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Showing 1 to 9 of 9 for “"Nitrile Hydratase"”.
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The crystal structure of a mutant nitrile hydratase
Nitrile hydratases can be used as industrial biocatalysts. They catalyze the conversion of nitriles to their corresponding amides. These industrial biocatalysts have recently gained attention due to the economic production of industrial commodities such as acrylamide and nicotinamide (Thomas et …
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Catalytic Mechanism and Maturation of the Metalloenzyme Nitrile Hydratase
<p>Nitrile hydratases are metalloenzymes that catalyze the hydration of nitriles to their corresponding amides in a specific manner at ambient pressures and temperatures at neutral pH. Traditional industrial methods require high temperature and pressure, extreme pH, and heavy metals. NHases are …
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The structure, function and engineering of a thermostable nitrile hydratase
Nitrile hydratases (NHases) are enzymes that catalyse the conversion of organocyanides to amides via a non-hydrolytic hydration reaction. They are industrially relevant enzymes, currently used in the manufacture of nicotinamide and acrylamide. The target of this study belongs to the thermophilic …
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The relationship between structure and thermostability of a nitrile hydratase from Goebacillus pallidus RAPc8
Nitrile hydratases (NHases) are very important biocatalysts for the enzymatic conversion of nitriles to industrially important amides such as acrylamide and nicotinamide. An “ideal” NHase should fulfil several essential criteria including, high substrate conversion rates, being able to tolerate …
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Investigation of the determinants of thermal stability of the nitrile hydratase from Geobacillus pallidus RAPc8
… to enhance their application in industry. The nitrile hydratases group of enzymes catalyse the hydrolysis of nitriles to amides using an exothermic catalytic mechanism. Understanding and applying specific amino acid residue mutations at specific regions in protein structures has been important …
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An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8
Nitrile hydratases (NHases) are bacterial metalloenzymes that catalyze the hydration of nitriles to their corresponding amides. The enzymes have been found in several microorganisms and participate in the metabolism of nitrile compounds as source of carbon and nitrogen. The commercial use of NHases …
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X-ray Spectroscopic Studies of Model Compounds With Relevance to Nickel Superoxide Dismutase, Nitrile Hydratase and Copper Related Neurodegenerative Disorders
… The next system studied, CoNHase (cobalt nitrile hydratase), was investigated to determine the active form and also the extent of oxidation of the Cys residues. A metallopeptide mimic was synthesized and then sequentially oxygenated to its active form, a sulfinate (SO<sub>2</sub>) and a …
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Ph-dependence of the quaternary structure of the cyanide dihydratase from bacillus pumilus
Nitrilases are moderately ubiquitous nitrile/cyanide-degrading enzymes, found in both eukaryotes (animals, fungi, plants) and prokaryotes (archaea, bacteria) which catalyse the condensation and hydrolysis of a wide range of non-peptide nitrile substrates and are involved in …
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Screening for novel cold-adapted nitrile hydratases in Antarctic metagenornic libraries
Nitrile hydratases (NHase) catalyse the hydrolysis of nitriles to their corresponding amides, the first step in the nitrile degradation pathway. The second step is the conversion of the amide to a carboxylic acid, catalysed by an amidase. The NHases published to date have been isolated from …