Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"Nicotinamide adenine dinucleotide (NADH)"”.
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Label-free multiphoton microscopy for imaging transient metabolic dynamics in living cells and tissue
… lifetime imaging microscopy (2P-FLIM) of reduced nicotinamide adenine dinucleotide (NADH). This autofluorescent co-enzyme is involved in both aerobic and anaerobic metabolic processes which can be differentiated utilizing this advanced imaging approach. This thesis first presents a study of cell …
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Metabolic-response assessment of metastatic murine breast cancer in 2D and 3D cultures using intrinsic NADH as a natural biomarker
… 2P-fluorescence anisotropy of the reduced nicotinamide adenine dinucleotide (NADH) in metastatic murine breast cancer cells 4T1. In addition, we investigated the cellular metabolic response of 4T1 cells in 2D monolayer and 3D collagen matrix cultures to drug treatment using two novel …
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Mitochondrial metabolism in cancer transformation and progression
… I found that impaired utilisation of reduced nicotinamide adenine dinucleotide (NADH) by the mitochondrial respiratory chain leads to cytosolic reductive carboxylation of glutamine as a new mechanism for cytosol-confined NADH recycling supported by malate dehydrogenase 1 (MDH1). This metabolic …
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Thin films of non-peripherally substituted liquid crystalline phthalocyanines A
… oxidised compounds and biological cofactors nicotinamide adenine dinucleotide (NADH) and L-ascorbic acid (vitamin C) were carried out using optical absorption spectroscopy. Thin films of a non-peripherally octyl substituted LC lead phthalocyanine was exposed to 99.9 % pure hydrogen sulfide …
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Monitoring glycation through the intrinsic fluorescence of biological fluorophores
… experience glycation, and the cofactor reduced nicotinamide adenine dinucleotide (NADH) is also known to be affected in some way. This work aims to establish if the intrinsic fluorescence of these compounds could be used to monitor the impact of glucose, and thus offer an alternative method to …
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Metabolic and molecular responses to interspecies hydrogen transfer between Ruminococcus albus and Wolinella succinogenes
… HydABC, couples proton reduction using nicotinamide adenine dinucleotide (NADH) to proton reduction using reduced ferredoxin (Fdred), producing molecular hydrogen: 3 H+ + NADH + Fdred → 2 H2 + NAD+ + Fdox. HydA2, a ferredoxin-dependent [FeFe]-hydrogenase, reduces protons to molecular …
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Characterization of alternative enzymes in bacterial respiration
… terminal electron acceptors. The type II NADH dehydrogenases (NDH-2s) represent one such alternative enzyme. They are membrane-associated enzymes that oxidize NADH and reduce quinone, similar to the canonical Complex I in mitochondria. However unlike the Complex I they do not contribute to …
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Investigating xanthine oxidase toxicity models in cultured cerebellar granule neurons
… effect of the combination of XOR and the reduced nicotinamide adenine dinucleotide (NADH) in cell cultures is much less studied. NADH is another oxidizable substrate for XOR that binds to a different site on the enzyme from that of X binding. The aim of this project was to investigate some aspects …
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Myoglobin redox form stabilization: role of metabolic intermediates and NIR detection
… to α-ketoglutarate with regeneration of reduced nicotinamide adenine dinucleotide (NADH) via malate dehydrogenase (MDH). Our specific objectives for this experiment were: (1) to examine the interaction of malate and MDH to reduce MMb in vitro, (2) to determine the influence of pH, temperature, …