Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Non-canonical amino acids"”.
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A multiplexed approach for quantitative profiling of the translatome using bioorthogonal non-canonical amino acids
… newly translated proteins, biorthogonal non-canonical amino acid tagging (BONCAT), and adapt it for robust quantitative multiplexing analysis. In the BONCAT method, cells are pulsed with azidohomoalanine (Aha), a methionine analog that contains an azide functional group, such that …
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Genetic Code Expansion in Mammalian Cells
… are synthesized from a conservative set of 20 canonical amino acids, limiting the chemical space of biological systems. Over the last few decades, scientists have developed methods to expand the genetic code of living organisms, introducing new, non-canonical amino acids with diverse …
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Identification and Evolution of New Orthogonal Aminoacyl-tRNA Synthetase/tRNA Pairs for Genetic Code Expansion
… biology aiming to expand the repertoire of amino acids which can be incorporated into proteins in vivo. A central challenge in expanding the genetic code of cells to incorporate non-canonical amino acids is the scalable discovery of aminoacyl-tRNA synthetase (aaRS)–tRNA pairs (the components …
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Engineering of the microviridin post-translational modification enzymes for the production of synthetic protease inhibitors
… variants, including variants carrying non-canonical amino acids (ncAAs). The microviridins are a family of RiPPs characterized by their atypical tricyclic structure composed of lactone and lactam rings, and their activity as serine protease inhibitors. The generalities of their …
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Synthesis, Characterisation and Evolution of Escherichia coli with a Compressed Genetic Code
… code. Such liberated codons can be reassigned to non-canonical amino acids to equip proteins with novel functions. Furthermore, recoded organisms are genetically distinct from all other lifeforms and may therefore prove useful for biocontainment and viral resistance. Chapter I outlines our …
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Delivery of biomolecules into mammalian cells using anthrax toxin
… and intracellular components. Recently, a non-toxic delivery platform derived from anthrax lethal toxin has been developed to overcome this challenge for the delivery of biomolecules into the cytosol of mammalian cells. The PA/LFN delivery platform has been used to deliver over 30 known …
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Expanding the structural diversity of the RiPP class of natural products
… Despite starting out as linear chains of amino acids, RiPP natural products acquire structural diversity ranging from small molecules like microcin C7 to 48-mer polytheonamides with varying post-translational modifications installed by biosynthetic enzymes. These conformational …
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Orthogonal pair-directed codon reassignment as a tool for evaluating the factors affecting translation in E. coli
Proteins are polymers of amino acids that are essential for life, central to cellular function, and have applications in fields ranging from materials science to biomedicine. Proteins in nature are composed of 20 amino acids with limited variability in size and chemical properties. Expanding the …
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Exploration of the biosynthesis of lanthipeptides
… 4 describes efforts towards the incorporation of non-canonical amino acids into lanthipeptides, in order to expand the structural diversity of these natural products, tune their activities, and introduce probes for subsequent functional investigations. The work presented in this thesis provides …
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Organometallic Palladium Reagents for Polypeptide Bioconjugation and Macrocyclization
… and cyclic peptide OACs are accessible through non-exhaustive C–S arylation, leaving the active Pd(II) site intact for future functionalization to small molecules or other biomolecules. Chapter 2: Macrocyclic peptides hold immense potential for targeting “undruggable” protein-protein …
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Affinity Maturation of Peptides to Bind Protein-Protein Interfaces
… Lastly, a systematic study on the tolerance of non-canonical amino acids of characteristic chirality, steric effects, and backbone structures has been presented. Overall, these studies highlight the potential of peptide-based inhibitors and affinity selection platforms in drug discovery
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A General Platform for Ribosome Engineering
… genetic code in vitro and in vivo to assemble non-canonical polymers with this approach. However, the properties of the natural ribosome limit existing work to close analogues of the canonical amino acids. This thesis develops a directed evolution technology to engineer ribosomes in vitro. The …
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Site-Specific Labelling of the Flavivirus Envelope Protein using Genetic Code Expansion
… genetic code expansion to incorporate the non-canonical amino acid BCN-K at specific sites in the E protein. BCN-K is a versatile non-canonical amino acid that can be labelled via reactions with both tetrazine- and azide-conjugated probes. I demonstrated that BCN-K could be incorporated at …
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Acetylation regulates Thioredoxin Reductase activity and oligomerization
… system and contains the 21st genetically encoded amino acid, selenocysteine (Sec). There were multiple experimentally identified TrxR acetylation sites with an unknown effect on TrxR activity. My thesis tested the hypothesis that programmed protein acetylation will enhance the activity of TrxR1. I …