… for viral polyprotein processing to release non-structural proteins that form the replicase complex machinery for viral genome replication. Due to its indispensable role in coronaviral replication, 3CLpro is an attractive drug target. Moreover, high sequence conservation in the vicinity of …
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Analysis of the interaction between the 2C protein of parechoviruses and lipid droplets
Picornaviruses are non-enveloped, positive sense single-stranded RNA viruses which cause many diseases ranging from slight illness to fatal meningitis and encephalitis. There are no vaccines against most picornaviruses so drugs need to be developed. Human parechoviruses (HPeVs) are being …
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The analysis of a conserved RNA structure in the 3D polymerase encoding region of Human Parechovirus 1
… a single polyprotein that is cleaved into structural and non-structural proteins. Analysis of one region of the genome (encoding the polymerase, 3Dpol) shows that some codons are perfectly conserved, suggesting functions in addition to protein coding. This region seems to fold into an RNA …
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<i>Maize fine streak virus</i> (MFSV) gene expression and protein interaction
… The MFSV genome is a 13,782 nucleotide, non-segmented, negative-sense RNA that encodes five core structural proteins: the nucleocapsid protein (N), the phosphoprotein (P), the polymerase (L), the matrix protein (M) and the glycoprotein (G) and two non-structural proteins, MFSV 3 and 4. …
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Coronavirus Replicase Proteins: Multifunctional Mediators of Replication and Innate Immunity Evasion
… RNA viruses. The majority of the RNA encodes non-structural proteins (nsps) that are translated as a large polyprotein, which is cleaved by the papain-like (PLP) and picornavirus 3C-like (3CLpro) proteases. The nsps modify host membranes to produce double membrane vesicles (DMVs) upon which …
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Novel approaches to enhance the protective immune responses of vaccines against Porcine Reproductive and Respiratory Syndrome Virus
… we cloned and expressed all known PRRSV structural and non-structural proteins and examined which protein(s) is involved in SLA-I downregulation. Our results identified the newly-discovered nonstructural protein Nsp2TF of PRRSV as the main mediator in down-regulating SLA-I expression. We …
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Strucutre-function studies of the venezuelan equine encephalitis virus 5'UTR promoter element and its role in attenuation of the virus
RNA structural elements play critical roles in several viral processes. An \r\nattempt to elucidate the role of one such RNA structural element encoded by the 5’ \r\nUntranslated Region (5’UTR), in regulating virus replication and attenuation of \r\nVenezuelan equine encephalitis virus (VEEV) is …
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The production of foot-and-mouth disease virus-like particles in the plant Nicotiana benthamiana: a potential candidate vaccine for foot-and-mouth disease
… Furthermore, inactivated virions present non-structural proteins (NSPs) and thus cannot be distinguished from the infectious virus by imported ELISA kits that utilise the NSPs as coating antigens and conventionally produced detecting antibodies. We aimed to use recombinant constructs …
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Identifying B-cell epitopes of African horse sickness virus serotype 4 recognised by antisera of immunised horses
… sickness is an infectious, insect-borne but non-contagious disease of equids responsible for more than 95% mortality in naïve horses. The African horse sickness virus has ten segments of double stranded RNA encoding seven structural and three non-structural proteins. There is currently no …
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Transcription and encapsidation in parvoviruses LuIII and bovine parvovirus
… rodent parvoviruses MVM and H-1. The presence of non-identical LuIII termini demonstrated that identical ends are not required for the encapsidation of both DNA strands with equal frequency, as suggested for parvoviruses B19 and AAV. An infectious genomic clone of LuIII was constructed and …
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Cross-protection from St. Louis encephalitis virus and Usutu virus disease by human West Nile virus convalescent plasma in mice
… polyprotein is produced, consisting of three structural and seven non-structural proteins. These proteins function in virus binding to the cell membrane, entry into cells, replication, immune evasion, and the production of new virus progeny. Typically, these viruses are maintained in a …
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Engineered Replicases, Synthetic DNA, and Long-Read Sequencing Quality Control for Adeno-Associated Virus Production
… the most expensive medicinal products. AAV is a non-pathogenic dependovirus with a bi-phasic lifecycle, and rAAV production systems inherit a complex biology from the wild-type virus. The viral replicases (Rep), four non-structural proteins of AAV (Rep40, 52, 68, and 78), control the complex …