Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Nodal signalling"”.
-
Activin/Nodal Signalling Controls the Epigenome and Epitranscriptome of Human Pluripotent Stem Cells
… great progress has been made in elucidating the signalling pathways regulating pluripotency and differentiation, our mechanistic understanding of the downstream regulations is still incomplete. Moreover, studies aimed at clarifying these aspects are severely impeded by the lack of efficient …
-
The Role and Control of WNT Signalling in an HESC Model of Human Primitive Streak
… is largely controlled by WNT, BMP, and ACTIVIN/NODAL signalling. Despite detailed characterization in model organisms, the human PS and the role these pathways play in its formation and patterning remains a mystery. In this work I focused on understanding the role and control of the WNT pathway …
-
Defining the minimal and context-dependent signalling cascades regulating the maintenance of human naïve embryonic stem cells and early embryo development
… epiblast lineage. Whilst some of the signalling cascades that are fundamentally implicated in modulating the naïve gene regulatory network have been studied, our understanding of the finer details is incomplete. To date, the roles of the WNT signalling cascade remains a topic of …
-
Extracellular Signalling and Stem Cell Self-Renewal
In preimplantation mouse embryos, signalling and gene regulatory networks cooperate to determine lineage segregation, and modulating signalling in vitro allows for stem cell populations to be established from these lineages. Fibroblast growth factor (FGF) signalling triggers the differentiation of …
-
TRANSCRIPTIONAL NETWORKS VARIATIONS DURING CELL CYCLE PROGRESSION IN HUMAN EMBRYONIC STEM CELLS
… control the transcriptional activity of Activin/Nodal signalling during progression of the cell cycle in human Embryonic Stem Cells (hESCs). As a consequence, hESCs can only differentiate into endoderm in the Early G1 phase when Cyclin Ds are expressed at low levels. These results show the …
-
Morphogenesis of the early post-implantation mouse embryo
… cavity. I show that this process is dependent on signalling cues stemming from the underlying basement membrane that activate the b1-integrin signalling pathway to regulate cell polarity, ExE tissue architecture and rosette formation. In addition to the basement membrane’s role in b1-integrin …
-
An in vitro model of human yolk sac and epiblast crosstalk
Embryogenesis entails complex signalling interactions between embryonic and extraembryonic lineages. Improved embryo culture techniques have recently allowed human embryos to be cultured in vitro past the point at which they would implant in vivo, until the internationally accepted culture limit of …
-
Nodal organized cell dynamics for building tissue shapes in vertebrate gastrulation
… is a major unresolved question in biology. Nodal is a key developmental regulatory gene from the TGF-β family that induces mesoderm and controls cell movement during gastrulation. How Nodal alters cell dynamics and mechanics is not well understood. To explore Nodal’s morphogenetic role in …
-
WNT signalling in the patterning of human gastruloids
… and leads to the formation of the body plan. WNT signalling, an evolutionary conserved pathway, is known to play a crucial role during gastrulation with its dysregulation leading to various developmental abnormalities and diseases. This thesis examines the role of WNT signalling in the patterning …
-
Bovine Pre-gastrulation Development and the role Of Rauber's Layer
… little is known about the gene expression and signalling networks required for successful gastrulation in these species, particularly cattle which are also economically important. This thesis studied the morphological and gene expression events during Days 11-15 in cattle which coincides with …