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Showing 1 to 20 of 23 for “"Nitrotyrosine"”.
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In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… the in vitro selection of deoxyribozymes for 3-nitrotyrosine azido-adenylylation described in Chapter 4. Previous research had identified deoxyribozymes for tyrosine azido-adenylylation. The hydroxyl group of 3-nitrotyrosine is many orders of magnitude less reactive than that of tyrosine. In …
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Chlorinated Tyrosine Species as Markers of Inflammation: A Kinetic Study
… ring by reactive nitrogen species to produce 3-nitrotyrosine (NO2Tyr) are two such markers. Both are stable byproducts of MPO activity and are readily measurable. Some studies, however, have called into question their use as biomarkers of inflammation. Concentrations of 3ClTyr or NO2Tyr …
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Three dimensional molecular architectures for the synthesis and improved properties of high performance polymers
… backbones. We also use the detection of 3-nitrotyrosine as a probe to learn more about its physical state in solution. We further utilize the monomer above in a biocompatible post-polymerization functionalization reaction, taking advantage of the polymer's structural motif for the …
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The role of nitric oxide in pre-eclampsia
… lipid peroxides and the peroxynitrite product, 3-nitrotyrosine. An improved GC-MS method was developed to quantify total fatty acids and lipid peroxides. A new reversed phase HPLC technique for the analysis of free 3-nitrotyrosine in human plasma/serum was also developed although sample numbers …
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Amyotrophic Lateral Sclerosis: mechanism behind mutant SOD toxicity and improving current therapeutic strategies
… with peroxynitrite led to an increase level of nitrotyrosine in comparison to wild type controls. However, in the same samples there was a 2 to 10 time increase in Hsp90 nitration as compared to nitrotyrosine. The selective increase is likely due to the binding of Hsp90 to Zn deficient and Q SOD …
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Persistent Oxidative Damage Accounts for the Origin of Mutations Observed in Uterine Pathology
… to peroxynitrite, which can also generate nitrotyrosine by interaction with proteins. We have also detected nitrated proteins in benign human uterine tissue, and for the first time identified the predominantly nitrated protein as ACTBL2. Leiomyomas are frequently observed and are a common …
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Mechanistic investigations of the radical initiation pathway of class I ribonucleotide reductase from Escherichia coli
… acid. Thus far, we have replaced Y356 with 3- nitrotyrosine (NO2Y), 2,3-difluorotyrosine (2,3-DFY), 2,3,5-trifluorotyrosine (2,3,5-TFY), and 2,3,5,6-tetrafluorotyrosine (2,3,5,6-TFY). Titrations of the NO2Y-R2 construct allowed us to show that the pKa of the putative residue on the PCET pathway …
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Mechanisms of imporvement following functional inhibition of nitrosative stress after brun and smoke-induced acute lung injury
… measured in the lung parenchyma as well as 3-nitrotyrosine and protein carbonyl formation (indices of RNS and ROS generation, respectively). In our murine study, treatment with the H2S donor significantly reduced the pro- inflammatory cytokine level and increased the anti-inflammatory cytokine …
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Post-Translational Modification of Proteins via Ambient Air Pollutants and Endogenous Reactive Species
… structure and function. The formation of 3-nitrotyrosine (NTyr) and dityrosine (DiTyr) upon reaction of proteins with ROS/RNS are two common PTMs studied due to their stability and irreversibility, as well as their ability to enhance the allergenicity of pollen allergens upon formation. Many …
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Cardiovascular and renal responses to continuous, moderate and high-intensity interval exercise in mid-spectrum CKD.
… Serum and plasma samples were measured for nitrotyrosine (ᶟ¯NT) asymmetric dimethyl arginine (ADMA), paroxonase-1 (PON-1), total antioxidant capacity (TAC), and creatinine. Urine samples were measured for epidermal growth factor (uEGF) and creatinine and the uEGF/uCr ratio was calculated as …
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Modulation of the response to cisplatin by nitric oxide and reactive oxygen species in melanoma cells
… tumors express nitric oxide (NO) synthase and/or nitrotyrosine are often faced with poor prognosis. Moreover, it has been shown that NO produced by melanoma cells sustains lower sensitivity to cisplatin toxicity in vitro. Because inflammatory products such as NO and reactive oxygen species (ROS) …
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THE ROLE OF CAVEOLAE IN THE FORMATION OF ABDOMINAL AORTIC ANEURYSMS
… then their WT counterparts as assessed by anti-nitrotyrosine staining. Next we examined the characteristics of early AAA formation in wild-type mice. We found caveolae associated proteins, endothelial nitric oxide synthase (eNOS) and NADPH oxidase 2 (Nox2), were upregulated in early AAA …
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Tyrosine nitration and altered protein pool levels of glyceraldehyde-3-phosphate dehydrogenase following ischemia/reperfusion: The influence of aging in glycolytic skeletal muscle
… selected following 2D gel electrophoresis and nitrotyrosine western blotting of\r\nyoung and old muscle lysate was identified as GAPDH by mass spectrometry. We\r\ncompared tyrosine nitration over the time course of reperfusion. While total tyrosine\r\nnitration does not increase in the I/R …
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Connexin43 as a novel therapeutic target in age-related macular degeneration
… increased levels of the oxidative stress marker, nitrotyrosine. Intravitreal injection of a Cx43 mimetic peptide was used to modulate the function of Cx43 hemichannels in the AMD animal model. A single dose treatment showed a trend toward improvement in function of both rod and cone pathways when …
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CAVEOLAE AS SPATIO-TEMPORAL COMPARTMENTS FOR ROS/RNS GENERATION AND NITROXIDATIVE STRESS SIGNALING
… immunoprecipitation of cell lysates with an anti-nitrotyrosine antibody revealed Src-family kinases (SFK) in the precipitated fraction. Moreover, SFK nitration was lost in cells depleted of caveolin-1. Given that SFK nitration is associated with enzyme activation, cells were pretreated with PP2 to …
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Next generation approaches toward engineering therapeutic proteases
… that selectively hydrolyzes peptides after 3-nitrotyrosine while effectively discriminating against similar peptides containing unmodified tyrosine, sulfotyrosine, phosphotyrosine and phosphoserine. The isolation of protease variants that can discriminate between substrates based on the …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… It also increased the oxidative stress marker nitrotyrosine but did not alter the extent of EdU nuclear staining nor the expression of the receptor of advanced glycation end-product. The antioxidant n-acetyl cysteine decreased the fraction of hyperglycaemia-induced TUNEL-positive cells and …
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Development of a Workflow to study Neuronal Injury In Vivo in the Retina and In Vitro in Collagen Hydrogels
… group than the sham group (P < 0.001). Nitrotyrosine, an indicator of oxidative stress, was elevated in the ganglion cell layer of the blast-exposed group. Müller cells of the retina express glial fibrillary acidic protein (GFAP). GFAP expression was similar between the two groups. The …
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Dietary intervention with whole flaxseed or vitamin D has beneficial effects on leiomyomas in the oviduct of the laying hen
… Willebrand Factor), NF-κB p65, oxidative stress (nitrotyrosine), cellular senescence (Sudan Black B), and prevalence of mast cells (Toluidine Blue) were performed on fibroid tissue sections. Quantitative RT-PCR was performed to determine changes in expression levels of mRNA for collagen I, …
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Natural history and pathogenesis of Takotsubo cardiomyopathy
… looking at the local and systemic rise in the 3-nitrotyrosine (3-NT) as a footmark of ONOO⁻ formation. As a part of clinical investigations, we evaluated the role of RV involvement in early hemodynamic derangement. We performed a meta-analysis to assess the impact of “secondary” TTC, male gender, …
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